1. BT-202 Netaji Subhas Institute of Technology, Dwarka, New Delhi. Dr. Amita Pandey Aug 26, 2011

5. Structure of proteins Proteins perform diverse functions in the cell.

16. peptide fragments Sequencing Edman procedure Ordering of peptides Cleaved again with different enzyme or chemical

20. Genome sequencing organism Size of genome Year Haemophilus 1.8 Mb 1995 Saccharomyces 12.1 Mb 1996 C. elegans 100Mb 1998 Fruit fly 139 Mb 2005 Human 3.5 Gb 2006

21. Consensus sequences

22. Synthesis of peptides Three ways to obtain a peptide are -from the tissue -genetic engineering -chemical synthesis

23. Chemical synthesis 9-fluorenylmethoxycarbonyl

27. Non-covalent bonds in protein folding

28. Globular protein

29. Hydrogen Bonds in Proteins

32. Protein folding  -helix – protein turns like a spiral – fibrous proteins (hair, nails, horns)  -sheet – protein folds back on itself as in a ribbon –globular protein

35. Protein structure

37. Domains

40. Single Subunit Proteins

45. Collagen and Elastin

48. Ligand Binding

52. Antibodies

58. Features of Enzyme Catalysis