1. Monitoring of Tissue Energy Metabolism and Mitochondrial Function Very New Development by Prizmatix Ltd. ISRAEL www.Prizmatix.com

2. Optical assessment of intracellular Mitochondrial Function Using NADH monitoring by the MitoViewer By Prizmatix Ltd.Israel www.Prizmatix.com Mito NADH Viewer Mitochondrion

3. Mitochondrial Function represents the energy balance of the tissue Mitochondrial Function represents the energy balance of the tissue Tissue Energy Balance Mitochondrial NADH (Fluorometry) ATP Demand Typical Examples: Kidney Function Gastrointestinal Activity Muscle Contraction Brain Ionic Homeostasis Glandular Secretion Supply Tissue Blood Flow (LDF) HbO 2 Hemoglobin Oxygenation (Oximetry) Venule Arteriole O 2 O 2 O 2 O 2 2 O 2 O 2 O 2 O 2 O 2 O 2 O 2 O 2 O 2 O 2 O 2 O 2 O 2

4. Tissue energy balance between supply and demand is reflected by the mitochondria Energy Supply Mitochondrial NADH Redox State Energy Demand 1. Ionic Homeostasis 2. Signal Conduction 3. Muscle contraction 4. Glandular Secretion 5. G-I tract and kidney Function Tissue Energy Balance TBF pO 2 HbO 2

6. Body metabolic imbalances Cancer Neuro- degenerative Diseases Nervous System Development Proliferation Therapy Parkinson’s Alzheimer’s Huntington Amyotrophic Lateral sclerosis Hepatitis C Hepatopathies Tubulopathy Transplantation Traumatic brain injury Adults Newborns & Children Spinal cord Stroke & Neuronal activity Sepsis Intensive Care Patients Neonatal Adults Liver & Kidney Human diseases related to mitochondrial activity

7. Where to Monitor NADH redox state During the last 60 years NADH was monitored almost in all organs of the body. Our group (Mayevsky et al) had published more than 150 papers in various animal models. More than 1,000 papers had been published in this area of research.

8. kidney Testis Small Intestine Liver Heart Urethra Spinal cord Animal Clinical Clinical Pigs Oxygen deficiency Ischemia NO Drugs TBI Hyperbaria HBO Clinical Activation CO Hemorrhage Hypothermia Aging Sepsis NADH Ischemia NE NE Ischemia NE Hypercapnia Papaverine Ischemia N 2 NE Hemorrhage AAA ICU Bypass Pacing Hypopnea Ischemia Drugs (Ach, NE, vasoactive) Compression Ischemia Epilepsy SD Mannitol ICP elevation Retraction Anoxia Hypoxia Hypercapnia Nimodipine Ethanol Anesthetics Uncoupler During operation ICU Brain

9. The UV light is helping us to shed new light into the darkness of Mitochondrial Functions

10. Optical assessment of intracellular Mitochondrial Function Using NADH monitoring by the MitoViewer By Prizmatix Ltd.Israel www.Prizmatix.com Mito NADH Viewer Mitochondrion

11. Detailed description of the MitoViewer components

12. 6% Oxygen Air 100% Oxygen Air Reflectance Fluorescence NADH

13. Most of the information related to NADH monitoring appears in the following review : Mayevsky, A. and Rogatsky,G.G. Mitochondrial function evaluated by NADH fluorescence: From animal models to human studies. Am J Physiol, Cell Physiol 292: C615-C640 (2007)

14. For additional information contact: Prof. Avraham Mayevsky [email_address] www.mitochondrial-medicine.com Prizmatix Ltd. POB 4234,Modiin-Ilite 71919, ISRAEL Phone: +972-8-929-7844 Fax: +972-8-929-8772 www.prizmatix.com