1. Nicole Colon Carrion
Dr. Michael Rubin
Department of Biology
University of Puerto Rico, Cayey

2.  small fly belonging to the Drosophilidae family.
 Drosophila genus contains around 1,500 species.
 They are usually found around spoiled or ripe
fruits.

3.  Many studies have used Drosophila melanogaster
as a model system for understanding human
biology and diseases.
 Ithas been estimated that nearly 75% of disease-
related genes in humans have functional orthologs
in the fly (Udai Bhan Pandey and Charles D.
Nichols, 2011; Reiter et al., 2001; Lloyd and
Taylor, 2010).

4.  native to South-East Asia
 belonging to the suzukii
subgroup.
 Adults of this fruit fly are
almost identical to Drosophila
melanogaster, with the
exception that the males have
one black spot on the back
From: FlyBase
portion of each wing. data/phylogeny/Drosophilidae-
Tree/suzukiisubgroup.htmhttp://
flybase.org/static_pages/allied-l

5.  Yellow-like genes have
only been identified in
insect species and a
number of bacteria.
 Dmely also known as the
yellow gene that encodes a
protein that functions in
melanization and behavior
in Drosophila.
Propotionated by: Science Direct
http://www.sciencedirect.com/science/a
rticle/pii/S006526600700003X

7.  Protein coding gene in charge of controlling
molecular functions.
 In D. melanogater is referred by the symbol
DmelKif3C
 It is involved in controlling microtubule motor
activity.
 Studding and annotating Kif3c can led to a better
understanding of the gene function and pathway.

8. Number of published studies
where the KIF3C gene is
over/under expressed.
Figure 1
From: EMBL-EBI
http://www.ebi.ac.uk/gxa/gene/AI107317
http://www.ebi.ac.uk/gxa/experiment/E-GEOD-27927/FBgn0039925?ef=

9.  Itis our objective to annotate the Kif3c gene
present in the Fosmid 66 of Drosophila biarmipes
using different Bioinformatics tools.
 Compare the gene annotation results of Drosophila
biarmipes with Drosophila melanogaster.

10.  Problem: whether D. melanogaster will be helpful
for the annotation of Kif3c gene of D. biarmipes.
 Hypotesis:D. melanogaster will be helpful for the
annotation of Kif3c gene of D. biarmipes

11. FlyBase Gene Record Finder
Identify possible orthologs in Isoforms and exons sequences were identified
D. biarmipes. and collected using the Gene Record Finder
BLASTX Based on the alignments chosen, the start and stop
Each exon was analyzed based on their codon of the possible gene model will
position and gene sequence using BLASTx. be identified using BLASTx.

12. Genome Browswer
Gene Model Checker
The process of joining exons was begun.
Confirm your gene model using the
Gene Model Checker.
The donor site as GT.
The acceptor site was identified
as AG.

13. Identity 233/678
(34.4%),
Similarity 266/678
(39.2%)
Gaps 371/678
(54.7%)

15. Approximate
location in project
covered by gene
(from 5’ end to 3’
end): from base
17,150 to base
23,800
Gene model contains
5 exons
Gene model
contains 23 amino
acids

16. Start and Termination Codons of the model gene from the Kif3C
gene were identify as ATG and TAA.
Start Codon Termination Codon

17.  The Kif3C gene From Fosmid 66 of Drosophila biarmipes
containing 5 exons was annotated successfully using
different Bioinformatics tools.
 The gene models were confirmed using the Gene Model
Checker.
 Start and Termination Codons of the model gene from the
Kif3C gene were identify as TGA and TAA.
 The gene annotation results of Drosophila biarmipes
were compared with Drosophila melanogaster
showing a 34.4% identity.

18.  Dr. Rubin
 Joseph Perez
 GEP