Neuromics' is a leader in providing Biopharmas, Academic and Government with CFR compliant 2 and 3-D human primary cell assays, media and supplements for discovery. We also provide antibodies, proteins/growth factors, apoptosis kits and genetic engineering/manipulation tools.

1. CHARACTERIZED, TESTED AND DISCOVERY
READY …FOR RESULTS YOU CAN TRUST AND
UNDERSTAND

2. OUR CORNERSTONE: HUMAN PRIMARY CELLS AND 3-D
TISSUE MODELS, DEFINED MEDIA AND FBS
2 and 3-D Cell
Based Assays
>250 peer
Reviewed
Publications
3-D Tissue-Like
Models
Primary Human
Astrocytes and
Primary Human
Endothelial Cells and
Pericytes
Cancer Associated
Fibroblasts and Tumor
Cells
Human MSCs and
Derived Cells
Primary Antibodies &
Markers
Apoptosis Kits
Gene Tools
Proteins and Growth
Factors
Protein Generation &
Purification
FBS, Media & Culturing
Product

3. SAMPLE CUSTOMER CELLS DATA AND RELATED PUB
Highlights Researchers using our Human Pericytes, Brain
Microvascular Endothelial Cells (HBMES) and Astrocytes to build a
static 3-D Blood-Brain Barrier Model. Ece Bayir, M. Mert
Celtikoglu, Aylin Sendemira. The use of bacterial cellulose as a
basement membrane improves the plausibility of the static in
vitro blood-brain barrier model.
https://doi.org/10.1016/j.ijbiomac.2018.12.257.
Image: Pericytes, HBMECS, and Astrocytes 5 days after culturing. Live cells
(green) and dead cells (red).
The investigators concluded, "Caffeine and sucrose
permeability values obtained from all models were close to
literature data and physiological values."

4. WIDELY USED AND FREQUENTLY PUBLISHED
Sample Publications
Catalog Number: HEC02
Ece Bayir, M. Mert Celtikoglu, Aylin Sendemir. (2018). The use of bacterial cellulose as a basement
membrane improves the plausibility of the static in vitro blood-brain barrier model. International Journal
of Biological Macromolecules. https://doi.org/10.1016/j.ijbiomac.2018.12.257
Shavali Shaik, Bridget Kennis, Shinji Maegawa, Keri Schadler, Yang Yanwen, Keri Callegari, Rishi R. Lulla,
Stewart Goldman, Javad Nazarian, Veena Rajaram, Jason Fangusaro, and Vidya Gopalakrishnan.
(2018). REST upregulates gremlin to modulate diffuse intrinsic pontine glioma vasculature. Oncotarget.
2018 Jan 12; 9(4): 5233–5250. doi: 10.18632/oncotarget.23750
Catalog Number: CAF05
Martin Nurmik, Pit Ullmann, Fabien Rodriguez, Serge Haan, Elisabeth Letellier. In search of definitions:
Cancer-associated fibroblasts and their markers. doi: 10.1002/ijc.32193
Top image: HEC02, HBMECs stained with Claudin-5
Lower image: Human CAFS-Pancreatic Stellates (CAF08) cultured in MSCGro™
supplemented with Neuromics' FBS (FBS001). Courtesy of Emily Rodela, TGEN

5. RESULTS YOU CAN TRUST AND UNDERSTAND
3rd Party Service For Customer Feedback
Neuromics review summary
Sample Reviews:
Ravi D.on BirdEye★★★★★
We have ordered Brain derived Endothelial Cells or BECs. We could not find these in different
companies, particularly from multiple donors. Overall, we are happy with the products.
Olesya P.on BirdEye★★★★★
We bought a large order of FBS from Neuromics. Our experience with their customer service was great.
They offered us the best price and worked with us on any issue issues that came up on our side.
Jiehua Z.on BirdEye★★★★★
We ordered the cells, medium and related culture product. Fast ordering and delivery procedure, and
nice technique support. The cells grew well as described and we can easily expand them.
HBMECs Stained with CD146

6. 3-D CELL-BASED ASSAY SOLUTIONS
 3D Models:
 HUVEC Model
 Left Image, HUVECS growing
an insert
 3-D Blood Brain Barrier
Models
 Retinal Angiogenesis Model
 Skin Model
 Extracellular Matrixes
• Engineered hydrogels
optimized to cell types
• Nanofibers
 Coating Solutions
 Alphabiocoat (right image)
increases cell attachment and
growth

7. HUMAN BBB MODEL TEST APPLICATIONS
• Toxicology Assays
 Permeability Studies -Compounds, Oligos and Small Molecules :
 General Procedure
 3D Blood Brain Barrier models are thawed and cultured for 4 days to establish viable barrier
as measured by transendothelial electrical resistance (> 150 Ω x cm2).
 The test compound and fluorescent probe are added and the cultures are returned to
incubate for designated times.
 If assessment of alterations to BBB permeability due to drug treatment is required,
fluorescent dextran conjugate is added to wells
 Media is sampled for quantitation of test articles by UPLC, and for quantification of
fluorecent probe by plate reader
 Apparent permeability is calculated
 Bottom Figure: Alterations in apparent permeability of blood brain barrier due to
drug treatment. Data presented as mean ± SEM x 10-6 cm/s. * Indicates p<0.05
for Fisher’s LSD t-test comparisons to DMSO controls in a post hoc analysis
following two-way ANOVA. Data Curtsey of Visokol

8. USING OUR HUMAN CELLS
 Using Neuromics brain
endothelial cells (HEC02),
pericytes (HMP104) and astrocyte
(HPM202) – Customers have
constructed their own BBB model
 TEER Assay BBB Model using
Neuromics’
 HBMVECS, Pericytes and
Astrocytes.
https://doi.org/10.1016/j.ijbiomac.
2018.12.257
 Immunofluorescence (IF) staining
results indicate that the
expression of tight and
adherence junction proteins
increases on the 5th day of the
cultivation.

9. HUMAN BRAIN ASTROCYTES
 Cat No. HMP202
 Source from non-fetal donors
 Average doubling capability of >10
 Recent publications:
 Yang Wu, Yan Li, Liangqing Zheng, Pengcheng Wang, Yan liu, Yongning
Wu, ZhiYong Gonga (2019). The neurotoxicity of Nε-
(carboxymethyl)lysine in food processing by a study based on animal
and organotypic cell culture. Ecotoxicology and Environmental Safety.
https://doi.org/10.1016/j.ecoenv.2019.110077
 Ece Bayir, M. Mert Celtikoglu, Aylin Sendemir. (2018). The use of
bacterial cellulose as a basement membrane improves the plausibility
of the static in vitro blood-brain barrier model. International Journal
of Biological Macromolecules. https://doi.org/10.1016/j.ijbiomac.2018.
12.257
 Top image: Astrocytes stained with GFAP (cat no. SP15101)
 Bottom Image: Astrocytes stained with S100 beta (cat no. GT15160)

10. HUMAN BRAIN ENDOTHELIAL CELLS
 Cat No. HEC02
 Source from non-fetal donors
 Average doubling capability of >15
 Recent publications:
 Hyun-Jung Choi, Na-Eun Kim, Il Kwon, Dukhwan Choi, Jayoung Kim, Ji Hoe
Heo. (2019). Fc-saxatilin inhibits VEGF-induced permeability by regulating
claudin-5 expression in human brain microvascular endothelial
cells. Microvascular Research. DOI:
https://doi.org/10.1016/j.mvr.2019.103953
 Larry W. Hunter, Muthuvel Jayachandran and Virginia M. Miller (2019). Sex
differences in the expression of cell adhesion molecules on microvesicles
derived from cultured human brain microvascular endothelial cells treated
with inflammatory and thrombotic stimuli. Biology of Sex
Differences 201910:26. https://doi.org/10.1186/s13293-019-0241-y
 Top image: HBMECS stained with CD146
 Bottom Image: HBMECs stained with Claudin-5

11. MORE ENDOTHELIAL CELLS
 Retinal Endothelial Cells - cat no. HEC09
 Recently publication: A. P. Da Cunha, Q. Zhang, M. Prentiss, X. Q. Wu, V.
Kainz, Y. Y. Xu, J. Vrouvlianis, H. Li, N. Rangaswamy, B. Leehy, T. L. McGee, C.
L. Bell, C. E. Bigelow, V. Kansara, Q. Medley, Q. Huang & H. Y. Wu. The
Hierarchy of Proinflammatory Cytokines in Ocular Inflammation. Current Eye
Research, Volume 43, 2018 - Issue 4 Published Online: 04 Dec 2017
 Expandable
 Top image: Retinal Endothelial Cells in Culture
 HUVECs – cat no. HEC01
 Lower Image: HUVECs cultured in collagel hydrogel
 Also available – GFP Expressing HUVECs
 Large Intestine Endothelial Cells, Coronary Artery Endothelial Cells, and
more
 Specially formulized media to support growth

12. CANCER-ASSOCIATED FIBROBLASTS
 CAFs cryopreserved at low passage with high passage capabilities
 Colorectal CAFs – cat no. CAF05
 Top Image: Colorectal CAFS fixed and permeabilized then stained with
50ng/mL Hoechst, and α -SMA (1:100). Pictures were collected by
Cytation 3 using DAPI & FITC of CAF05. Colorectal CAFs were positive
for α -SMA.
 Pancreatic CAFs – cat no. CAF08
 Melanomia CAFs – CAF09
 Breast CAFs – cat no. CAF06
 Bottom Image: Breast cancer-associated fibroblasts (Cat. No. CAF06)
were plated at 5,000/cm² in a Greiner BioOne T75 flask and maintained
in MSC-GRO low serum, complete medium (Cat. No. SC00B1) in a
reduced O2 environment (1%O2, 5%CO2, 90%N2) at 37°C in a
humidified chamber.

13. GFP OR RFP EXPRESSING CARCINOMA CELL
LINES
 Gastric Carcinoma 87
 Top image: RFP Expressing Gastric Carcinoma 87 Cells – cat no. TR02-
RFP
 Glioblastoma
 Prostate Carcinoma Cells (DU 145)
 Renal Adenocarcinoma Cells (ACHN)
 Bottom image: GFP-Expressing Renal Adenocarcinoma Cells – cat no.
TR04-GFP

14. MESENCHYMAL STEM CELLS
 Cord Blood Derived – cat no. MSC001
 5-10 passages
 Top Graph: MSCs expanded through 15 serial passages. Cells were
plated at 5,000/cm² in a T25 flask and maintained in MSC-GRO low
serum, complete medium (cat# SC00B1) in a reduced O₂ environment
(1%O₂, 5%CO₂, 90%N₂) at 37°C in a humidified chamber. Cells were
detached in Accutase when cultures reached ~ 80 TO 90% confluence.
 Bone Marrow Derived – cat no. SC00BM1 and MSC001
 Placental Derived – cat no. SC00P1
 Passaged 5 to 10 passages in our Low Serum, Complete Medium
(#SC00B1)
 Adipose Derived – cat no. SC00AD1
 Human Adipose derived Mesenchymal Stem cells in culture. Cells were
plated at 5,000/cm² in a T75 flask and maintained in cat# SC00B1
media in a reduced O₂ environment at 37°C in a humidified chamber.

15. Phone: 952-374-616
Fax: 612-677-3976
Email: brett@neuromics.com for orders
rose@neuromics.com for tech questions
We welcome your input, feedback and questions:
Pete Shuster-CEO and Owner
Direct Phone: 612-801-1007
Email: pshuster@neuromics.com
5235 W 74th Street Suite 8
Edina, MN, 55439
www.neuromics.com
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