محاضرة الدكتورة نيفين الخشاب المقامة في مركز البحوث ضمن سلسلة أفق بحثية مميزة

1. 9/16/15
1
Electrochemical
pH
Ionic Strength
Biological
Mechanical Electrical
Magnetic
Light
Temperature
What is “SMART”?
External
S+muli
Internal
S+muli

2. 9/16/15
2
Increase stability and
promote an ordered
architecture
Decrease
extensive surface
functionalization
for eventual
degradation
Hard
Soft
Inorganic
Organic
Hybrid Materials
Hybrid Materials

3. Yang, X.; Moosa, B.; Deng, L.; Zhoa, L; Khashab, N. M. *
“Polymer Chemistry 2011, 2, 2543–2547.
Biomedical: pH-Triggered Micellar Membrane for
Controlled Release Microchips
!
0 5 10 15 20
0
10
20
30
40
50
b
time (h)
DOXrelease(wt%)
pH=4.0
pH=5.0
pH=6.0
pH=7.0
pH=8.0
pH=9.0
!
General Scheme
Assembly and Application

4. Deng, Lin; Wang, Guangchao; Ren, Jian; Zhang, Bei; Yan, Jingjing; Li, Wengang; Khashab, N. M. * RSC adv. 2012, 2,
12909–12914
Biomedical: Enzymatically Triggered System
Based on Hyaluronic Acid Micelles
General Scheme

5. Deng, Lin; Wang, Guangchao; Ren, Jian; Zhang, Bei; Yan, Jingjing; Li, Wengang; Khashab, N. M. * RSC adv. 2012, 2,
12909–12914.
Biomedical: Enzymatically Triggered System
Based on Hyaluronic Acid Micelles

6. Biomedical: Magnetically Triggered Theranostic Platform
for Remote Cancer Cells Therapy and Imaging
!
General Scheme
Zhang, W.; Deng, L.; Wang, G.; Guo, X.; Li, Q.; Zhang, J.; Khashab, N. M. * Part. Pat. Syst. Charact. 2014, 31, 985–993.

7. Biomedical: Magnetically Triggered Theranostic Platform
for Remote Cancer Cells Therapy and Imaging
!
Zhang, W.; Deng, L.; Wang, G.; Guo, X.; Li, Q.; Zhang, J.; Khashab, N. M. * Part. Pat. Syst. Charact. 2014, 31, 985–993.

8. Biomedical: Photo-Triggered Membrane Based on Inter-
particle Crosslinking for Decreasing Diffusion Rates
PHCA microspheres
EC membrane
IrradiationDrug Reservoir Drug Reservoir
Lower Efflux
Crosslinking
(A)
Membrane
PMMA device
Reservoir for
drug loading
(B)
(C1) (C3)(C2)
b
c
a
d
(A)
(B)
Song, L., Moosa, M., Li, W.; Khashab, N. M.* J. Mater. Chem. B 2015, 3, 1208–1216.

9. Deng, L.; Li, Q.; Yang, Y.;Omar, H; Tang, N.; Zhang,J.; Nie, Z.; Khashab, N. M.* Chem. Eur. J. 2015, accepted (chem.201502522)
.
Biomedical: Graphene Oxide-Wrapped Gold
Nanorods
General Scheme
!
“Two-Step” Raman Imaging-Guided Chemo-Photothermal Cancer Therapy

10. Deng, L.; Li, Q.; Yang, Y.;Omar, H; Tang, N.; Zhang,J.; Nie, Z.; Khashab, N. M.* Chem. Eur. J. 2015, accepted (chem.201502522)
Biomedical: Graphene Oxide-Wrapped Gold
Nanorods
!

11. Deng, L.; Li, Q.; Yang, Y.;Omar, H; Tang, N.; Zhang,J.; Nie, Z.; Khashab, N. M.* Chem. Eur. J. 2015, accepted (chem.201502522)
.
Biomedical: Graphene Oxide-Wrapped Gold
Nanorods
SERS mapping of Doxorubicin (a) and
Graphene oxide (b) on AuNRs, ratio of
Doxorubicin to graphene oxide (c), and
overlap image (d) in Hela cell

12. Deng, L.; Li, Q.; Yang, Y.;Omar, H; Tang, N.; Zhang,J.; Nie, Z.; Khashab, N. M.* Chem. Eur. J. 2015, accepted (chem.201502522)
.
Biomedical: Graphene Oxide-Wrapped Gold
Nanorods

13. Biomedical: Photoresponsive Bridged Silsesquioxane
Nanoparticles with Tunable Morphology
1hν
laserH
+
+
+
+
+ +
+
+
++
+
-
-
-
- -
-
-
- -
- -
-
-
-
--
- -
- -
-
-
- -
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
- -
-
-
-
-
- - -
-
-
Fatieiev, Y.; Croissant, J.; Julfakyan, K.; Deng, L.; Anjum, D.H.; Gurinov, A.; Khashab, N.M. Nanoscale 2015, DOI: 10.1039/
C5NR03065J

14. BS NPs
Hollow BS NPs
200 nm
100 nm
H
+
+
+
+
+ +
+
+
++
+
H
+
+
+
+
+ +
+
+
++
+
H2O
H2O
EtOH

15. Origin
Irradiated
Or
e
Irra
e
4.05 ppm
Irra
e
4.00
3.75
Irradiated
Or
e
4.00
3.75
O
+ − +
O
−
+
1hν
laser
1hν
laser
Positive Zeta potential Negative Zeta potential
Fatieiev, Y.; Croissant, J.; Julfakyan, K.; Deng, L.; Anjum, D.H.; Gurinov, A.; Khashab, N.M. Nanoscale 2015, DOI: 10.1039/
C5NR03065J

16. Hollow BS NPs
DNA
1hν
laser
Zeta Potential (mV)
ChargeDist.(a.u.)
-100 -50 0 50 100
-39 mV
+46 mV
1hν
H
+
+
+
+
+ +
+
+
++
+
-
-
-
-
-
-
-
-
-
-
-
-
- -
--
-
-
-
- -
- - -
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
- -
-
-
-
-
- - -
-
-
H
+
+
+
+
+ +
+
+
++
+
Fatieiev, Y.; Croissant, J.; Julfakyan, K.; Deng, L.; Anjum, D.H.; Gurinov, A.; Khashab, N.M. Nanoscale 2015, DOI: 10.1039/
C5NR03065J

17. Angew. Chem. Int. Ed. 2015, accepted DOI: 10.1002/anie.201501615R1 and 10.1002/ange.201501615R1 (Cover image, Highlighted
by Nature Middle East)
Biomedical: Light Triggered Colloidosomes
-
+
-
+
-+
-
+-+
-
+
-
+
-
+
- +
-
+ - +
-
+
-
-
-
-
--
-
---
-
-
-
-
-
-
- -
-
- - -
-
-
hv
Stable colloidosome
Unstable colloidosomeDissociation of colloidosome
NBSN-2
NBSN-1
-
+
+
- -+
-+
+
-
+ -
-
+
+
-
+
Oil
phase Water
phase
Emulsion with two types of NPs
A
-
--
-
--
-
-
-
-
-
-
-
- -
--
-
- -
-
-
-
-
C
B

18. -100
-80
-60
-40
-20
0
20
40
60
80
100
Before Irradiation After Irradiation
SN
(control)
Zeta
Potential
(mV)
NBSN-1 NBSN-2 S
(c
hν
Positive
Neutral
- -
+
NP- NP+NB+
SURFACE CHARGE OF NP- & NP+ NANOBUILDING BLOCKS
COMPATIBLE WITH THE COLLOIDOSOME ASSEMBLY/DISASSEMBLY
28
Biomedical: Light Triggered Colloidosomes

19. 3
µm
500
nm
500
nm
200
nm
29

20. hν
500
nm
ELECTROSTATIC DISASSEMBLY OF COLLOISOMES
UPON LASER IRRADIATION
30

21. 0
20
40
60
80
100
0
5
10
15
20
Release
(%)
Time
(min)
Cargo loaded
Colloidosomes
OFF ON OFF ON
hν
hν
hν
31
Angew. Chem. Int. Ed. 2015, accepted DOI: 10.1002/anie.201501615R1 and 10.1002/ange.201501615R1 (Cover image, Highlighted
by Nature Middle East)

22. Biomedical: AuNC@BSA Gated MSNs
+
+
+
+
+
+
+
+
Fluorescent
AuNC@BSAn-
Cargo3loaded
MSN3NH3
+
+
-
+
+
+
+
+
+
+
--
+
+
+
+
+
+
+
++
+
H+
pH 7.4
pH 5.0
Release9(%)
pH 5.0
pH 7.4
b
Time9(h)
a
!

23. a b
3 µm
c d
50 nm 50 nm
100 nm
!
Size%Distribution%(%)
Diameter%(nm)
2.5%nm
!
Biomedical: AuNC@BSA Gated MSNs

24. Cell
Viability
(%)
NPs
Concentration
(µg.mL-­‐1)
100
80
60
40
20
0
0
10
20
30
50
100
150
Cell$Viability$(%)
MSN$NH2+DOX
MSN$AuNC@BSA+DOX
DOX$Concentration$(µg.mL91) !
Biomedical: AuNC@BSA Gated MSNs

25. !
Absorption
Wavelength1(nm)
Emission
645$nm364$nm
496$nm
!
Biomedical: AuNC@BSA Gated MSNs

26. Red$Channel$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$Blue$Channel$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$Merged
Saline
Control
Hoe4loaded$
MSN4AuNC@BSA
a
b
!
Biomedical: AuNC@BSA Gated MSNs

27. S
L
T
K
Lu
Saline
Control
a Merged222222222222222222222222Red2Channel22222222222 Blue2Channel2 Red+Blue2Channel22222222
L
T
K
Lu
b
Hoe9loaded2
MSN9AuNC@BSA
!
Submitted
Biomedical: AuNC@BSA Gated MSNs

28. Sensing: Protein-Directed Synthesis of Highly
Fluorescent Gold Nanoclusters
Xie, J.; Zheng, Y.; Ying, J. Y. J. Am. Chem. Soc., 2009, 131, 888

29. Sensing: Protein-Directed Synthesis of Highly
Fluorescent Gold Nanoclusters
Hu, L.; Deng, L.; Alsaiari, S.; Zhang, D.; Khashab, N. M. Anal. Chem., 2014, 86, 4989–4994.

30. Sensitivity
Selectivity
Sensing: Protein-Directed Synthesis of Highly
Fluorescent Gold Nanoclusters
Hu, L.; Deng, L.; Alsaiari, S.; Zhang, D.; Khashab, N. M. Anal. Chem., 2014, 86, 4989–4994.

31. Sensing: Protein-Directed Synthesis of Highly
Fluorescent Gold Nanoclusters
Hu, L.; Deng, L.; Alsaiari, S.; Zhang, D.; Khashab, N. M. Anal. Chem., 2014, 86, 4989–4994.

32. Sensing: Protein-Directed Synthesis of Highly
Fluorescent Gold Nanoclusters
Hu, L.; Deng, L.; Alsaiari, S.; Zhang, D.; Khashab, N. M. Anal. Chem., 2014, 86, 4989–4994.

33. Sensing: Protein-Directed Synthesis of Highly
Fluorescent Gold Nanoclusters
Hu, L.; Deng, L.; Alsaiari, S.; Zhang, D.; Khashab, N. M. Anal. Chem., 2014, 86, 4989–4994.

34. Sensing: Protein-Directed Synthesis of Highly
Fluorescent Gold Nanoclusters
Hu, L.; Deng, L.; Alsaiari, S.; Zhang, D.; Khashab, N. M. Anal. Chem., 2014, 86, 4989–4994.

35. Biomedical: Zippered Release from Polymer
Gated Carbon Nanotubes
!
Mashat, A.; Deng, L; Altawashi, A.; Sougrat, R.; Wang, G.; Khashab, N. M. * “Zippered Release from Polymer Gated
Carbon Nanotubes” J. Mater. Chem. 2012, 22, 11503–11508.
General Scheme

36. Biomedical: Zippered Release from Polymer
Gated Carbon Nanotubes
Mashat, A.; Deng, L; Altawashi, A.; Sougrat, R.; Wang, G.; Khashab, N. M. * “Zippered Release from Polymer Gated
Carbon Nanotubes” J. Mater. Chem. 2012, 22, 11503–11508.
!

37. Chen, Ye; Tao, Jing; Deng, Lin; Li, Liang; Li, Jun; Yang, Yang; Khashab, N. M.* ACS App. Mater. Inter. 2013, 5, 7478–
7484. .
Composites: PEI/Bucky Gels Nanocomposites
with Superior Conductivity and Thermal Stability
!
General Scheme

38. Chen, Ye; Tao, Jing; Deng, Lin; Li, Liang; Li, Jun; Yang, Yang; Khashab, N. M.* ACS App. Mater. Inter. 2013, 5, 7478–7484.
Composites: PEI/Bucky Gels Nanocomposites
with Superior Conductivity and Thermal Stability
!

39. Chen, Ye; Tao, Jing; Song, Li; Khashab, N. M. * ACS App. Mater. Inter. 2014, 6, 9013–9022.
Composites: Polyfluorene Wrapped Carbon
nanotubes for Enhanced Interfacial Interactions
General Scheme

40. Chen, Ye; Tao, Jing; Song, Li; Khashab, N. M. * ACS App. Mater. Inter. 2014, 6, 9013–9022.
Composites: Polyfluorene Wrapped Carbon
nanotubes for Enhanced Interfacial Interactions

41. Composites: Surface Modification of Multiwall Carbon
Nanotubes with Cationic Conjugated Polyelectrolytes
General Scheme
Ezzeddine, A.; Chen, Z.; Schanze, K. S.; Khashab, N. M. ACS Appl. Mater. Interfaces, 2015, 7 , 12903–12913.

42. Composites: Surface Modification of Multiwall Carbon
Nanotubes with Cationic Conjugated Polyelectrolytes
Ezzeddine, A.; Chen, Z.; Schanze, K. S.; Khashab, N. M. ACS Appl. Mater. Interfaces, 2015, 7 , 12903–12913.

43. KAUST
on
the
Red
Sea
Spring 2016 internships and graduate students
Applications
are now open for submission
http://visitingstudent.kaust.edu.sa/Pages/Home.aspx
Niveen.khashab@kaust.edu.sa