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SEMIAR ON
WWW. ITS USAGE IN THE BIOTECHNOLOGY
By
Ashok Sidar
M.Sc. 2nd semester
SOS IN BIOTECHNOLOGY
SYNOPSIS
WWW=WORLD WIDE WEB.
It is a collection of all the websites connected via
internet worldwide & it has become known simply as
the WEB.
The WWW was central to the development of the
INFORMATION AGE.
It is the base of server.
(Server is a computer program or a machine that waits for request
from other machines or software & responds to them. It typically
processes data.)
(Software-It is a set of instructions that directs a computer to perform
specific operations.)
INTRODUCTION
Sir Tim Berners Lees is father of World Wide
Web.
He discovered WWW in the year 1989.
By October 1990, he had written the 3
fundamental technologies that remain the
foundation of the today’s Web:
1) HTML
2) URI/URL
3) HTTP
1) HTML (Hyper Text Markup Language)
The markup (formatting) language for the web
2)URI/URL (Uniform Resource Identifier/Locator)
A kind of address that is unique & used to identify
to each resource on the web. It is commonly
called a URL
3) HTTP (Hypertext Transfer Protocol) – It is the
protocol to exchange or transfer hypertext
A network is a group of two or more
computer systems linked together.
- A computer network can
be defined as interconnection of two or more
computers with the objective of sharing data,
resources and communication.
LAN : [ The computers are
geographically close together (i.e., in the same
building).
WAN : [ The computes are
farther apart & are connected by telephone lines
or radio waves ].
MAN : [ A data
network designed for a town or city ].
CAN : [ The computers are
within a limited geographic area, such as a
campus or military base ].
INTERNET APPLICATIONS
INTERNET-It refers to collection of computers
in the network to exchange information and
data using common software.
 WWW
 E-MAIL
 VIDEO CONFERENCING
 SOCIAL NETWORKING
 CHATTING
 ONLINE EXAMINATION
 It is a subset of Internet & presents text,
images, animations, video, sound & other
multimedia in a single interface.
 We are able to see the research papers and journals of
any subject.
 There are many scientific sites to get information.
 Internet is very essential in Bioinformatics for
sequencing amino acids and nucleotides.
 Internet makes research easy, time saving and more
affordable making people more interested.
• A Search Engine is a program that searches through a
database of web pages for particular information.
• Some popular search engines :
Search Engines/Directory URL
Bing.com http://www.bing.com
Ask.com http://www.ask.com
About.com http://www.about.com
Khoj.com http://www.khoj.in
Yahoo.com http://www.yahoo.com
Q. What is a website?
A. A website is a collection of world wide web
files, scripts, images, multimedia files, audio-
video files.
Site refers to ‘place’ and ‘web’ refers to
collection of files online.
1) NUCLEOTIDE SEQUENCE COMPARISON:-
Nucleotide sequence(DNA/RNA) in suitable format
Select BLAST programme
Select database to be used for comparison
Sequence submitted to BLAST server
Homology sequence
Output file
A database is a vast collection of data
pertaining to a specific topic, e.g.,
nucleotide sequence, protein sequence,
etc. in an electronic environments.
DATABASE INFORMATION
AVAILABLE
SOURCE
GenBank Nucleotide
sequences
NCBI,USA
Mito Mitochondrial DNA
sequences
NCBI,USA
EMBL Nucleotide
Sequence Databank
(European
Molecular Biology
Laboratory)
DNA
sequences
EBI,USA
Yeast Yeast genomic
sequences
NCBI,USA
 NCBI – National Centre for Biotechnology
Information. It is held at USA.
EBI – European Bioinformatics Institute held at
U.K.
BLAST (Basic Local Alignment Search Tool)
It is a family of user-friendly sequence
similarity search tools on the web.
The BLAST server is supported through
NCBI,USA.
There are several types of
BLAST programmes
 BLASTp – It compares submitted protein
sequence against a protein database.
 BLASTx – This programme translates the
submitted nucleotide sequence into amino
acid sequence and compares the latter with a
protein database.
 tBLASTn – The programme converts the
submitted protein sequence & compares it
with a nucleotide sequence database.
 BLASTn – This is used to compare a nucleotide
sequence with a nucleotide sequence
database.
 tBLASTx – This programme translates the
submitted nucleotide sequence as well as the
nucleotide sequence database into amino acid
sequences and searches for homology
between the two.
2) SOME INDIAN DATABASES :
• GM Crops Database
• Vanshanudhan
3) MOLECULAR PHYLOGENETICS
The DNA & protein sequence data can be used
to investigate evolution of genes and their
protein products; this is called molecular
phylogeny.
Search tools (like BLAST) are used to
determine the phylogenetic relationshiphs,
which may be presented either in form of a
phylogenetic tree or a dendogram.
4) IDENTIFICATION OF FUNCTION OF A
NEW GENE
The gene sequence is translated into the
amino acid sequence of the protein. This
protein sequence is then compared with a
protein database. A programme like tBLASTx
will perform both these functions. If the
encoded protein is homologous to a protein in
the database, it confirms the identification of
the new gene.
5) ONLINE JOB SEARCHING
 www.irri.org/ : Work for rice research.
 Jobsearch.naukri.com/biotech-jobs-in-indore
 www.indeed.co.in/Biotechnology-job-in-
Madhya Pradesh.
 in.linkedin.com > Jobs > Biotechnology Jobs
 www.biotechnologyjobs.com
 www.timesjobs.com > Biotechnology Jobs
6) ONLINE SHOPPING OF
BIOTECHNOLOGY INSTRUMENTS
 www.microbiopharm.com/
 www.reagecon.com/life-science-
biotechnology.html.
 https://www.novabiotech.com
 www.carolina.com > Biotechnology
7) JOURNALS AND RESEARCH PAPERS
1) researchinbiotechnology.com/
It is an open access online journal that provides
rapid publication of articles in all areas of
Biotechnology.
2) http://www.springeropen.com/journals/bysubject
This website helps us to search journals according
to your subjects.
3) http://www.ncbi.nlm.nih.gov/pubmed
PubMed comprises more than 25millions
citations for bio-medial literatures from
MEDICINE, life sciences journals, & online
books. Citations may include links to fulltext
content from PubMed Central & publisher
websites.
4)http://medial.nic.in/
It provides free full text articles published in
select Indian Bio-medial Journals indexed in
IndMed or PubMed.
 WWW is the search engine of computer
using for different Information.
 Internet is very useful in higher education,
research and Information.
MICROSOFT – Saksham (www.sakshat.com)
BIOTECHNOLOGY EXPANDING HORIZONS(4TH
Edition)
-B.D.Singh
www.webopedia.com (Date:24/1/2016)
http://study.com/academy/lesson/types of
networks-lan-wan-wlan-man-san-pan-epn-
vpn.html (Date:24/1/2016)
THANK YOU

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WWW in biotechnology

  • 1. SEMIAR ON WWW. ITS USAGE IN THE BIOTECHNOLOGY By Ashok Sidar M.Sc. 2nd semester SOS IN BIOTECHNOLOGY
  • 3. WWW=WORLD WIDE WEB. It is a collection of all the websites connected via internet worldwide & it has become known simply as the WEB. The WWW was central to the development of the INFORMATION AGE. It is the base of server. (Server is a computer program or a machine that waits for request from other machines or software & responds to them. It typically processes data.) (Software-It is a set of instructions that directs a computer to perform specific operations.) INTRODUCTION
  • 4. Sir Tim Berners Lees is father of World Wide Web. He discovered WWW in the year 1989. By October 1990, he had written the 3 fundamental technologies that remain the foundation of the today’s Web: 1) HTML 2) URI/URL 3) HTTP
  • 5. 1) HTML (Hyper Text Markup Language) The markup (formatting) language for the web 2)URI/URL (Uniform Resource Identifier/Locator) A kind of address that is unique & used to identify to each resource on the web. It is commonly called a URL 3) HTTP (Hypertext Transfer Protocol) – It is the protocol to exchange or transfer hypertext
  • 6. A network is a group of two or more computer systems linked together.
  • 7. - A computer network can be defined as interconnection of two or more computers with the objective of sharing data, resources and communication.
  • 8. LAN : [ The computers are geographically close together (i.e., in the same building). WAN : [ The computes are farther apart & are connected by telephone lines or radio waves ]. MAN : [ A data network designed for a town or city ]. CAN : [ The computers are within a limited geographic area, such as a campus or military base ].
  • 9. INTERNET APPLICATIONS INTERNET-It refers to collection of computers in the network to exchange information and data using common software.  WWW  E-MAIL  VIDEO CONFERENCING  SOCIAL NETWORKING  CHATTING  ONLINE EXAMINATION
  • 10.  It is a subset of Internet & presents text, images, animations, video, sound & other multimedia in a single interface.
  • 11.
  • 12.  We are able to see the research papers and journals of any subject.  There are many scientific sites to get information.  Internet is very essential in Bioinformatics for sequencing amino acids and nucleotides.  Internet makes research easy, time saving and more affordable making people more interested.
  • 13. • A Search Engine is a program that searches through a database of web pages for particular information. • Some popular search engines : Search Engines/Directory URL Bing.com http://www.bing.com Ask.com http://www.ask.com About.com http://www.about.com Khoj.com http://www.khoj.in Yahoo.com http://www.yahoo.com
  • 14. Q. What is a website? A. A website is a collection of world wide web files, scripts, images, multimedia files, audio- video files. Site refers to ‘place’ and ‘web’ refers to collection of files online.
  • 15. 1) NUCLEOTIDE SEQUENCE COMPARISON:- Nucleotide sequence(DNA/RNA) in suitable format Select BLAST programme Select database to be used for comparison Sequence submitted to BLAST server Homology sequence Output file
  • 16. A database is a vast collection of data pertaining to a specific topic, e.g., nucleotide sequence, protein sequence, etc. in an electronic environments.
  • 17. DATABASE INFORMATION AVAILABLE SOURCE GenBank Nucleotide sequences NCBI,USA Mito Mitochondrial DNA sequences NCBI,USA EMBL Nucleotide Sequence Databank (European Molecular Biology Laboratory) DNA sequences EBI,USA Yeast Yeast genomic sequences NCBI,USA
  • 18.  NCBI – National Centre for Biotechnology Information. It is held at USA. EBI – European Bioinformatics Institute held at U.K.
  • 19. BLAST (Basic Local Alignment Search Tool) It is a family of user-friendly sequence similarity search tools on the web. The BLAST server is supported through NCBI,USA.
  • 20. There are several types of BLAST programmes  BLASTp – It compares submitted protein sequence against a protein database.  BLASTx – This programme translates the submitted nucleotide sequence into amino acid sequence and compares the latter with a protein database.  tBLASTn – The programme converts the submitted protein sequence & compares it with a nucleotide sequence database.
  • 21.  BLASTn – This is used to compare a nucleotide sequence with a nucleotide sequence database.  tBLASTx – This programme translates the submitted nucleotide sequence as well as the nucleotide sequence database into amino acid sequences and searches for homology between the two.
  • 22. 2) SOME INDIAN DATABASES : • GM Crops Database • Vanshanudhan
  • 23. 3) MOLECULAR PHYLOGENETICS The DNA & protein sequence data can be used to investigate evolution of genes and their protein products; this is called molecular phylogeny. Search tools (like BLAST) are used to determine the phylogenetic relationshiphs, which may be presented either in form of a phylogenetic tree or a dendogram.
  • 24.
  • 25. 4) IDENTIFICATION OF FUNCTION OF A NEW GENE The gene sequence is translated into the amino acid sequence of the protein. This protein sequence is then compared with a protein database. A programme like tBLASTx will perform both these functions. If the encoded protein is homologous to a protein in the database, it confirms the identification of the new gene.
  • 26. 5) ONLINE JOB SEARCHING  www.irri.org/ : Work for rice research.  Jobsearch.naukri.com/biotech-jobs-in-indore  www.indeed.co.in/Biotechnology-job-in- Madhya Pradesh.  in.linkedin.com > Jobs > Biotechnology Jobs  www.biotechnologyjobs.com  www.timesjobs.com > Biotechnology Jobs
  • 27. 6) ONLINE SHOPPING OF BIOTECHNOLOGY INSTRUMENTS  www.microbiopharm.com/  www.reagecon.com/life-science- biotechnology.html.  https://www.novabiotech.com  www.carolina.com > Biotechnology
  • 28. 7) JOURNALS AND RESEARCH PAPERS 1) researchinbiotechnology.com/ It is an open access online journal that provides rapid publication of articles in all areas of Biotechnology. 2) http://www.springeropen.com/journals/bysubject This website helps us to search journals according to your subjects.
  • 29. 3) http://www.ncbi.nlm.nih.gov/pubmed PubMed comprises more than 25millions citations for bio-medial literatures from MEDICINE, life sciences journals, & online books. Citations may include links to fulltext content from PubMed Central & publisher websites. 4)http://medial.nic.in/ It provides free full text articles published in select Indian Bio-medial Journals indexed in IndMed or PubMed.
  • 30.  WWW is the search engine of computer using for different Information.  Internet is very useful in higher education, research and Information.
  • 31. MICROSOFT – Saksham (www.sakshat.com) BIOTECHNOLOGY EXPANDING HORIZONS(4TH Edition) -B.D.Singh www.webopedia.com (Date:24/1/2016) http://study.com/academy/lesson/types of networks-lan-wan-wlan-man-san-pan-epn- vpn.html (Date:24/1/2016)