SANJAY LC-MSMS SPEC.pptx

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sanjayudps2016

Mass spectrometry is a technique used to identify the mass of molecules and their fragments. It works by breaking molecules into smaller fragments and measuring their mass-to-charge ratios. The document discusses the principles of mass spectrometry and fragmentation patterns used for identification.

Salud y medicina

MASS
SPECTROSCOPY
BY- ASST. PROF. S. R. UCHIBAGLE

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3.1 Introduction 3.2 Principle of infra-red spectroscopy 3.3 Theory—Molecular Vibrations 3.4 Vibrational Frequency 3.5 Number of Fundamental Vibrations 3.6 Selection Rules (Active and Forbidden Vibrations) 3.7 Factors Influencing Vibrational Frequencies 3.8 Scanning of Infra-red Spectrum (Instrumentation) 3.9 Sampling Techniques 3.10 Finger Print Region 3.11 Spectral Features of Some classes of organic Compounds 3.11 A1 Alkanes and Alkyl residues 3.11 A2 Alkenes 3.11 A3 Alkynes 3.11 A4 Cycloalkanes 3.11 A5 Aromatic Hydrocarbons 3.11 B Halogen Compounds 3.11 C Alcohols and Phenols 3.11 D Ethers 3.11 E Carbonyl compounds 3.11 E1 Aldehydes and Ketones 3.11 F Esters and Lactones 3.11 G Carboxylic Acids 3.11 H Acid Halides 3.11 I Acid Anhydrides 3.11 J Amides 3.11 K Lactams 3.11 L Amino Acids

INFRA RED SPECTROSCOPY.pptx

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UV-VISIBLE SPECTROSCOPY. 1. Introduction 2. Absorption Law (Beers & Lamberts Law) 3. Theory of Electronic Spectroscopy 1. Types of Electronic Transitions (K, R, B, E Bands) 2. The Chromophore Concept 3. Auxochrome 4. Absorption and Intensity Shifts a. Bathochromic effect. (Red Shift) b. Hypsochromic shift (Blue Shift) c. Hyperchromic effect (Abs Increase) d. Hypochromic effect (Abs Decrease) 5. Effect of Temperature and Solvent on the Fineness of Absorption Band. 6. Woodward-fieser Rules for Calculating Absorption Maximum in Dienes 7. Instrumentation. A. Light Source b. Collimating System c. Monochromator d. Sample Holder e. Detector.

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