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C A M E R A A Metagenomics Resource  for Microbial Ecology Saul A. Kravitz J. Craig Venter Institute Rockville, Maryland USA KNAW Colloquium May 29, 2008
Goals ,[object Object],[object Object],[object Object]
Presentation Outline ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Metagenomic Questions
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Genomics vs Metagenomics
Complexity of  Microbial Communities ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Global Ocean Sampling Expedition
Global Ocean Sampling (GOS) ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],3/08 3/07 4/04
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],GOS:  Sequence Diversity in the Ocean Rusch et al  (PLoS 2007)
Comparing of Dominant Ribotypes
Comparison of  Total Genomic Content
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],GOS Protein Analysis   Yooseph et al (PLoS 2007)
Rubisco homologs Added Protein Family Diversity Yooseph et al (PLoS 2007)   New Groups GOS prokaryotes Known eukaryotes Known prokaryotes
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],GOS Viral Analysis (Williamson et al PLoSOne 2008)
Viral acquisition of host genes talC Gene   GOS Viral Public Viral GOS Bacterial Public Bacterial Public Euk
Reference Genomes ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Motivations for CAMERA
Convention on Biological Diversity ,[object Object],[object Object],[object Object],[object Object],[object Object]
CAMERA – http://camera.calit2.net ,[object Object],[object Object],[object Object],[object Object],[object Object],[email_address]
CAMERA Capabilities ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
CAMRA Compute and Storage Complex at UCSD/Calit2 512 Processors  ~5 Teraflops  ~ 200 Terabytes Storage  Source:  Larry Smarr, Calit2
CAMERA Metagenomic Data Volume  by Project
CAMERA Metagenomic Samples
CAMERA Users >2000 Registered Since March 2007
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],CAMERA Data Collections
Standardizing Contextual Metadata ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Contextual Metadata Challenges ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
CAMERA Core Metadata by Project ,[object Object],[object Object],[object Object],[object Object],[object Object]
CAMERA Contextual Metadata
CAMERA 1.3 http://camera.calit2.net
Scalable BLAST with Metadata ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Scalable BLAST with Metadata
Integration of Metadata and Data
Browsing Large Data Collections: Fragment Recruitment Viewer ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Fragment Recruitment Viewer Sequence Similarity Genomic Position Doug Rusch, JCVI
Sequence Similarity Genomic Position Annotation Geographic Legend
 
 
 
Prochlorococcus marinus str. MIT 9312 ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Mate Status Highlights Differences ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Mate Pairs Highlight Variation
What Genes are Involved
View by Sample
View by Sample Filter by mate status
Annotation of Environmental Shotgun Data ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Protein Clusters as Gene Finder ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Protein Clusters Advantages and Disadvantages ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Search for Dehalogenase
Browse Clusters
Near Future ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Credits ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],http://camera.calit2.net

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CAMERA Presentation at KNAW ICoMM Colloquium May 2008

Notas del editor

  1. Why CAMERA? CAMERA Capabilities CAMERA Challenges CAMERA Directions