1. Control of
Prokaryotic (Bacterial) Genes
AP Biology 2007-2008
2. Bacterial metabolism
Bacteria need to respond quickly to
changes in their environment
if they have enough of a product,
need to stop production
why? waste of energy to produce more
STOP
how? stop production of enzymes for synthesis
if they find new food/energy source,
need to utilize it quickly
why? metabolism, growth, reproduction
GO
how? start production of enzymes for digestion
AP Biology
3. Remember Regulating Metabolism?
Feedback inhibition
- = inhibition
product acts
as an allosteric
inhibitor of
1st enzyme in tryptophan
pathway
but this is wasteful
production of enzymes -
Oh, I
remember this
from our
Metabolism Unit!
AP Biology
4. Different way to Regulate Metabolism
Gene regulation
instead of blocking
- = inhibition
enzyme function,
block transcription
of genes for all
enzymes in
tryptophan pathway
saves energy by
not wasting it on
unnecessary
-
protein synthesis -
Now, that’s a
good idea from a
AP Biology lowly bacterium!
5. Gene regulation in bacteria
Cells vary amount of specific enzymes
by regulating gene transcription
turn genes on or turn genes off
turn genes OFF example
if bacterium has enough tryptophan then it
STOP doesn’t need to make enzymes used to build
tryptophan
turn genes ON example
if bacterium encounters new sugar (energy
GO source), like lactose, then it needs to start
making enzymes used to digest lactose
AP Biology
6. Bacteria group genes together
Operon
genes grouped together with related functions
example: all enzymes in a metabolic pathway
promoter = RNA polymerase binding site
single promoter controls transcription of all genes in
operon
transcribed as one unit & a single mRNA is made
operator = DNA binding site of repressor protein
AP Biology
7. So how can these genes be turned off?
Repressor protein
binds to DNA at operator site
blocking RNA polymerase
blocks transcription
AP Biology
8. Operon model
Operon:
operator, promoter & genes they control
RNA
polymerase serve as a model for gene regulation
RNA
TATA repressor
polymerase gene1 gene2 gene3 gene4 DNA
mRNA 1 2 3 4
enzyme1 enzyme2 enzyme3 enzyme4
promoter operator
Repressor protein turns off gene by repressor = repressor protein
blocking RNA polymerase binding site.
AP Biology
9. Repressible operon: tryptophan
Synthesis pathway model
RNA When excess tryptophan is present,
polymerase it binds to tryp repressor protein &
triggers repressor to bind to DNA
blocks (represses) transcription
RNA
TATArepressor
polymerase
trp gene1 gene2 gene3 gene4 DNA
mRNA 1 2 3 4 trp trp
trp trp
enzyme1 enzyme2 enzyme3 enzyme4
promoter operator trp trp
repressor repressor protein
trp
trp
trp tryptophan trp
conformational change in tryptophan – repressor protein
AP Biology
repressor protein! trp repressor
complex
10. Tryptophan operon
What happens when tryptophan is present?
Don’t need to make tryptophan-building
enzymes
Tryptophan
AP Biology is allosteric regulator of repressor protein
11. Inducible operon: lactose
lac lac
Digestive pathway model
lac lac
RNA When lactose is present, binds
polymerase lac to
lac lac repressor protein & triggers
lac
repressor to release DNA
RNA
induces transcription
TATA repressor
lac
polymerase gene1 gene2 gene3 gene4 DNA
mRNA 1 2 3 4
enzyme1 enzyme2 enzyme3 enzyme4
promoter operator
repressor repressor protein
lac lactose
conformational change in lactose – repressor protein
AP Biology lac repressor
repressor protein! complex
12. Lactose operon
What happens when lactose is present?
Need to make lactose-digesting enzymes
Lactose is allosteric regulator of repressor protein
AP Biology
13. 1961 | 1965
Jacob & Monod: lac Operon
Francois Jacob & Jacques Monod
first to describe operon system
coined the phrase “operon”
AP Biology
Jacques Monod Francois Jacob
14. Operon summary
Repressible operon
usually functions in anabolic pathways
synthesizing end products
when end product is present in excess,
cell allocates resources to other uses
Inducible operon
usually functions in catabolic pathways,
digesting nutrients to simpler molecules
produce enzymes only when nutrient is
available
cell avoids making proteins that have nothing to do,
cell allocates resources to other uses
AP Biology
15. Don’t be repressed!
How can I induce you
to ask Questions?
AP Biology 2007-2008
Notas del editor
Remember: rapid growth generation every ~20 minutes 10 8 (100 million) colony overnight! Anybody that can put more energy to growth & reproduction takes over the toilet. An individual bacterium, locked into the genome that it has inherited, can cope with environmental fluctuations by exerting metabolic control. First, cells vary the number of specific enzyme molecules by regulating gene expression. Second, cells adjust the activity of enzymes already present (for example, by feedback inhibition).