ATagine you tried to cut and paste your gene of imberest into an expr.pdf

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ATagine you tried to cut and paste your gene of imberest into an expression vector for doning tike the figure above using ECoFV restriction enzyme for both ends of your PCR product and your 5... GA T GATC3 yoctor backbene. EoorVis recognition sequence is: 3... CT A T A G... 5 You canfirmed that ligation was successful and you had creular plasmids for transformalion. Ater transfomstion and selecting for bocteria that survived on Ampiciln plates, you find that your colonies do not express your gene of interest. Explan one of the problems with your cloning that may have cauted your experiment to tal..

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Based on the PDF and the video, which of the following statements is the most true about General Mills? Employees are held to high standards of performance and are encouraged to master their current role before trying something new Employees are encouraged to advance their careers in the field that they studied in post-secondary Employees are encouraged to consider a variety of roles and paths in their longterm careers, and join projects that are outside their skill sets. Vertical growth (becoming a manager/people leader) is the most valuable type of growth at General Mills..

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Based on the Ethical Development Framework material covered in this class, the Institute for Global Ethics and Moral Compass.com groups base their assertion that there is a core set of values that cut-across cultures on which of the following? (choose the oNe beit leter? A. Over 20 years of research, including anthropological studies. 6. Nevro science research C. The personal opmons of the leaders of these groups D. Both A and B.

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atsy Manufacturing creates a master budget that includes budgets for each of the responsibility centers of the company using input om the person responsible for each center. Decision-making responsibility is given to each of the managers of their responsibility enters. Manuel is a production supervisor responsible for managing the packaging line that produces organic food bars. Each month. e gets a report that shows the expenditures for his line and is responsible for explaining any major discrepancies. What type of rganizational structure does Eatsy use, and what kind of responsibility center does Manuel manage? Eatsy has a decentralized organizational structure and Manuel manages a cost center. Eatsy has a centralized organizational structure and Manuel manages a revenue center. Eatsy has a decentralized organizational structure and Manuel manages a profit center. Eatsy has a centralized organizational structure and Manuel manages an investment center..

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At your school, the third grade classes have just finished planning and constructing a small vegetable garden on the school grounds. During recess, you observe Oliver and Pandora kneeling at the garden examining the growing plants. Soon each of them begins pulling the plants out of the garden. By the time you reach them, each of them has pulled two plants from the garden plot. How will you respond and why? Use three of the techniques from chapter seven to respond. (ex. I-message, reflective listening, related consequences, or adult guided problem solving).

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Background Julie Martin has completed a preliminary investigation and performed the fact- finding tasks that were described in Chapters 2 and 4 . Now, she will use the results to develop a logical model of the proposed information system. The following scenarios were proposed as possible results of the new system: - Jennifer and Jake Lombard is one of the families that subscribe to OnlineTutors. - They are the parents of two children: Carla is 8 and Michael is 9. - Carla takes a piano lesson from Steve Fabio every Thursday from 4:00pm to 5:00pm - Steve Fabio is a music teacher in an elementary school and tutors several students for Online Tutors. - He can tutor in piano, guitar and violin. - Recently, Michael was struggling with long division. - Jennifer searched through the available math tutors and found that Mark Davis was available right then. - She sent a tutoring request to Mark and Mark sent her a link to a Zoom session. - Mark worked with Michael for 45 minutes on long division. - Mark Davis is a high school math teacher and can tutor in math for all grades K to 12. - The Banks family (John and Helen) has scheduled a French lesson for their two children. - Sandy and Robin three times a week (Monday, Wednesday and Friday) from 1:00 to 2:00 with ... - Madame Thrse Defarge, a tutor who is a native French speaker. - Pat Murphy has scheduled a weekly computer class for her son Bryan who is a high school student. - He will be tutored Monday morning from 9:00am to 10:00am by Peggy Burke who is a computer programmer. - Peggy tutors in computer basics, Microsoft Office, C++, Java and Python. - Recently, Bryan had a question and sent a request for an appointment that afternoon to discuss a C++ program. - Peggy responded that she would meet him at 4:00pm and sent a Zoom link. They talked on Zoom for 30 minutes. In addition each family has an id, an email, and a credit card on file. Each tutor has an id, an email, and bank information to make payments.. Tasks Create a professional looking document to address the issues noted below. Be sure that you have corrected all spelling and grammar errors and all sections of the report are clearly labeled. Use the information given above, and review the previous information given about the proposed system. 1. Prepare a context diagram for the new system. 2. Prepare a diagram 0 DFD for the new system. 3. Create a Data Dictionary to show the data elements, data flows, data stores and processes for the new system using the information in the sample scenarios above. This should match the DFD. Include at least 3 specifications about the format of the data..

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Background You have been hired by the finance minister of small country in central America. The minister Linnea Ribbelov, is a lovely young woman who wants to do the best for the country but her knowledge of economics is poor. She is worried that the election is only few months away. She has hired you a bright graduate from EU Business School. The problem is a steel factory in the country is struggling against cheaper imports from China. Last week she visited the company and they explained that if things do not improve they may have to close some operations which may mean 1000 people lose their jobs. The steel is used by many other industries in the country such boat manufacturing, construction and oil exploration industry as well as others. Some local steel is exported to neighbouring countries. Task You are required to prepare a report: - She is considering putting tariffs on imported steel. Explain with diagram what happens when you put tariffs on steel, given that today it is an open economy. Please show this with diagrams. This should show consumer and producer and explain who are consumers. - Please explain in detail who are winners and loser. She is a politician so must really think of all the winners and losers in the economy- often lots of consequences you don't imagine.- - Think of other creative methods that could be used to help the company or steel industry. However any suggestion must show advantages and disadvantages. There are some ideas in the slides from that class..

Background You have been hired by the finance minister of small count.pdf

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Background Sea otters are one of the few cute and cuddly creatures in the ocean. Visitors to the coast of the Pacific Northwest love to watch their antics as they float effortlessly on their backs among the floating fronds of kelp (large algae) or frolic with one another in play. They also have some human-like skills. Sea otters place rocks on their chests and crack mussels and clams on them, one of the few examples of tool use by animals other than primates. They also roll spiny sea urchins between their paws to make them easier to eat. Part I. Around 1991, Dr. James Estes and his colleagues at the University of Califomia, Santa Cruz, noticed that the otter populations they had been studying for over 20 years were beginning to shrink. Sea otter populations inhabiting several of the Aleutian Islands had declined as much as 90 percent in fewer than 10 years (Figure 1). What could cause such a sharp drop in sea otter numbers in this island chain of Alaska? Alakla (Rtedrume fiom Euse ental, 190t) A. How would you test a bypothesis? Dr. Fstes and his group hypothesired that increased predation by killer whales was the cause of the sea otter decline. This was an unusual idea, since killer whales and sea otters had been observed together in Alaska for decades with no obvious interactions occurring between them. The first time a killer whale was observed attacking a sea otter was in 1991. Nine more attacks were observed in the next seven years and it was these attacks that finally led Dr. Estes and his colleagues to propose their hypothesis. To test this hypothesis, the scientists needed to have infomation about the killer whale. 1. Make a list of the types of information about killer whales: you believe the scientists might need to test their hypothesis that increased predation by the whales was the cause of the sea otter decline. B. What do the data tell you? Estes and his colleagues estimated the impact of killer whales on sea otter populations by comparing trends in population size and survival rates of individually marked otters between two adjacent locations on Adak Island--Clam Lagoon and Kuluk Bay, Kuluk Bay is on an open coast, so sea otters there are exposed to killer whales. In Clam Lagoon, the entrance from the open sea is too narrow and shallow for killer whales to get in. 2. Based on Figures 3 and 4 , what can you conclude about the effects of killer whales on sea otter populations? 3. What level of ecology are the researchers studying? 4. Why do you think the scientists both counted all the sea otters and did the tagging and radio tracking? Why didn't they do just one or the other? 5. What are three abiotic factors in the environment that otters interact with that may affect their survival? 6. What type of ecology experiment is this? (observational, controlled, modeled) Figure 2. Map of the North Pacific Ocean showing the Aleutian Islands and some specific sea otter study sites. (From Estes, J.A., and D.O. Duggins. 1995. "Sea otters a.

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