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hernanlopez1964
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Background Shigella spp. are the primary causative agents of bacillary dysentery. Since its emergence in the late 1980s, the S. flexneri serotype 1c remains poorly understood, particularly with regard to its origin and genetic evolution. This article provides a molecular insight into this novel serotype and the gtrIC gene cluster that determines its unique immune recognition. Results A PCR of the gtrIC cluster showed that serotype 1c isolates from different geographical origins were genetically conserved. An analysis of sequences flanking the gtrIC cluster revealed remnants of a prophage genome, in particular integrase and tRNAPro genes. Meanwhile, Southern blot analyses on serotype 1c, 1a and 1b strains indicated that all the tested serotype 1c strains may have had a common origin that has since remained distinct from the closely related 1a and 1b serotypes. The identification of prophage genes upstream of the gtrIC cluster is consistent with the notion of bacteriophage-mediated integration of the gtrIC cluster into a pre-existing serotype. Conclusions This is the first study to show that serotype 1c isolates from different geographical origins share an identical pattern of genetic arrangement, suggesting that serotype 1c strains may have originated from a single parental strain. Analysis of the sequence around the gtrIC cluster revealed a new site for the integration of the serotype converting phages of S. flexneri. Understanding the origin of new pathogenic serotypes and the molecular basis of serotype conversion in S. flexneri would provide information for developing cross-reactive Shigella vaccines. Keywords Shigella flexneri, Bacillary dysentery, Serotype-conversion , Evolutionary origin, Glucosyltransferase, Serotype 1c
Shigella flexneri serotype 1c derived from serotype 1a by acquisition of gtrI...
Shigella flexneri serotype 1c derived from serotype 1a by acquisition of gtrI...
Swee Seong TANG
ČTÚ-78 995/2015-613
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CTU_cz
Kajian ini akan mengemukakan sejumlah pandangan para ulama terhadap konsep ini agar satu penilaian yang tepat dapat dibuat sama ada untuk menyokong pengamalan konsep Talfiq ataupun tidakmembenarkan sama sekali pengamalannya dalam proses pengeluaran hukum Islam berserta contoh aplikasi penggunaannya dalam isu perbankan Islam.
Aplikasi Talfiq dalam Resolusi Syariah Majlis Penasihat Syariah Bank Negara M...
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Mohd Hafiz Jamaludin
Este vídeo deja claras las ventajas e inconvenientes de contar con uno o varios planes de pensiones para ahorrar con vistas a la jubilación.
¿Ahorrar con uno o varios planes de pensiones?
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List do premiera donalda tuska 2 2 2013
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Prabodh
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drama room
Conformation for drama room for filming
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lillymayb
Background Shigella spp. are the primary causative agents of bacillary dysentery. Since its emergence in the late 1980s, the S. flexneri serotype 1c remains poorly understood, particularly with regard to its origin and genetic evolution. This article provides a molecular insight into this novel serotype and the gtrIC gene cluster that determines its unique immune recognition. Results A PCR of the gtrIC cluster showed that serotype 1c isolates from different geographical origins were genetically conserved. An analysis of sequences flanking the gtrIC cluster revealed remnants of a prophage genome, in particular integrase and tRNAPro genes. Meanwhile, Southern blot analyses on serotype 1c, 1a and 1b strains indicated that all the tested serotype 1c strains may have had a common origin that has since remained distinct from the closely related 1a and 1b serotypes. The identification of prophage genes upstream of the gtrIC cluster is consistent with the notion of bacteriophage-mediated integration of the gtrIC cluster into a pre-existing serotype. Conclusions This is the first study to show that serotype 1c isolates from different geographical origins share an identical pattern of genetic arrangement, suggesting that serotype 1c strains may have originated from a single parental strain. Analysis of the sequence around the gtrIC cluster revealed a new site for the integration of the serotype converting phages of S. flexneri. Understanding the origin of new pathogenic serotypes and the molecular basis of serotype conversion in S. flexneri would provide information for developing cross-reactive Shigella vaccines. Keywords Shigella flexneri, Bacillary dysentery, Serotype-conversion , Evolutionary origin, Glucosyltransferase, Serotype 1c
Shigella flexneri serotype 1c derived from serotype 1a by acquisition of gtrI...
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Odpoved Panu Peterkovi
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Este vídeo deja claras las ventajas e inconvenientes de contar con uno o varios planes de pensiones para ahorrar con vistas a la jubilación.
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