Live Virtual Demo: iNSiGHT DEXA Imaging System Attendees joined us for an interactive live demo of the iNSiGHT DEXA imaging system—a powerful tool for preclinical body composition and bone mineral density analysis. They saw firsthand how it delivered precise and efficient measurements (FAST 25 seconds) to advance their research. What Was Covered: Our expert team guided participants through a hands-on demonstration, including: Accurate fat/lean composition & bone density analysis in mice and ex vivo samples Flexible region-of-interest (ROI) tools for customized analysis User-friendly software that streamlined data acquisition and boosted productivity Key Takeaways: Conducting body composition and bone density analysis with confidence Optimizing workflows with intuitive software tools Simplifying data acquisition without compromising precision Whether attendees were new to DEXA or refining their techniques, this session provided practical insights to enhance their preclinical research.

1. iNSiGHT: Live Virtual
Demonstration
Preclinical DXA system for in
vivo body composition and
bone mineral density
measurements

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Topics of Discussion
• iNSiGHT System Overview
• Hardware components
• Available measurements
• Features and benefits
• Live Virtual Demonstration
• Review of data acquisition, analysis, and export
• Q&A Session
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Dual Energy X-Ray Absorptiometry (DXA or DEXA)
• DXA is used to assess body composition
• Two different x-ray beams, having different energies,
are generated at the source, pass through the body
and hit the x-ray detector
• Different tissues in the body have varying mass
attenuation coefficients (µn)
• Equations are used to determine the tissue type
• Bone
• Soft tissue – fat mass or lean tissue mass
Figure from Luo, Yunhua. 2017. Chapter 3 – Bone Imaging for
Osteoporosis Assessment

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Available Measurements
Parameter
Unit of
Measure
Description
(available on whole animal, or from each ROI)
BMC g
Bone Mineral Contents (Bone Mass)
BMC = bone density x bone area
Fat g Fat mass
Fat Ratio % Fat Ratio = Fat/Total Mass
Lean g Fat free mass
Lean Ratio % Lean Ratio = Lean/Total Mass
Total Mass g Total Mass = Fat + Lean + Bone
BMD g/cm2
Bone Mineral Density
Bone Area cm2
Bone Area in Image
Tissue Area cm2
Tissue Area in Image
Images acquired using the iNSiGHT DXA system

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Acquired Images
Images acquired using the iNSiGHT DXA system
X-Ray Attenuation
Image
Bone Mineral Density
Image
Color Image
Fat (Orange) and Lean (Green) Mass

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iNSiGHT: System Components
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• X-Ray Cabinet
• Self-shielded
• Integrated anesthesia
• Standard electrical connection
• Desktop PC and Insight Software
• Windows 10 operating system
• Ethernet connection (USB WiFi Adapter required)
• Offline Analysis Software
• License-based for offline analysis

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iNSiGHT: System Features and Benefits
• Longitudinal studies
• Non-invasive data acquisition allows studying changes in body
composition over time
• Easy data acquisition
• No preparation steps (other than anesthesia) required
• Low dose radiation
• Minimizing effects on animals during each imaging session
• Fast scan times (<25 seconds/scan)
• Allows for high throughput studies
• High-resolution images (pixel resolution: 100µm at 1.2X, 31µm at 4X)
• Study changes in body and bone composition and on small animals (mice,
rats, etc.)
• Wide scan area (16.5 x 25.5 cm)
• Allows for a variety of animal models to be imaged

8. LIVE
Demonstration of
the iNSiGHT DXA
System

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Q&A Session
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Please feel free to enter your
questions into the Chat or
Q&A function

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Jade Marrow, Ph.D.
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Scintica Instrumentation
jmarrow@scintica.com
Our Global Offices

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