This document discusses Giardia intestinalis, an intestinal parasite that causes diarrhea and malabsorption in humans and other animals. It causes the infection giardiasis. The document then discusses pyroptosis, a form of programmed cell death involved in the innate immune response. There are three pathways of pyroptosis: classical, non-classical, and novel. The purpose of the study is to investigate how the Giardia VSPAS7 protein attenuates Giardia-induced host macrophage pyroptosis. ELISA and western blot were used to detect the presence of the VSPAS7 protein and inflammatory cytokines. Transfection of eukaryotic vectors and immunofluorescence assays were also used. Results are shown

1. S A M U E L D A V I D R I V E R A H I G U I T A
3 R D S E M E S T E R
U . P . B

2. GIARDIA
Giardia intestinalis, also known as G. lamblia
and G. duodenalis, is one of the most common
intestinal parasites in humans and animals such
as sheep and cattle, with the major symptoms
being diarrhea and malabsorption

3. PYROPTOSIS
Pyroptosis, a controlled cell death that is a component of the
host’s innate immune responses, is dependent on the creation of
plasma membrane holes by proteins from the gasder- min
family. Cell swelling, plasma membrane rupture, chromatin
breakage and the release of inner pro-inflam- matory substances
are its distinguishing features. There are three pyroptosis
pathways:
The classical
The non-classical
The novel pathway

4. PURPOSE
How the Giardia VSPAS7 protein attenuates
Giardia intestinalis-induced host macrophage
pyroptosis

5. METODOS
ELISA
Western Blot
Se utilizó para detectar la
presencia de la proteina
GiardiaVSPAS7
Se utilizo para detectar la presencia de
sustancias especificas: como células
supernadantes y secrecion de citoquinas
proinflamatorias

6. METODOS
Transfección de vectores
eucariotas
La transfección con vectores eucarióticos es
un proceso en el que se introducen genes o
segmentos de ADN en células eucariotas
Ensayos de
inmunofluorescencia
Se utilizo para identificar y visualizar
la localización subcelular de VSPAS7

7. RESULTADOS
Figura 1

8. RESULTADOS
Figura 3 Figura 4

9. DISCUSION
Author What it say? Article
Maayeh SY, Liu J, Peirasmaki
D, Hörnaeus K, etc (2017)
“Characterization of the
Giardia intestinalis secretome
during interaction with human
intestinal epithelial cells”
Chen X, Liu G, Yuan Y, Wu
G, Wang S, Yuan L.
(2019)
“NEK7 interacts with NLRP3
to modulate the pyroptosis in
inflammatory bowel disease”
He W, Hu Z, Zhong Y, Wu
C, Li J.
(2023)
“The Potential of NLRP3
inflamma- some as a
therapeutic target in
neurological diseases”

10. Research enables the
advancement of personalized
medicine approaches. By
analyzing individual genetic
variations, gene expression
patterns, and molecular
profiles.
CONCLUSION
Drives biotechnological
innovations that have
applications beyond medicine.
These innovations contribute
to addressing global
challenges