Seminario biología molecular, Maria Antonia Maya. (2).pdf
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This document summarizes a study on the role of spermidine in Salmonella pathogenesis. The main objective was to discover the mechanism by which spermidine assists in the early stages of Salmonella pathogenesis. Chromosomal knockout strains were created and evaluated for gene expression, adhesion to intestinal cells, and presence of flagellin and regulatory proteins on the bacterial surface with and without spermidine supplementation. The results helped identify key genes, proteins, and structures involved in Salmonella virulence and understand the importance of spermidine in bacterial motility and virulence.
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Seminario biología molecular Maria Antonia Maya. (3).pdf
This document summarizes a study on the role of spermidine, a polyamine, in Salmonella Typhimurium virulence and motility. The main objective was to discover the mechanism by which spermidine assists in early Salmonella pathogenesis. Methods included generating chromosomal knockout strains of genes involved in spermidine transport and biosynthesis. Immunofluorescence, qRT-PCR, and immunoblotting were used to evaluate the expression of flagellin, fimbrial proteins, and regulatory proteins with and without spermidine supplementation. Results showed spermidine increased adhesion to intestinal cells and expression of motility proteins. The study concluded spermidine is important for Salmonella mot
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This document summarizes a study on the role of spermidine, a polyamine, in Salmonella Typhimurium virulence and motility. The main objective was to discover the mechanism by which spermidine assists in early Salmonella pathogenesis. Methods included generating chromosomal knockout strains of genes involved in spermidine transport and biosynthesis. Immunofluorescence, qRT-PCR, and immunoblotting were used to evaluate the expression of flagellin, fimbrial proteins, and regulatory proteins with and without spermidine supplementation. Results showed spermidine increased adhesion to intestinal cells and expression of motility proteins. The study concluded spermidine is important for Salmonella mot
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1. Salmonella is a major cause of foodborne illness worldwide. It can cause gastroenteritis through ingestion of contaminated food or water.
2. When Salmonella is ingested, it is able to penetrate the epithelial cells of the intestine and migrate to the lamina propria, multiplying in lymphoid follicles and causing inflammation. This stimulates fluid secretion and diarrhea.
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Seminario biología molecular Maria Antonia Maya. (3).pdf
This document summarizes a study on the role of spermidine, a polyamine, in Salmonella Typhimurium virulence and motility. The main objective was to discover the mechanism by which spermidine assists in early Salmonella pathogenesis. Methods included generating chromosomal knockout strains of genes involved in spermidine transport and biosynthesis. Immunofluorescence, qRT-PCR, and immunoblotting were used to evaluate the expression of flagellin, fimbrial proteins, and regulatory proteins with and without spermidine supplementation. Results showed spermidine increased adhesion to intestinal cells and expression of motility proteins. The study concluded spermidine is important for Salmonella mot
Seminario biología molecular Maria Antonia Maya. (3).pdf
This document summarizes a study on the role of spermidine, a polyamine, in Salmonella Typhimurium virulence and motility. The main objective was to discover the mechanism by which spermidine assists in early Salmonella pathogenesis. Methods included generating chromosomal knockout strains of genes involved in spermidine transport and biosynthesis. Immunofluorescence, qRT-PCR, and immunoblotting were used to evaluate the expression of flagellin, fimbrial proteins, and regulatory proteins with and without spermidine supplementation. Results showed spermidine increased adhesion to intestinal cells and expression of motility proteins. The study concluded spermidine is important for Salmonella mot
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1. Salmonella is a major cause of foodborne illness worldwide. It can cause gastroenteritis through ingestion of contaminated food or water.
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3. Laboratory diagnosis of Salmonella infection involves culturing specimens from foods, stool, vomit or organs in nutrient media and examining the isolates for their cultural, serological and biological properties to identify the Salmonella species.
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Copyright © 2016 Elsevier Inc. All rights reserved.
Se recalca que esta presentación no es mas que una explicación de el articulo hecho por los autores mencionados
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Proteus mirabilis is a gram-negative bacteria commonly found in the human intestinal tract. It can cause urinary tract infections when it enters the urinary system, where it produces an enzyme called urease that breaks down urea into ammonia, raising the pH of urine. P. mirabilis is responsible for 90% of Proteus infections and can lead to sepsis, abdominal infections, and secondary infections of wounds and ears. It is diagnosed through laboratory tests detecting its unique swarming motility, inability to metabolize lactose, and distinctive fishy odor. Treatment involves antibiotics like beta-lactams, aminoglycosides, and fluoroquinolones to which P. mirabilis
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- 1. Maria Antonia Maya Ardila Tercer semestre Universidad Pontificia Bolivariana
- 2. INTRODUCTION: Salmonella Typhimurium Salmonella enterica is a gram- negative bacillus pathogenic species, which is ingested through contaminated food and water. It usually causes high fever, abdominal pain, nausea, diarrhea and vomiting.
- 3. INTRODUCTION: Spermidine: Spermidine is a poly-cationic and polyamine that participates in the virulence and motility of bacterias. Salmonella regulates spermidine transport and biosynthesis processes.
- 4. MAIN OBJECTIVE: “The main objective of this study is to discover the mechanism by which spermidine assists in the early stages of Salmonella pathogenesis”.
- 5. BACTERIAL GENE KNOCKOUT AND STRAIN GENERATION 1. 2. IMMUNOFLUORESCENCE MATERIAL AND METHODS: There were created chromosomal knockout strains: STM ΔpotCD, STM ΔspeED, STM ΔpotCDΔspeED. The immunofluorescence was used to evaluate the expression of flagellin in the surface of Salmonella Typhimurium.
- 6. MATHERIAL AND METHODS 3. RNA ISOLATION AND QRT-PCR 4. IMMUNOBLOTTING Thanks to the immonublot they identified fimbrial proteins and regulatory proteins like FliA and GroEL in Salmonella Typhimurium surface. There were evaluated the gene expression levels of interest, like the ATP-dependent transport apparatus genes potA, potB, potC, and potD.
- 7. RESULTS: Mice intestine (Peyer patches). Adhesion to Caco 2 cells. a. b.
- 8. Expression of FliC in the surface. c. d. Evaluation of FliA and GroEL with and without spermidine supplementation
- 9. DISCUSSION:
- 10. CONCLUSIONS: Molecular biology was really useful to identify how the process of Salmonella virulence works, as it let us identify the key genes, proteins and structures that are involved in this process. This investigation was really useful to understand the importance of spermidine in eukaryotic and prokaryotic cells, as it let us know about the importance of spermidine in the motility and virulence of bacterias like Salmonella.