This document summarizes a study on the role of spermidine, a polyamine, in Salmonella Typhimurium virulence and motility. The main objective was to discover the mechanism by which spermidine assists in early Salmonella pathogenesis. Methods included generating chromosomal knockout strains of genes involved in spermidine transport and biosynthesis. Immunofluorescence, qRT-PCR, and immunoblotting were used to evaluate the expression of flagellin, fimbrial proteins, and regulatory proteins with and without spermidine supplementation. Results showed spermidine increased adhesion to intestinal cells and expression of motility proteins. The study concluded spermidine is important for Salmonella mot