This document summarizes a study that aimed to identify how the Chlamydia trachomatis type III secretion system effector protein CT622 induces proinflammatory cytokines through TLR2/TLR4-mediated MAPK/NF-κB pathways in THP-1 cells. The study used techniques like ELISA, western blot, immunoflorescence, and RNA interference to examine the expression of CT622 and proteins in the MAPK/NF-κB inflammatory signaling pathways. The results supported that CT622 activates these pathways through TLR2 and TLR4 to regulate the secretion of inflammatory mediators. The discussion concluded that molecular biology techniques provide insights into specific molecular targets and signaling pathways important for developing targeted therapies for C. Trachomat

1. UPB
THIRD SEMESTER OF MEDICINE
Por: Sara Marcela Díaz R.

2. Chlamydia trachomatis
Introduction
It’s a gram- negative bacterium that causes the
sexually transmitted infection (STI) known as
chlamydia.
is associated with the induction of the host
inflammatory response, some of it’s
consequences include:
Infertility Prostatitis Ectopic pregnancies

3. MAPK; NF-κB :signaling pathway
T3SS and CT622
MAPK (Mitogen-Activated Protein Kinase) and NF-κB (Nuclear Factor-kappa B) are
signaling pathways involved in cellular responses. In the context of chlamydia
infection, MAPK and NF-κB pathways are often activated as part of the host cell's
response to the presence of the bacteria .
T3SS (Type III secretion system)
CT622: Effector protein
THP-1: Monocytic cell line used in immunology
studies

4. Main Objective
Identifying how in Chlamydia trachomatis T3SS
Effector CT622 induces Proinflammatory
Cytokines Through TLR2/TLR4-
Mediated MAPK/NF-κB Pathways in THP-1 Cells.

5. Methods
ELISA: Enzyme-Linked Immunosorbent Assay
Western blot Biochemical technique using gel
electrophoresis to know the
expression and quantification of
a specific protein in a biological
sample. such as CT622.
Antigen/ antibody reaction to determine the
presence of a protein, such as the proteins in
the inflammatory signaling pathways
(MAPK/NF-κB)

6. The use of fluorescently labeled antibodies
to detect and visualize specific proteins or
other molecules within cells or tissues.
Immunoflorecence
Methods
RNA interference
Involves inhibiting the expression of specific
genes through the introduction of small RNA
molecules.
For TLR2 and TLR4
For p65 localization

7. Figure 3
Results
Figure 2

8. Results
Figure 4

9. Author What did they say? Agree or disagree
Rodrígues. et al (2022)
“Severe sequelae induced by C
trachomatis are often mediated
by harmful immune responses
after recurrent or chronic infec-
tion, including blindness, ectopic
pregnancy, and infertility”
Agree
O’Connell CM. et al (2006)
“TLR2 and TLR4 are involved in
the initiation of immune cell
activation and the immune
cascade response following
chlamydial infection, particularly
in the ex- pression of cytokines
and chemokines”
Agree
McGuire VA, Arthur JS (2015)
“MAPK and NF-κB are the core
downstream signaling pathways
of TLRs to regulate the secretion
of inflammatory mediators”
Agree
DISCUSSION

10. The techniques used in molecular biology help identify specific
molecular targets, such as CT622 or signaling pathways like NF-κB
and MAPK. This knowledge is essential for developing targeted
therapies.
1.
Molecular biology goes hand in hand with medicine by providing the
detailed molecular insights for a deeper understanding of the
Inflammatory mechanism of C. Trachomatis
2.
Conclusions