Seminario biologia molecular universidad pontificia bolivariana
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This study investigated whether Angiotensin-(1-7) can mitigate inflammation in lipopolysaccharide (LPS)-induced macrophages and septic mice by regulating the Warburg effect, a metabolic process where cancer cells rely on glycolysis rather than oxidative phosphorylation. The study used techniques like western blot, ELISA, qRT-PCR, and immunofluorescence staining to examine levels of inflammatory cytokines and proteins involved in the Warburg effect. Preliminary results found that Angiotensin-(1-7) reduced inflammation and modulated the Warburg effect. The conclusions were that Angiotensin-(1-7) shows promise in mitigating sepsis-related inflammation by targeting cellular metabolic pathways like the Warburg effect.
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Npy final paper
This study investigated the effects of neuropeptide Y (NPY) on signaling proteins in C. elegans. NPY is a neurotransmitter that regulates anxiety in humans. The researchers extracted proteins from C. elegans exposed to NPY and analyzed them using ELISA. They found a statistically significant upregulation of Akt1 protein levels in the presence of NPY, but no significant effects on other proteins like p38 MAP kinase and Stat3. This suggests NPY may activate Akt1 signaling in C. elegans. The results help validate the proposed pathway of NPY's anxiolytic effects.
Npy
This study investigated the effects of neuropeptide Y (NPY) on signaling proteins in C. elegans. NPY is a neurotransmitter that regulates anxiety in humans. The researchers extracted proteins from C. elegans exposed to NPY and analyzed them using ELISA. They found a statistically significant upregulation of Akt1 protein levels in the presence of NPY, but no significant effects on other proteins like p38 MAP kinase and Stat3. This suggests NPY specifically increases Akt1, a regulator of cell growth and survival, in C. elegans. The results help validate the proposed pathway of NPY's anxiolytic effects.
Npy final paper
This study investigated the effects of neuropeptide Y (NPY) on signaling proteins in C. elegans. NPY is a neurotransmitter that regulates anxiety in humans. The researchers extracted proteins from C. elegans exposed to NPY and analyzed them using ELISA. They found a statistically significant upregulation of Akt1 protein levels in the presence of NPY, but no significant effects on other proteins like p38 MAP kinase and Stat3. This suggests NPY may upregulate Akt1 through a CRE element in its gene. Further experiments are needed to validate the proposed signaling pathway and determine if NPY increases phospho-CREB levels.
Gasdermin D Open Sepsis-Induced Acute Kidney Injury via Cell Pyroptosis by NL...
Gasdermin D Open Sepsis-Induced Acute Kidney Injury via Cell Pyroptosis by NL...ANALYTICAL AND QUANTITATIVE CYTOPATHOLOGY AND HISTOPATHOLOGY
1) The study investigated the effects of gasdermin D on pyroptosis in a mouse model of sepsis-induced acute kidney injury.
2) The results showed that gasdermin D expression was increased in mice with sepsis-induced acute kidney injury and promoted inflammation and pyroptosis in kidney cells.
3) Downregulating gasdermin D decreased inflammation and pyroptosis, and the NLRP3 inflammasome was identified as an important target of gasdermin D in mediating inflammation during sepsis-induced acute kidney injury.ApoB Sequencing
This document describes experiments performed to sequence the human Apolipoprotein B (ApoB) gene. A portion of the ApoB gene was amplified via PCR and subcloned into E. coli plasmid vectors. The plasmid vectors containing the inserted ApoB fragment were then purified and sequenced. Sequence analysis revealed that human ApoB is highly similar to Canis lupus familiaris (dog) ApoB, indicating evolutionary conservation. The experiments aimed to accurately insert, track, and sequence the ApoB gene to better understand its structure, function, and evolutionary relationships.
Sfnge array poster
This study examined how chronic physical restraint stress alters gene expression in mouse spleens. Mice were subjected to restraint stress for 12 hours per day for two days. Microarray analysis found that 48 out of 225 apoptosis- and p53 signaling-related genes showed significant expression changes in stressed mice compared to unstressed controls. Four genes (Fas, FADD, p53, p21) were validated with real-time PCR. The results provide evidence that apoptotic pathways contribute to stress-induced lymphopenia and identify potential stress response biomarkers.
Murphy et al, AJP Cell Physiol 296_ C746–C756, 2009.
Fasting enhances the response of hypothalamic arcuate nucleus neuropeptide Y (NPY) neurons that are inhibited by glucose (NPY-GI neurons) to decreases in extracellular glucose. Using hypothalamic explants from fed and fasted rats, the authors showed that fasting enhanced NPY release in response to lowered glucose concentrations. They also demonstrated that fasting caused NPY-GI neurons to depolarize, or become more electrically active, in response to smaller decreases in glucose levels. This increased responsiveness was mediated by the cellular energy sensor AMPK. During fasting, decreased leptin and glucose activate AMPK in NPY-GI neurons, making them more sensitive to fluctuations in glucose.
Seminario Biología Molecular Manuela Álvarez Ramírez
This document discusses alveolar hypercoagulation, a pathologic state of exaggerated coagulation within the lungs. It can be associated with conditions like respiratory distress syndrome, pneumonia, and pulmonary embolism. The document introduces the RUNX1/NF-kB signaling pathway, which regulates immune responses, hypercoagulation, and fibrinolytic inhibition. It describes methods used like immunohistochemistry, ELISA, western blot, and qPCR to analyze markers in lung tissue and determine the effects of 6-gingerol on inflammation pathways. The results and discussion sections are not included in full. The conclusions emphasize how molecular biology has improved diagnostic tools and contributed to more personalized medicine through targeted therapies based on molecular profiles.
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Npy final paper
This study investigated the effects of neuropeptide Y (NPY) on signaling proteins in C. elegans. NPY is a neurotransmitter that regulates anxiety in humans. The researchers extracted proteins from C. elegans exposed to NPY and analyzed them using ELISA. They found a statistically significant upregulation of Akt1 protein levels in the presence of NPY, but no significant effects on other proteins like p38 MAP kinase and Stat3. This suggests NPY may activate Akt1 signaling in C. elegans. The results help validate the proposed pathway of NPY's anxiolytic effects.
Npy
This study investigated the effects of neuropeptide Y (NPY) on signaling proteins in C. elegans. NPY is a neurotransmitter that regulates anxiety in humans. The researchers extracted proteins from C. elegans exposed to NPY and analyzed them using ELISA. They found a statistically significant upregulation of Akt1 protein levels in the presence of NPY, but no significant effects on other proteins like p38 MAP kinase and Stat3. This suggests NPY specifically increases Akt1, a regulator of cell growth and survival, in C. elegans. The results help validate the proposed pathway of NPY's anxiolytic effects.
Npy final paper
This study investigated the effects of neuropeptide Y (NPY) on signaling proteins in C. elegans. NPY is a neurotransmitter that regulates anxiety in humans. The researchers extracted proteins from C. elegans exposed to NPY and analyzed them using ELISA. They found a statistically significant upregulation of Akt1 protein levels in the presence of NPY, but no significant effects on other proteins like p38 MAP kinase and Stat3. This suggests NPY may upregulate Akt1 through a CRE element in its gene. Further experiments are needed to validate the proposed signaling pathway and determine if NPY increases phospho-CREB levels.
Gasdermin D Open Sepsis-Induced Acute Kidney Injury via Cell Pyroptosis by NL...
Gasdermin D Open Sepsis-Induced Acute Kidney Injury via Cell Pyroptosis by NL...ANALYTICAL AND QUANTITATIVE CYTOPATHOLOGY AND HISTOPATHOLOGY
1) The study investigated the effects of gasdermin D on pyroptosis in a mouse model of sepsis-induced acute kidney injury.
2) The results showed that gasdermin D expression was increased in mice with sepsis-induced acute kidney injury and promoted inflammation and pyroptosis in kidney cells.
3) Downregulating gasdermin D decreased inflammation and pyroptosis, and the NLRP3 inflammasome was identified as an important target of gasdermin D in mediating inflammation during sepsis-induced acute kidney injury.ApoB Sequencing
This document describes experiments performed to sequence the human Apolipoprotein B (ApoB) gene. A portion of the ApoB gene was amplified via PCR and subcloned into E. coli plasmid vectors. The plasmid vectors containing the inserted ApoB fragment were then purified and sequenced. Sequence analysis revealed that human ApoB is highly similar to Canis lupus familiaris (dog) ApoB, indicating evolutionary conservation. The experiments aimed to accurately insert, track, and sequence the ApoB gene to better understand its structure, function, and evolutionary relationships.
Sfnge array poster
This study examined how chronic physical restraint stress alters gene expression in mouse spleens. Mice were subjected to restraint stress for 12 hours per day for two days. Microarray analysis found that 48 out of 225 apoptosis- and p53 signaling-related genes showed significant expression changes in stressed mice compared to unstressed controls. Four genes (Fas, FADD, p53, p21) were validated with real-time PCR. The results provide evidence that apoptotic pathways contribute to stress-induced lymphopenia and identify potential stress response biomarkers.
Murphy et al, AJP Cell Physiol 296_ C746–C756, 2009.
Fasting enhances the response of hypothalamic arcuate nucleus neuropeptide Y (NPY) neurons that are inhibited by glucose (NPY-GI neurons) to decreases in extracellular glucose. Using hypothalamic explants from fed and fasted rats, the authors showed that fasting enhanced NPY release in response to lowered glucose concentrations. They also demonstrated that fasting caused NPY-GI neurons to depolarize, or become more electrically active, in response to smaller decreases in glucose levels. This increased responsiveness was mediated by the cellular energy sensor AMPK. During fasting, decreased leptin and glucose activate AMPK in NPY-GI neurons, making them more sensitive to fluctuations in glucose.
Seminario Biología Molecular Manuela Álvarez Ramírez
This document discusses alveolar hypercoagulation, a pathologic state of exaggerated coagulation within the lungs. It can be associated with conditions like respiratory distress syndrome, pneumonia, and pulmonary embolism. The document introduces the RUNX1/NF-kB signaling pathway, which regulates immune responses, hypercoagulation, and fibrinolytic inhibition. It describes methods used like immunohistochemistry, ELISA, western blot, and qPCR to analyze markers in lung tissue and determine the effects of 6-gingerol on inflammation pathways. The results and discussion sections are not included in full. The conclusions emphasize how molecular biology has improved diagnostic tools and contributed to more personalized medicine through targeted therapies based on molecular profiles.
Seminario molecular[1] listo
This study explores how aristolochic acid (AA) affects leptin production in kidney fibroblasts. The researchers found that AA treatment increased leptin secretion and expression in kidney fibroblasts in a time and dose-dependent manner. AA was found to activate C/EBPα and the PI3K/Akt pathway to upregulate leptin expression. Knocking down C/EBPα or inhibiting the PI3K/Akt pathway blocked AA-induced leptin expression, demonstrating these factors are involved in AA's effects on leptin. Overall, the results suggest AA promotes renal fibrosis by stimulating leptin production in fibroblasts through C/EBPα and PI3K/Akt signaling
Kwon et al 2007 jbs
Transcription factors of the nuclear factor κ B family are the paradigm for signaling dependent nuclear translocation and are ideally suited to analysis through image-based chemical genetic screening. The authors describe combining high-content image analysis with a compound screen to identify compounds affecting either nuclear import or export. Validation in silico and in vitro determined an EC50 for the nuclear export blocker leptomycin B of 2.4 ng/mL (4.4 nM). The method demonstrated high selectivity (Z′ >0.95), speed, and robustness in a screen of a compound collection. It identified the IκB protein kinase inhibitor BAY 11 7082 as an import inhibitor, the p38 mitogen-activated protein (MAP) kinase inhibitor PD98509 as an import enhancer, and phorbol ester as an export inhibitor. The results establish a robust method for identifying compounds regulating nucleocy- toplasmic import or export and also implicate MAP kinases in nuclear import of nuclear factor κ B
ResearchDayPosterSpring2016
1) CCR1 signaling in macrophages promotes glioblastoma invasion by activating the JNK/AP1 pathway, and this study tests whether inhibiting CCR1 with JLY-133 blocks this pathway.
2) A dual luciferase reporter assay showed that co-culturing glioblastoma cells with microglia increased AP-1 luciferase activity, and this was reduced when JLY-133 was added.
3) Western blotting also demonstrated increased phosphorylated JNK and cJun from co-culture, indicating JNK/AP1 pathway activation, which was inhibited by JLY-133 treatment.
NGUYENTHINHI-W9.pptx
1. The document reports on a study investigating the role of insulin signaling in pancreatic β cells using Cre-loxP mediated recombination to generate βIRKO mice lacking the insulin receptor in β cells.
2. Results showed βIRKO mice exhibited impaired glucose tolerance and insulin secretion compared to controls. Expression of genes CENP-A and PLK1 were decreased in βIRKO cells.
3. Further experiments demonstrated insulin stimulates FoxM1 binding to the CENP-A and PLK1 genes, and CENP-A expression is reduced in type 2 diabetes patient islets. Knockout of CENP-A in β cells decreased proliferation.
N fk b signaling in cancer
This document summarizes research on NF-kB signaling in cancer, specifically gastric cancer. It describes the background, structure and activation processes of NF-kB. Experiments show that NF-kB1 and RELA are upregulated in gastric cancer cell lines and tumors, and their knockdown inhibits tumor growth in vitro and in vivo. Furthermore, miR-508-3p is identified as a tumor suppressor that directly targets and downregulates NF-kB1 in gastric cancer. Re-expression of NF-kB1 partly reverses the tumor suppressive effects of miR-508-3p overexpression. In conclusion, downregulation of miR-508-3p contributes to canonical NF-kB activation in gastric tumorigenesis.
Induced Expression of CYP1A by Triclosan in Zebra Fish to measure toxicologic...
1) The study examines the effects of triclosan exposure on zebrafish by observing changes in expression of the CYP1A and TCF3A genes.
2) Using qPCR, the study found a 250% increase in CYP1A expression in triclosan-treated zebrafish compared to controls, indicating triclosan exposure induces CYP1A expression. However, a 9% increase in TCF3A expression in treated fish was not statistically significant.
3) EROD assays were also used to measure CYP1A activity, showing triclosan exposure induces CYP1A enzyme activity in zebrafish. The study suggests further research is
Darley Source Poster final
- Dopamine plays an important role in motor control and Parkinson's disease is characterized by degeneration of dopaminergic neurons.
- Previous models of Parkinson's disease in C. elegans observed neuronal degeneration via fluorescent microscopy at day 9 of development, which was time-consuming.
- This study developed a new assay to detect behavioral defects in C. elegans correlated with dopamine neuron degeneration at an earlier time point (day 3), allowing faster screening of factors that contribute to Parkinson's disease onset.
Breast final
This document summarizes the development of novel inhibitors of PAK1 for the treatment of breast cancer. It outlines assays to identify small molecules that promote PAK1 dimerization or stabilize its autoinhibited state. Hits from cytotoxicity screening will undergo PAK1 activity, dimerization, and phosphorylation assays. Promising leads will be characterized in vitro and tested in a mouse efficacy model. The goal is to develop PAK1 inhibitors with improved specificity and efficacy compared to existing drugs.
48x36_horizontal7_23_14
This study aimed to investigate mechanisms regulating expression of the tumor suppressor DAPK1 in chronic lymphocytic leukemia (CLL) cells. The researchers found that ZIPK, an interacting partner of the transcription factor ATF6, plays a critical role in IFN-γ-induced autophagy by promoting DAPK1 expression. Knockdown of ZIPK in cells prevented autophagy stimulation by IFN-γ. Additionally, primary CLL cells showed low levels of transcripts for proteins involved in autophagy induction like C/EBP-β, ATF6, and DAPK1, suggesting dysregulation of the pathway. Understanding how DAPK1 expression is controlled could help develop therapies
Seminario biomol
The document discusses the assembly and antitumor activity of lycium barbarum polysaccharide-platinum conjugates. Specifically, it investigates conjugating lycium barbarum polysaccharide (LBP) extracted from goji berries with 5-aminosalicylic acid (5-ASA) and platinum to form LBP-5-ASA-Pt conjugates. The cytotoxicity of the conjugates was tested on several cancer cell lines and a normal kidney cell line. Results showed the conjugates were cytotoxic to cancer cells, with LBP-5-ASA-Pt having the strongest effect. Further experiments showed the conjugates interacted with DNA and induced cell cycle arrest and apoptosis in cancer cells.
PERK PowerPoint
This document describes a study examining the role of the unfolded protein response (UPR) pathway protein PERK in Ebolavirus replication. The researchers generated VeroE6 cell lines with reduced PERK expression using lentiviral transduction of shRNA against PERK. They found that PERK knockdown cells had lower PERK and phosphorylated PERK levels both at baseline and during ER stress induction. These cells were then infected with Ebolavirus, and a plaque assay found that PERK knockdown cells produced significantly fewer viral plaques over time compared to control cells, suggesting PERK promotes Ebolavirus replication efficiency.
Seahorse Poster DOS (JJW Edits 6-15-15)
The study compared measurements of oxygen consumption rate (OCR) in pancreatic islets from pigs and non-human primates using an extracellular flux analyzer and a stirred microchamber method. The analyzer produced comparable OCR measurements to the standard method but with higher variability. Basal OCR was lower in non-human primate islets than pig islets, but primate islets showed a greater increase in OCR in response to hyperglycemia. Immature pig islets had almost no OCR response to glucose. The analyzer can provide species-specific and maturation state insights into islet function and mitochondrial health beyond what the standard method allows.
Bioinformatic data analysis – comparison from three human studies using diffe...
This document summarizes three bioinformatics studies comparing gene expression data from human samples using different Affymetrix platforms: 1) comparing periodontal ligament and gingival cells, 2) identifying genes regulated by nuclear IGFBP5 in osteoblasts, and 3) effects of LPS on osteoblasts. Key findings include identification of differentially expressed genes and biological processes between tissue types in study 1, and processes related to cell cycle, proliferation and splicing regulated by IGFBP5 in study 2. Study 3 on LPS effects found upregulation of factors stimulating osteoclasts and downregulation of processes like mitosis and cell cycle. The document discusses analysis tools and validation of array results.
Bio390 final paper
This document describes site-directed mutagenesis experiments performed on the global transcription factor cAMP receptor protein (CRP) in Escherichia coli. Specifically, lysine residue 36 of CRP was mutated to alanine using site-directed mutagenesis to investigate how protein acetylation may affect CRP function and gene regulation. The document provides background on CRP and its role in transcription, as well as an overview of protein acetylation and its effects. It then describes the methods used to mutate lysine 36 to alanine in CRP and plans to analyze the mutant protein.
Research Symposium Poster (Spring - April 2014)
This study aimed to assess the anti-inflammatory potential of 5,6-EET-EA. Researchers isolated splenocytes from mice and stimulated them with LPS to induce inflammation. They then treated the splenocytes with 5,6-EET-EA or anandamide (AEA) and used flow cytometry to detect levels of inflammatory cytokines IL-6 and TNF-α. The results showed that 5,6-EET-EA decreased IL-6 levels at certain concentrations, similar to the effects of AEA. This suggests that 5,6-EET-EA has anti-inflammatory properties by reducing the levels of pro-inflammatory cytokines. More research is needed to confirm these anti-inflammatory effects, including
44-150724PST_Peptalk_2013_AlphaLISA_immunoassays
The document describes the development and use of AlphaLISA assays to characterize monoclonal antibody biotherapeutics. AlphaLISA is a homogeneous bead-based immunoassay that provides advantages over ELISA, including shorter assay times without washing steps. New AlphaLISA assays have been developed for detecting fucosylated IgG, host cell proteins, and quantifying therapeutic antibodies. Data shows the assays offer sensitive and precise detection of analytes during biotherapeutic development and characterization.
Participation of the gabaergic system on the glutamate
This study investigated the participation of the GABAergic system in regulating glutamate release from frontal cortex synaptosomes in a rat model of experimental autoimmune encephalomyelitis (EAE), which mimics multiple sclerosis in humans. The results showed reduced GABAergic inhibition of glutamate release and impaired GABA regulation of synapsin I phosphorylation in synaptosomes from EAE rats compared to controls. Specifically, GABA had no effect on glutamate release or synapsin I phosphorylation in EAE rats, suggesting alterations in the GABAergic system may contribute to cortical pathology in EAE.
Jianying Xiao CV 2016
Jianying Xiao has over 16 years of experience in pharmaceutical research. She has expertise in in vivo and in vitro drug discovery techniques related to drug metabolism, infectious diseases, immunology, and cardio-metabolic disorders. She is proficient in various research techniques including animal handling, molecular biology, cell culture, and data analysis software. Jianying has worked at Merck & Co. for over 18 years, leading numerous projects that resulted in publications, patents, and awards. Her work has advanced drug programs from research through clinical trials.
Advantages And Disadvantages Of Reverse Sequence Syphilis...
Here are the key conclusions I can draw from the results of this experiment:
- The chemotherapeutic drug was effective at reducing cell viability in a dose-dependent manner across all three cancer cell lines tested (HEPG2, PANC-1, PC-3). Higher drug concentrations led to lower percentages of viable cells.
- PANC-1 and PC-3 cell lines appeared to be more sensitive to the drug, as viability decreased more sharply with increasing concentration compared to HEPG2. This suggests the drug may be more effective against pancreatic and prostate cancers.
- Even at the highest concentration of 40μM, the drug did not reduce HEPG2 viability below 60%. This indicates HEPG2 may be more
International Journal of Pharmaceutical Science Invention (IJPSI)
International Journal of Pharmaceutical Science Invention (IJPSI) is an international journal intended for professionals and researchers in all fields of Pahrmaceutical Science. IJPSI publishes research articles and reviews within the whole field Pharmacy and Pharmaceutical Science, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online
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Similar a Seminario biologia molecular universidad pontificia bolivariana
Seminario molecular[1] listo
This study explores how aristolochic acid (AA) affects leptin production in kidney fibroblasts. The researchers found that AA treatment increased leptin secretion and expression in kidney fibroblasts in a time and dose-dependent manner. AA was found to activate C/EBPα and the PI3K/Akt pathway to upregulate leptin expression. Knocking down C/EBPα or inhibiting the PI3K/Akt pathway blocked AA-induced leptin expression, demonstrating these factors are involved in AA's effects on leptin. Overall, the results suggest AA promotes renal fibrosis by stimulating leptin production in fibroblasts through C/EBPα and PI3K/Akt signaling
Kwon et al 2007 jbs
Transcription factors of the nuclear factor κ B family are the paradigm for signaling dependent nuclear translocation and are ideally suited to analysis through image-based chemical genetic screening. The authors describe combining high-content image analysis with a compound screen to identify compounds affecting either nuclear import or export. Validation in silico and in vitro determined an EC50 for the nuclear export blocker leptomycin B of 2.4 ng/mL (4.4 nM). The method demonstrated high selectivity (Z′ >0.95), speed, and robustness in a screen of a compound collection. It identified the IκB protein kinase inhibitor BAY 11 7082 as an import inhibitor, the p38 mitogen-activated protein (MAP) kinase inhibitor PD98509 as an import enhancer, and phorbol ester as an export inhibitor. The results establish a robust method for identifying compounds regulating nucleocy- toplasmic import or export and also implicate MAP kinases in nuclear import of nuclear factor κ B
ResearchDayPosterSpring2016
1) CCR1 signaling in macrophages promotes glioblastoma invasion by activating the JNK/AP1 pathway, and this study tests whether inhibiting CCR1 with JLY-133 blocks this pathway.
2) A dual luciferase reporter assay showed that co-culturing glioblastoma cells with microglia increased AP-1 luciferase activity, and this was reduced when JLY-133 was added.
3) Western blotting also demonstrated increased phosphorylated JNK and cJun from co-culture, indicating JNK/AP1 pathway activation, which was inhibited by JLY-133 treatment.
NGUYENTHINHI-W9.pptx
1. The document reports on a study investigating the role of insulin signaling in pancreatic β cells using Cre-loxP mediated recombination to generate βIRKO mice lacking the insulin receptor in β cells.
2. Results showed βIRKO mice exhibited impaired glucose tolerance and insulin secretion compared to controls. Expression of genes CENP-A and PLK1 were decreased in βIRKO cells.
3. Further experiments demonstrated insulin stimulates FoxM1 binding to the CENP-A and PLK1 genes, and CENP-A expression is reduced in type 2 diabetes patient islets. Knockout of CENP-A in β cells decreased proliferation.
N fk b signaling in cancer
This document summarizes research on NF-kB signaling in cancer, specifically gastric cancer. It describes the background, structure and activation processes of NF-kB. Experiments show that NF-kB1 and RELA are upregulated in gastric cancer cell lines and tumors, and their knockdown inhibits tumor growth in vitro and in vivo. Furthermore, miR-508-3p is identified as a tumor suppressor that directly targets and downregulates NF-kB1 in gastric cancer. Re-expression of NF-kB1 partly reverses the tumor suppressive effects of miR-508-3p overexpression. In conclusion, downregulation of miR-508-3p contributes to canonical NF-kB activation in gastric tumorigenesis.
Induced Expression of CYP1A by Triclosan in Zebra Fish to measure toxicologic...
1) The study examines the effects of triclosan exposure on zebrafish by observing changes in expression of the CYP1A and TCF3A genes.
2) Using qPCR, the study found a 250% increase in CYP1A expression in triclosan-treated zebrafish compared to controls, indicating triclosan exposure induces CYP1A expression. However, a 9% increase in TCF3A expression in treated fish was not statistically significant.
3) EROD assays were also used to measure CYP1A activity, showing triclosan exposure induces CYP1A enzyme activity in zebrafish. The study suggests further research is
Darley Source Poster final
- Dopamine plays an important role in motor control and Parkinson's disease is characterized by degeneration of dopaminergic neurons.
- Previous models of Parkinson's disease in C. elegans observed neuronal degeneration via fluorescent microscopy at day 9 of development, which was time-consuming.
- This study developed a new assay to detect behavioral defects in C. elegans correlated with dopamine neuron degeneration at an earlier time point (day 3), allowing faster screening of factors that contribute to Parkinson's disease onset.
Breast final
This document summarizes the development of novel inhibitors of PAK1 for the treatment of breast cancer. It outlines assays to identify small molecules that promote PAK1 dimerization or stabilize its autoinhibited state. Hits from cytotoxicity screening will undergo PAK1 activity, dimerization, and phosphorylation assays. Promising leads will be characterized in vitro and tested in a mouse efficacy model. The goal is to develop PAK1 inhibitors with improved specificity and efficacy compared to existing drugs.
48x36_horizontal7_23_14
This study aimed to investigate mechanisms regulating expression of the tumor suppressor DAPK1 in chronic lymphocytic leukemia (CLL) cells. The researchers found that ZIPK, an interacting partner of the transcription factor ATF6, plays a critical role in IFN-γ-induced autophagy by promoting DAPK1 expression. Knockdown of ZIPK in cells prevented autophagy stimulation by IFN-γ. Additionally, primary CLL cells showed low levels of transcripts for proteins involved in autophagy induction like C/EBP-β, ATF6, and DAPK1, suggesting dysregulation of the pathway. Understanding how DAPK1 expression is controlled could help develop therapies
Seminario biomol
The document discusses the assembly and antitumor activity of lycium barbarum polysaccharide-platinum conjugates. Specifically, it investigates conjugating lycium barbarum polysaccharide (LBP) extracted from goji berries with 5-aminosalicylic acid (5-ASA) and platinum to form LBP-5-ASA-Pt conjugates. The cytotoxicity of the conjugates was tested on several cancer cell lines and a normal kidney cell line. Results showed the conjugates were cytotoxic to cancer cells, with LBP-5-ASA-Pt having the strongest effect. Further experiments showed the conjugates interacted with DNA and induced cell cycle arrest and apoptosis in cancer cells.
PERK PowerPoint
This document describes a study examining the role of the unfolded protein response (UPR) pathway protein PERK in Ebolavirus replication. The researchers generated VeroE6 cell lines with reduced PERK expression using lentiviral transduction of shRNA against PERK. They found that PERK knockdown cells had lower PERK and phosphorylated PERK levels both at baseline and during ER stress induction. These cells were then infected with Ebolavirus, and a plaque assay found that PERK knockdown cells produced significantly fewer viral plaques over time compared to control cells, suggesting PERK promotes Ebolavirus replication efficiency.
Seahorse Poster DOS (JJW Edits 6-15-15)
The study compared measurements of oxygen consumption rate (OCR) in pancreatic islets from pigs and non-human primates using an extracellular flux analyzer and a stirred microchamber method. The analyzer produced comparable OCR measurements to the standard method but with higher variability. Basal OCR was lower in non-human primate islets than pig islets, but primate islets showed a greater increase in OCR in response to hyperglycemia. Immature pig islets had almost no OCR response to glucose. The analyzer can provide species-specific and maturation state insights into islet function and mitochondrial health beyond what the standard method allows.
Bioinformatic data analysis – comparison from three human studies using diffe...
This document summarizes three bioinformatics studies comparing gene expression data from human samples using different Affymetrix platforms: 1) comparing periodontal ligament and gingival cells, 2) identifying genes regulated by nuclear IGFBP5 in osteoblasts, and 3) effects of LPS on osteoblasts. Key findings include identification of differentially expressed genes and biological processes between tissue types in study 1, and processes related to cell cycle, proliferation and splicing regulated by IGFBP5 in study 2. Study 3 on LPS effects found upregulation of factors stimulating osteoclasts and downregulation of processes like mitosis and cell cycle. The document discusses analysis tools and validation of array results.
Bio390 final paper
This document describes site-directed mutagenesis experiments performed on the global transcription factor cAMP receptor protein (CRP) in Escherichia coli. Specifically, lysine residue 36 of CRP was mutated to alanine using site-directed mutagenesis to investigate how protein acetylation may affect CRP function and gene regulation. The document provides background on CRP and its role in transcription, as well as an overview of protein acetylation and its effects. It then describes the methods used to mutate lysine 36 to alanine in CRP and plans to analyze the mutant protein.
Research Symposium Poster (Spring - April 2014)
This study aimed to assess the anti-inflammatory potential of 5,6-EET-EA. Researchers isolated splenocytes from mice and stimulated them with LPS to induce inflammation. They then treated the splenocytes with 5,6-EET-EA or anandamide (AEA) and used flow cytometry to detect levels of inflammatory cytokines IL-6 and TNF-α. The results showed that 5,6-EET-EA decreased IL-6 levels at certain concentrations, similar to the effects of AEA. This suggests that 5,6-EET-EA has anti-inflammatory properties by reducing the levels of pro-inflammatory cytokines. More research is needed to confirm these anti-inflammatory effects, including
44-150724PST_Peptalk_2013_AlphaLISA_immunoassays
The document describes the development and use of AlphaLISA assays to characterize monoclonal antibody biotherapeutics. AlphaLISA is a homogeneous bead-based immunoassay that provides advantages over ELISA, including shorter assay times without washing steps. New AlphaLISA assays have been developed for detecting fucosylated IgG, host cell proteins, and quantifying therapeutic antibodies. Data shows the assays offer sensitive and precise detection of analytes during biotherapeutic development and characterization.
Participation of the gabaergic system on the glutamate
This study investigated the participation of the GABAergic system in regulating glutamate release from frontal cortex synaptosomes in a rat model of experimental autoimmune encephalomyelitis (EAE), which mimics multiple sclerosis in humans. The results showed reduced GABAergic inhibition of glutamate release and impaired GABA regulation of synapsin I phosphorylation in synaptosomes from EAE rats compared to controls. Specifically, GABA had no effect on glutamate release or synapsin I phosphorylation in EAE rats, suggesting alterations in the GABAergic system may contribute to cortical pathology in EAE.
Jianying Xiao CV 2016
Jianying Xiao has over 16 years of experience in pharmaceutical research. She has expertise in in vivo and in vitro drug discovery techniques related to drug metabolism, infectious diseases, immunology, and cardio-metabolic disorders. She is proficient in various research techniques including animal handling, molecular biology, cell culture, and data analysis software. Jianying has worked at Merck & Co. for over 18 years, leading numerous projects that resulted in publications, patents, and awards. Her work has advanced drug programs from research through clinical trials.
Advantages And Disadvantages Of Reverse Sequence Syphilis...
Here are the key conclusions I can draw from the results of this experiment:
- The chemotherapeutic drug was effective at reducing cell viability in a dose-dependent manner across all three cancer cell lines tested (HEPG2, PANC-1, PC-3). Higher drug concentrations led to lower percentages of viable cells.
- PANC-1 and PC-3 cell lines appeared to be more sensitive to the drug, as viability decreased more sharply with increasing concentration compared to HEPG2. This suggests the drug may be more effective against pancreatic and prostate cancers.
- Even at the highest concentration of 40μM, the drug did not reduce HEPG2 viability below 60%. This indicates HEPG2 may be more
International Journal of Pharmaceutical Science Invention (IJPSI)
International Journal of Pharmaceutical Science Invention (IJPSI) is an international journal intended for professionals and researchers in all fields of Pahrmaceutical Science. IJPSI publishes research articles and reviews within the whole field Pharmacy and Pharmaceutical Science, new teaching methods, assessment, validation and the impact of new technologies and it will continue to provide information on the latest trends and developments in this ever-expanding subject. The publications of papers are selected through double peer reviewed to ensure originality, relevance, and readability. The articles published in our journal can be accessed online
Similar a Seminario biologia molecular universidad pontificia bolivariana (20)
Induced Expression of CYP1A by Triclosan in Zebra Fish to measure toxicologic...
Induced Expression of CYP1A by Triclosan in Zebra Fish to measure toxicologic...
Bioinformatic data analysis – comparison from three human studies using diffe...
Bioinformatic data analysis – comparison from three human studies using diffe...
Participation of the gabaergic system on the glutamate
Participation of the gabaergic system on the glutamate
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pathology MCQS introduction to pathology general pathology
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- Video recording of this lecture in English language: https://youtu.be/Pt1nA32sdHQ
- Video recording of this lecture in Arabic language: https://youtu.be/uFdc9F0rlP0
- Link to download the book free: https://nephrotube.blogspot.com/p/nephrotube-nephrology-books.html
- Link to NephroTube website: www.NephroTube.com
- Link to NephroTube social media accounts: https://nephrotube.blogspot.com/p/join-nephrotube-on-social-media.html
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There is increasing confidence that cell therapies will soon play a role in the treatment of autoimmune disorders, but the extent of this impact remains to be seen. Early readouts on autologous CAR-Ts in lupus are encouraging, but manufacturing and cost limitations are likely to restrict access to highly refractory patients. Allogeneic CAR-Ts have the potential to broaden access to earlier lines of treatment due to their inherent cost benefits, however they will need to demonstrate comparable or improved efficacy to established modalities.
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The Nervous and Chemical Regulation of Respiration
These lecture slides, by Dr Sidra Arshad, offer a simplified look into the mechanisms involved in the regulation of respiration:
Learning objectives:
1. Describe the organisation of respiratory center
2. Describe the nervous control of inspiration and respiratory rhythm
3. Describe the functions of the dorsal and respiratory groups of neurons
4. Describe the influences of the Pneumotaxic and Apneustic centers
5. Explain the role of Hering-Breur inflation reflex in regulation of inspiration
6. Explain the role of central chemoreceptors in regulation of respiration
7. Explain the role of peripheral chemoreceptors in regulation of respiration
8. Explain the regulation of respiration during exercise
9. Integrate the respiratory regulatory mechanisms
10. Describe the Cheyne-Stokes breathing
Study Resources:
1. Chapter 42, Guyton and Hall Textbook of Medical Physiology, 14th edition
2. Chapter 36, Ganong’s Review of Medical Physiology, 26th edition
3. Chapter 13, Human Physiology by Lauralee Sherwood, 9th edition
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Seminario biologia molecular universidad pontificia bolivariana
- 1. SARA VELEZ GONZALEZ III SEMESTER UPB
- 2. Warburg Effect Is a metabolic phenomenon observed in cancer cells where they predominantly rely on glycolysis for energy production, even in the presence of oxygen.
- 3. Angiotensin Is a peptide hormone that plays a key role in regulating blood pressure and fluid balance in the body. It is part of the renin- angiotensin system. The most biologically active form is angiotensin II.
- 4. Objective This study investigates whether Ang-(1-7) mitigates inflammation in LPS-induced macrophages and septic mice by regulating the Warburg effect in immune metabolism.
- 5. Methods WESTERN BLOT Levels of TNF-a an IL-6 in cell culture supernatant and mouse serum was measured using an Elisa kit (Enzyme-linked immunosorbent assay). The kit was brought to room temperature, standard and samples wells were prepared. The standards, samples and reagents were added according to the instructions. Absorbance at 450 nm was then measured, and sample concentrations were calculated following the manual’s instructions. In this study, RAW264.7 cells and kidney tissues were lysed to release proteins, which were then separated, transferred onto membranes, and blocked. Primary antibodies were applied overnight at 4°C, followed by secondary antibodies at room temperature. Chemiluminescent signals were used for detection. Target proteins included β-actin, TNF- α, IL-6, SLC25A1, HK2, PFKFB3, PKM2, NDUFB8, UQCRFS1, ACLY, and HIF-1α. ELISA
- 6. Methods QRT-PCR Tissues were processed with xylene and ethanol, followed by antigen retrieval and blocking with 5% BSA. They were then incubated overnight at 4°C with primary antibodies and with secondary antibodies at room temperature. DAPI staining was applied for nuclear visualization, and slides were sealed. Analysis was done using a fluorescence microscope, capturing microscopic images. The total RNA was extracted from cells or tissues using the SteadyPure Quick RNA extraction kit. The concentration and purity of the RNA samples were assessed. Next, the RNA was reverse- transcribed into cDNA using RT Master Mix for qPCR II. qPCR reactions were then carried out using SYBR Green qPCR Master Mix and the CFX96 RT- PCR system. IMMUNOFLUORE- SCENCE STAINIG
- 7. Results
- 8. Results
- 10. Conclusions The study underscores the significance of molecular biology by elucidating how Angiotensin-(1-7) influences the Warburg effect, a key metabolic pathway, in LPS-induced macrophages and septic mice By targeting molecular pathways involved in cellular metabolism, such as the Warburg effect, Angiotensin- (1-7) presents a promising approach to mitigating inflammation in septic conditions, emphasizing the intersection between molecular biology and clinical interventions.