The document discusses Escherichia coli (E. coli) and antimicrobial resistance. It describes E. coli, genes that can make it resistant to colistin (mcr-4, mcr-5, mcr-6, mcr-9), and the objectives of investigating antimicrobial resistance in broiler chickens. It outlines methods used like PCR, MIC, MLST, and antimicrobial susceptibility testing. Results shown include PCR gel images and a phylogenetic tree. The discussion evaluates studies on detecting colistin resistance and trends in resistance gene prevalence. It concludes that molecular biology techniques like MLST and PCR are important for understanding and tracking antimicrobial resistance by identifying genes and strains.