SlideShare una empresa de Scribd logo
1 de 22
Characterization of a toll-interacting protein (TOLLIP) gene in the black abalone ( Haliotis cracherodii ) Cullen Taplin May 16, 2008
Presentation Outline ,[object Object],[object Object],[object Object],[object Object],[object Object]
Black Abalone ,[object Object],[object Object],[object Object]
Abalone Distribution in Western U.S. ,[object Object]
Black Abalone Declines ,[object Object],[object Object],[object Object],[object Object],[object Object],1986 1988 1999
Withering Syndrome ,[object Object],[object Object],Healthy black abalone (left) and one suffering from withering syndrome (right). J. Moore
Abalones’ Immune Response ,[object Object],[object Object],[object Object]
Identifying Immune Related Genes ,[object Object],[object Object],[object Object]
Toll-like Receptor Pathway ,[object Object],[object Object],Singh, Bhanu P., R. S. Chauhan, and Kokesh K. Singhal. 2003.  Toll-like Receptors And Their Role In Innate Immunity . Current Science. vol. 85, no. 8, pgs. 1156 – 1164.
Research Goal & Objectives ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Abalone Used In Research ,[object Object],[object Object]
Research Methods ,[object Object],[object Object],[object Object],[object Object],[object Object]
Partial TOLLIP sequence and comparison to the Pacific abalone ( H. discus ) ,[object Object],Results: Sequence Information
TOLLIP Sequencing Results (cont.) ,[object Object],[object Object],[object Object],10kbp – 4kbp – 2kbp – 800bp –
Results: Discovering  Other TLR Genes ,[object Object],[object Object],[object Object],[object Object],Protein Analysis Through Evolutionary Relationships www.pantherdb.org/
Results: TOLLIP Expression in Digestive Gland Tissue Five months after initial exposure. N=8 for control groups, N=9 exposed groups. Means were not significantly different (p > 0.05).
Results: TOLLIP Expression in Gill Tissue Eight months after initial exposure. N = 6 for both treatment groups. Means were not significantly different (p > 0.05).
Results: Correlation of TOLLIP Expression in and Bacterial Loads No significant correlations between TOLLIP expression and bacterial loads (p > 0.05).
Summary of Results ,[object Object],[object Object],[object Object],[object Object],Data suggests the TLR pathway is active in gill tissue.
Future Work ,[object Object],[object Object],[object Object]
Acknowledgements ,[object Object],[object Object],[object Object],[object Object],[object Object]
Questions?

Más contenido relacionado

La actualidad más candente

Lecture on history of genetics
Lecture on history of geneticsLecture on history of genetics
Lecture on history of genetics
Aliah University
 
Pertemuan 2. history of genetics Bu Rani Wulandari
Pertemuan 2. history of genetics Bu Rani WulandariPertemuan 2. history of genetics Bu Rani Wulandari
Pertemuan 2. history of genetics Bu Rani Wulandari
Suryati Purba
 
Shigella flexneri serotype 1c derived from serotype 1a by acquisition of gtrI...
Shigella flexneri serotype 1c derived from serotype 1a by acquisition of gtrI...Shigella flexneri serotype 1c derived from serotype 1a by acquisition of gtrI...
Shigella flexneri serotype 1c derived from serotype 1a by acquisition of gtrI...
Swee Seong TANG
 

La actualidad más candente (20)

Discovery of dna as the universal genetic material
Discovery of dna as the universal genetic materialDiscovery of dna as the universal genetic material
Discovery of dna as the universal genetic material
 
General Genetics Lec 1
General Genetics Lec 1General Genetics Lec 1
General Genetics Lec 1
 
Aia review (1)
Aia review (1)Aia review (1)
Aia review (1)
 
Horizantal gene transfer in evolution of nematodes
Horizantal gene transfer in evolution of nematodesHorizantal gene transfer in evolution of nematodes
Horizantal gene transfer in evolution of nematodes
 
Louisville2
Louisville2Louisville2
Louisville2
 
Lecture on history of genetics
Lecture on history of geneticsLecture on history of genetics
Lecture on history of genetics
 
Conjugation
ConjugationConjugation
Conjugation
 
Cytogenetic techniques for gene location and transfer
Cytogenetic techniques for gene location and transferCytogenetic techniques for gene location and transfer
Cytogenetic techniques for gene location and transfer
 
Pertemuan 2. history of genetics Bu Rani Wulandari
Pertemuan 2. history of genetics Bu Rani WulandariPertemuan 2. history of genetics Bu Rani Wulandari
Pertemuan 2. history of genetics Bu Rani Wulandari
 
maintenance of genetic stocks
maintenance of genetic stocks maintenance of genetic stocks
maintenance of genetic stocks
 
~History of genetics:>
~History of genetics:>~History of genetics:>
~History of genetics:>
 
Classical and modern genetics
Classical and modern geneticsClassical and modern genetics
Classical and modern genetics
 
conjugation
conjugationconjugation
conjugation
 
Evidences to prove DNA as genetic material
Evidences to prove DNA as genetic materialEvidences to prove DNA as genetic material
Evidences to prove DNA as genetic material
 
History of Genetics Genetics Scope and applications of genetics
History of Genetics Genetics Scope and applications of geneticsHistory of Genetics Genetics Scope and applications of genetics
History of Genetics Genetics Scope and applications of genetics
 
Bacterial Conjugation
Bacterial ConjugationBacterial Conjugation
Bacterial Conjugation
 
DNA as genetic material
DNA as genetic materialDNA as genetic material
DNA as genetic material
 
Shigella flexneri serotype 1c derived from serotype 1a by acquisition of gtrI...
Shigella flexneri serotype 1c derived from serotype 1a by acquisition of gtrI...Shigella flexneri serotype 1c derived from serotype 1a by acquisition of gtrI...
Shigella flexneri serotype 1c derived from serotype 1a by acquisition of gtrI...
 
Knockout mice
Knockout miceKnockout mice
Knockout mice
 
Historical development of genetics final
Historical development of genetics finalHistorical development of genetics final
Historical development of genetics final
 

Similar a Taplin Capstone

Mismatch Amplification Mutation Assay (MAMA) PCR Reveals Altered El Tor Vibri...
Mismatch Amplification Mutation Assay (MAMA) PCR Reveals Altered El Tor Vibri...Mismatch Amplification Mutation Assay (MAMA) PCR Reveals Altered El Tor Vibri...
Mismatch Amplification Mutation Assay (MAMA) PCR Reveals Altered El Tor Vibri...
SSR Institute of International Journal of Life Sciences
 
Biochemistry Poster
Biochemistry PosterBiochemistry Poster
Biochemistry Poster
Zachary M. Weisner
 
An Understanding Of Bacterial Transformation By Plasmid Dna
An Understanding Of Bacterial Transformation By Plasmid DnaAn Understanding Of Bacterial Transformation By Plasmid Dna
An Understanding Of Bacterial Transformation By Plasmid Dna
Gina Buck
 
Lab Report Green Fluorescent Protein
Lab Report Green Fluorescent ProteinLab Report Green Fluorescent Protein
Lab Report Green Fluorescent Protein
Tina Jordan
 
SIPGA report 16.09 HelenWhitley
SIPGA report 16.09 HelenWhitleySIPGA report 16.09 HelenWhitley
SIPGA report 16.09 HelenWhitley
Helen Whitley
 
CJMI.000573.pdf
CJMI.000573.pdfCJMI.000573.pdf
CJMI.000573.pdf
CrimsonpublishersCJMI
 

Similar a Taplin Capstone (20)

Mismatch Amplification Mutation Assay (MAMA) PCR Reveals Altered El Tor Vibri...
Mismatch Amplification Mutation Assay (MAMA) PCR Reveals Altered El Tor Vibri...Mismatch Amplification Mutation Assay (MAMA) PCR Reveals Altered El Tor Vibri...
Mismatch Amplification Mutation Assay (MAMA) PCR Reveals Altered El Tor Vibri...
 
Fungal Contamination
Fungal ContaminationFungal Contamination
Fungal Contamination
 
History of drug resistance
History of drug resistanceHistory of drug resistance
History of drug resistance
 
Biochemistry Poster
Biochemistry PosterBiochemistry Poster
Biochemistry Poster
 
An Understanding Of Bacterial Transformation By Plasmid Dna
An Understanding Of Bacterial Transformation By Plasmid DnaAn Understanding Of Bacterial Transformation By Plasmid Dna
An Understanding Of Bacterial Transformation By Plasmid Dna
 
New insight in gastric cancer
New insight in gastric cancerNew insight in gastric cancer
New insight in gastric cancer
 
Graduate Symposium Presentation
Graduate Symposium PresentationGraduate Symposium Presentation
Graduate Symposium Presentation
 
Lab Report Green Fluorescent Protein
Lab Report Green Fluorescent ProteinLab Report Green Fluorescent Protein
Lab Report Green Fluorescent Protein
 
TILLING- Eco tilling
TILLING- Eco tillingTILLING- Eco tilling
TILLING- Eco tilling
 
Estudiante- Vanessa María Espinosa Cano
Estudiante- Vanessa María Espinosa Cano Estudiante- Vanessa María Espinosa Cano
Estudiante- Vanessa María Espinosa Cano
 
Genomic approaches to trypanosome resistance
Genomic approaches to trypanosome resistanceGenomic approaches to trypanosome resistance
Genomic approaches to trypanosome resistance
 
Jessica torres
Jessica torresJessica torres
Jessica torres
 
Bifidobacterium strain that helps reduce body fat
Bifidobacterium strain that helps reduce body fatBifidobacterium strain that helps reduce body fat
Bifidobacterium strain that helps reduce body fat
 
SIPGA report 16.09 HelenWhitley
SIPGA report 16.09 HelenWhitleySIPGA report 16.09 HelenWhitley
SIPGA report 16.09 HelenWhitley
 
Evo Paper final
Evo Paper finalEvo Paper final
Evo Paper final
 
Seminario
Seminario Seminario
Seminario
 
The expression of ITPK in normal colon and colorectal cancer cells - Paper
The expression of ITPK in normal colon and colorectal cancer cells - PaperThe expression of ITPK in normal colon and colorectal cancer cells - Paper
The expression of ITPK in normal colon and colorectal cancer cells - Paper
 
Liver enzymes in normal and sickle cell
Liver enzymes in normal and sickle cellLiver enzymes in normal and sickle cell
Liver enzymes in normal and sickle cell
 
Basic science apol1 gene and nephrocyte chaken
Basic science apol1 gene and nephrocyte chakenBasic science apol1 gene and nephrocyte chaken
Basic science apol1 gene and nephrocyte chaken
 
CJMI.000573.pdf
CJMI.000573.pdfCJMI.000573.pdf
CJMI.000573.pdf
 

Más de sr320

Genomics on the Half Shell: Making Science more Open
Genomics on the Half Shell: Making Science more OpenGenomics on the Half Shell: Making Science more Open
Genomics on the Half Shell: Making Science more Open
sr320
 
FISH441: Oysters, acidification and methylation
FISH441: Oysters, acidification and methylationFISH441: Oysters, acidification and methylation
FISH441: Oysters, acidification and methylation
sr320
 
FISH441 Group Project (Oysters)
FISH441 Group Project (Oysters)FISH441 Group Project (Oysters)
FISH441 Group Project (Oysters)
sr320
 
Timmins-Schiffman P2010
Timmins-Schiffman P2010Timmins-Schiffman P2010
Timmins-Schiffman P2010
sr320
 
Gavery PCSGA 2010
Gavery PCSGA 2010Gavery PCSGA 2010
Gavery PCSGA 2010
sr320
 

Más de sr320 (20)

Identifying Local Olympia Oyster Stocks Useful for Restoration
Identifying Local Olympia Oyster Stocks Useful for RestorationIdentifying Local Olympia Oyster Stocks Useful for Restoration
Identifying Local Olympia Oyster Stocks Useful for Restoration
 
Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?
Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?
Does DNA methylation facilitate phenotypic plasticity in marine invertebrates?
 
Science Communication and Impact: A Researcher's Perspective
Science Communication and Impact: A Researcher's PerspectiveScience Communication and Impact: A Researcher's Perspective
Science Communication and Impact: A Researcher's Perspective
 
Genomic approaches to assessing ecosystem health
Genomic approaches to assessing ecosystem healthGenomic approaches to assessing ecosystem health
Genomic approaches to assessing ecosystem health
 
Collaborative Genomic Data Analyses in the Cloud
Collaborative Genomic Data Analyses in the CloudCollaborative Genomic Data Analyses in the Cloud
Collaborative Genomic Data Analyses in the Cloud
 
Genomics on the Half Shell: Making Science more Open
Genomics on the Half Shell: Making Science more OpenGenomics on the Half Shell: Making Science more Open
Genomics on the Half Shell: Making Science more Open
 
Epigenetic and Environmental Influences on the Shellfish Immune Response
Epigenetic and Environmental Influences on the Shellfish Immune ResponseEpigenetic and Environmental Influences on the Shellfish Immune Response
Epigenetic and Environmental Influences on the Shellfish Immune Response
 
NSA2012 Short reads and Oyster Genome Resources
NSA2012 Short reads and Oyster Genome ResourcesNSA2012 Short reads and Oyster Genome Resources
NSA2012 Short reads and Oyster Genome Resources
 
Short read sequencing and shellfish
Short read sequencing and shellfishShort read sequencing and shellfish
Short read sequencing and shellfish
 
FISH441: Oysters, acidification and methylation
FISH441: Oysters, acidification and methylationFISH441: Oysters, acidification and methylation
FISH441: Oysters, acidification and methylation
 
FISH441: Oyster acidification: gene and protein expression
FISH441: Oyster acidification: gene and protein expression FISH441: Oyster acidification: gene and protein expression
FISH441: Oyster acidification: gene and protein expression
 
FISH441: Oyster Hypoxia and acclimation
FISH441: Oyster Hypoxia and acclimationFISH441: Oyster Hypoxia and acclimation
FISH441: Oyster Hypoxia and acclimation
 
Timmins Schiffman PCSGA 2011
Timmins Schiffman PCSGA 2011Timmins Schiffman PCSGA 2011
Timmins Schiffman PCSGA 2011
 
Elene Dorfmeier pcsga11
Elene Dorfmeier pcsga11 Elene Dorfmeier pcsga11
Elene Dorfmeier pcsga11
 
FISH510 Lec 1
FISH510 Lec 1FISH510 Lec 1
FISH510 Lec 1
 
FISH441 Group Project (Oysters)
FISH441 Group Project (Oysters)FISH441 Group Project (Oysters)
FISH441 Group Project (Oysters)
 
Timmins-Schiffman P2010
Timmins-Schiffman P2010Timmins-Schiffman P2010
Timmins-Schiffman P2010
 
Gavery PCSGA 2010
Gavery PCSGA 2010Gavery PCSGA 2010
Gavery PCSGA 2010
 
Salmon Senescence
Salmon SenescenceSalmon Senescence
Salmon Senescence
 
Roberts GRC
Roberts GRCRoberts GRC
Roberts GRC
 

Último

+971581248768>> SAFE AND ORIGINAL ABORTION PILLS FOR SALE IN DUBAI AND ABUDHA...
+971581248768>> SAFE AND ORIGINAL ABORTION PILLS FOR SALE IN DUBAI AND ABUDHA...+971581248768>> SAFE AND ORIGINAL ABORTION PILLS FOR SALE IN DUBAI AND ABUDHA...
+971581248768>> SAFE AND ORIGINAL ABORTION PILLS FOR SALE IN DUBAI AND ABUDHA...
?#DUbAI#??##{{(☎️+971_581248768%)**%*]'#abortion pills for sale in dubai@
 

Último (20)

ProductAnonymous-April2024-WinProductDiscovery-MelissaKlemke
ProductAnonymous-April2024-WinProductDiscovery-MelissaKlemkeProductAnonymous-April2024-WinProductDiscovery-MelissaKlemke
ProductAnonymous-April2024-WinProductDiscovery-MelissaKlemke
 
A Year of the Servo Reboot: Where Are We Now?
A Year of the Servo Reboot: Where Are We Now?A Year of the Servo Reboot: Where Are We Now?
A Year of the Servo Reboot: Where Are We Now?
 
Understanding Discord NSFW Servers A Guide for Responsible Users.pdf
Understanding Discord NSFW Servers A Guide for Responsible Users.pdfUnderstanding Discord NSFW Servers A Guide for Responsible Users.pdf
Understanding Discord NSFW Servers A Guide for Responsible Users.pdf
 
Automating Google Workspace (GWS) & more with Apps Script
Automating Google Workspace (GWS) & more with Apps ScriptAutomating Google Workspace (GWS) & more with Apps Script
Automating Google Workspace (GWS) & more with Apps Script
 
Connector Corner: Accelerate revenue generation using UiPath API-centric busi...
Connector Corner: Accelerate revenue generation using UiPath API-centric busi...Connector Corner: Accelerate revenue generation using UiPath API-centric busi...
Connector Corner: Accelerate revenue generation using UiPath API-centric busi...
 
Top 10 Most Downloaded Games on Play Store in 2024
Top 10 Most Downloaded Games on Play Store in 2024Top 10 Most Downloaded Games on Play Store in 2024
Top 10 Most Downloaded Games on Play Store in 2024
 
The 7 Things I Know About Cyber Security After 25 Years | April 2024
The 7 Things I Know About Cyber Security After 25 Years | April 2024The 7 Things I Know About Cyber Security After 25 Years | April 2024
The 7 Things I Know About Cyber Security After 25 Years | April 2024
 
+971581248768>> SAFE AND ORIGINAL ABORTION PILLS FOR SALE IN DUBAI AND ABUDHA...
+971581248768>> SAFE AND ORIGINAL ABORTION PILLS FOR SALE IN DUBAI AND ABUDHA...+971581248768>> SAFE AND ORIGINAL ABORTION PILLS FOR SALE IN DUBAI AND ABUDHA...
+971581248768>> SAFE AND ORIGINAL ABORTION PILLS FOR SALE IN DUBAI AND ABUDHA...
 
Apidays New York 2024 - Scaling API-first by Ian Reasor and Radu Cotescu, Adobe
Apidays New York 2024 - Scaling API-first by Ian Reasor and Radu Cotescu, AdobeApidays New York 2024 - Scaling API-first by Ian Reasor and Radu Cotescu, Adobe
Apidays New York 2024 - Scaling API-first by Ian Reasor and Radu Cotescu, Adobe
 
Repurposing LNG terminals for Hydrogen Ammonia: Feasibility and Cost Saving
Repurposing LNG terminals for Hydrogen Ammonia: Feasibility and Cost SavingRepurposing LNG terminals for Hydrogen Ammonia: Feasibility and Cost Saving
Repurposing LNG terminals for Hydrogen Ammonia: Feasibility and Cost Saving
 
Artificial Intelligence Chap.5 : Uncertainty
Artificial Intelligence Chap.5 : UncertaintyArtificial Intelligence Chap.5 : Uncertainty
Artificial Intelligence Chap.5 : Uncertainty
 
A Domino Admins Adventures (Engage 2024)
A Domino Admins Adventures (Engage 2024)A Domino Admins Adventures (Engage 2024)
A Domino Admins Adventures (Engage 2024)
 
Apidays Singapore 2024 - Building Digital Trust in a Digital Economy by Veron...
Apidays Singapore 2024 - Building Digital Trust in a Digital Economy by Veron...Apidays Singapore 2024 - Building Digital Trust in a Digital Economy by Veron...
Apidays Singapore 2024 - Building Digital Trust in a Digital Economy by Veron...
 
🐬 The future of MySQL is Postgres 🐘
🐬  The future of MySQL is Postgres   🐘🐬  The future of MySQL is Postgres   🐘
🐬 The future of MySQL is Postgres 🐘
 
Boost Fertility New Invention Ups Success Rates.pdf
Boost Fertility New Invention Ups Success Rates.pdfBoost Fertility New Invention Ups Success Rates.pdf
Boost Fertility New Invention Ups Success Rates.pdf
 
Strategies for Landing an Oracle DBA Job as a Fresher
Strategies for Landing an Oracle DBA Job as a FresherStrategies for Landing an Oracle DBA Job as a Fresher
Strategies for Landing an Oracle DBA Job as a Fresher
 
Exploring the Future Potential of AI-Enabled Smartphone Processors
Exploring the Future Potential of AI-Enabled Smartphone ProcessorsExploring the Future Potential of AI-Enabled Smartphone Processors
Exploring the Future Potential of AI-Enabled Smartphone Processors
 
Manulife - Insurer Innovation Award 2024
Manulife - Insurer Innovation Award 2024Manulife - Insurer Innovation Award 2024
Manulife - Insurer Innovation Award 2024
 
MINDCTI Revenue Release Quarter One 2024
MINDCTI Revenue Release Quarter One 2024MINDCTI Revenue Release Quarter One 2024
MINDCTI Revenue Release Quarter One 2024
 
Tata AIG General Insurance Company - Insurer Innovation Award 2024
Tata AIG General Insurance Company - Insurer Innovation Award 2024Tata AIG General Insurance Company - Insurer Innovation Award 2024
Tata AIG General Insurance Company - Insurer Innovation Award 2024
 

Taplin Capstone

  • 1. Characterization of a toll-interacting protein (TOLLIP) gene in the black abalone ( Haliotis cracherodii ) Cullen Taplin May 16, 2008
  • 2.
  • 3.
  • 4.
  • 5.
  • 6.
  • 7.
  • 8.
  • 9.
  • 10.
  • 11.
  • 12.
  • 13.
  • 14.
  • 15.
  • 16. Results: TOLLIP Expression in Digestive Gland Tissue Five months after initial exposure. N=8 for control groups, N=9 exposed groups. Means were not significantly different (p > 0.05).
  • 17. Results: TOLLIP Expression in Gill Tissue Eight months after initial exposure. N = 6 for both treatment groups. Means were not significantly different (p > 0.05).
  • 18. Results: Correlation of TOLLIP Expression in and Bacterial Loads No significant correlations between TOLLIP expression and bacterial loads (p > 0.05).
  • 19.
  • 20.
  • 21.