Acetyl-Histone H2A (Lys5) Antibody detects endogenous levels of histone H2A only when acetylated at lysine 5. To purchase this antibody use the following link: http://www.stjohnslabs.com/histone-h2a-acetyl-lys5-antibody?filter_name=STJ97171

1. HOW DID OUR
ANTIBODY PERFORM?
CUSTOMER REVIEW: Histone H2A (Acetyl Lys5) Polyclonal Antibody (STJ97171)

2. WHAT WAS USED?
Primary antibody: STJ97171. Histone H2A
(Acetyl Lys5) Polyclonal Antibody.
Provider: St John's Laboratory
Dilution ratio: 1:250
Application: Immunofluorescence
Materials for Validation: Drosophila 3rd instar
imaginal discs

3. WHAT WAS THE PROTOCOL?
Fixation: 4% paraformaldehyde in PBS + 0.1% Triton-X for 5
minutes.
Blocking: 5% FBS in PBS + 0.4% Triton-X for 45 minutes.
Primary antibody probing: primary antibody was added to
the solution of blocking buffer, 4°C overnight.
Wash: PBS + 0.4% Triton-X wash for 3 times.
Secondary antibody probing: secondary antibody (Alexa-
conjugated goat anti-rabbit 568) was added to the blocking
buffer with 1:500 dilution ratio, for 2 hrs.
Wash: 1X PBS + 0.4% Triton-X wash for 3 times.
Mounting: DAPI-staining conducted in PBS for 10 minutes,
followed by mounting with Prolong Gold Antifade.
Visualization: Deltavision fluorescence microscope.

4. WHAT WAS THE PROTOCOL?
Fixation: 4% paraformaldehyde in PBS + 0.1% Triton-X for 5 minutes.
Blocking: 5% FBS in PBS + 0.4% Triton-X for 30 minutes.
No. Antigen Primary antibody
Primary
antibody
dilution ratio
Secondary
antibody dilution
ratio
Co-staining
Antibody
Mounting Medium
1
Diploid imaginal
disc cells
STJ97171. Histone H2A
(Acetyl Lys5) Polyclonal
Antibody
1:250 1:500 DAPI Prolong Gold

5. WHAT DID THE CUSTOMER THINK?
Testing results were provided by Serafin Colmenares from
Lawrence Berkeley National Laboratory. They stated that
“H2AK5Ac signal overlaps with complete nucleus, except
for DAPI bright heterochromatic region. This is in line
with the expected signal for this specific modification.”

6. “WE INVITE YOU TO TEST OUR PRODUCTS”
Our aim is to not only connect our customers
with the best product, but to also give them
the opportunity to test the product’s
performance risk free.

7. Contact Us: info@stjohnslabs.com
1. Choose 5 Trial Size Samples from our free sample list
2. Complete the free sample application: http://www.stjohnslabs.com/free-sample
3. Receive your antibody
4. Submit your review
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