1. International course on
Developmental Biology
October 2011
Virginie Mournetas – 11/2011

2. Course presentation
 In Paris:
• Pierre and Marie Curie University
• Curie institute
 For Master and PhD students
 Theoretical and experimental training

3. Course presentation
 Topics:
• Embryonic stem cells
• Cell specification and differentiation
• Pattern formation
• Morphogenesis and organogenesis
• Intracellular signalling
• Developmental regulation of gene expression
 Schedules:
• 3 weeks of practicals
• 2 weeks of lectures

4. Schedule
 Practicals (3 weeks):
• Mouse
• Early mouse Development
• Somitogenesis and organogenesis
• Mouse embryo culture
• Chick
• Drosophila
• Xenopus
• C. elegans
• Zebrafish
 Lectures (2 weeks)

5. Practicals
Early mouse
Development
K. Wassmann
JP. Chambon

6. Early mouse
Practicals
Development
 Mouse dissection
 Oocyte recovery
o Prophase
o Metaphase II
 Whole-mount immunofluorescence

7. Early mouse Results
Development Immunofluorescence
Metaphase I Metaphase II
CREST
DNA (DAPI)

8. Early mouse Results
Development Chromosomes spread
Metaphase I Metaphase II
CREST
DNA (propidium iodie)

9. Practicals
Mouse embryo culture:
somitogenesis and
organogenesis
C. Fournier R. Grifonne M. Paces-Fessy
S. Escot
P. Trainor S. Cereghini S. Escot

10. Mouse embryo
Practicals
culture
 Mouse embryo dissection
o At different stages
 Organ culture
 Urogenital tract development
(Lac-Z knock-in line)

11. Practicals
Chick
C. Drevon C. Thibault
T. Jaffredo S. Escot

12. Chick 3 days old chick embryo

13. Chick Practicals
 Embryo culture Chapman, 2001

14. Chick Practicals
 Embryo culture
 Neural tube culture
Duband et al.

15. Chick Practicals
 Embryo culture
 Neural tube culture
 Quail-chick graft: Grafting the neural tube

16. Chick Practicals
 Embryo culture
 Neural tube culture
 Quail-chick graft: Grafting the neural tube
 Electroporation

17. Practicals
Drosophila
A. Audibert A. Ramat
M. Gho

18. Drosophila Practicals
 Imaginal disc dissection
(Third instar larva stage)

19. Drosophila Practicals
 Imaginal disc dissection
(Third instar larva stage)
 Adult wing dissection

20. Practicals
Xenopus
JF Riou C. Carron
M. Umbhauer O. Bronchain

21. Xenopus Practicals
 Embryo culture
 Embryo microdissection
 Embryo injection
 Computer analysis
(Morpholinos)

22. Practicals
C. Elegans
S. Louvet
V. Galy

23. C. Elegans Practicals
 Analyse of P granules
(localization and segregation)

24. Practicals
Zebrafish
E. Havis F. Giudiceli
E. Hirsinger

25. Zebrafish Practicals
 Embryo microdissection
 Embryo injection

26. LECTURES

27. Speakers Lab Title
Enrique AMAYA University of Manchester, GB The secret of scarless wound healing and appendage regeneration
Lectures
Guillaume BALAVOINE IJM, FR The role of signalling pathways in segment formation: was Urbilateria segmented?
Allison BARDIN Curie Institute, Paris, FR Maintenance of adult tissues by tissues-specific stem cells
Yohanns BELLAICHES Curie Institute, Paris, FR Epithelial morphogenesis
Deborah BOURC’HIS Curie Institute, Paris, FR Epigenetic decisions and reproduction in mammals
Julius BRENNECKE IMBA, Vienna, AU The piRNA pathway in the drosophila germline: guradian of the genome
Nicolas DAVID ENS, Paris, FR Gastrulation movements in fish, focus on prechordal plate migration
Filippo DEL BENE Curie Institute, Paris, FR Zebrafish visual system development and function: imaging sutdies
Ian DRUMMOND Harvard University, Boston, USA Insights into epithelial organ development and morphogenesis from the zebrafish
Charles FFRENCH-CONSTANT University of Edinburgh, SC The microenvironment of the embryonic neural stem cell: lessons from adult niches
Anne GRAPIN EPFL, Lausanne, SW Pancreas development, stem cells and cancer
Edith HEARD Institut Curie, FR Developmental dynamics and evolutionary diversity of X-chromosome inactivation in mammals
Philippe HERBOMEL Pasteur Institute, Paris, FR The zebrafish reveals the Odyssey of hematopoietic precursors in developing vertebrate embryos
Thomas HOLSTEIN University of Heidelberg, GE Stem cells in Cnidian regeneration
Thierry JAFFREDO UMR7622, Paris, FR Hematopoietic development in vertebrates: rules and paradigms
Laurent KODJABACHIAN IGBMC, Marseille, FR Motile ciliogenesis in vertebrates
Michel LABOUESSE IGBMC, Strasbourg, FR Signalling through mechanical input: a coordinated process
Thomas LECUIT IBDM, Marseille, FR Genetic and mechanical regulation of morphogenesis
Patrick LEMAIRE CRBM, Montpellier Exploring the diversity of Tunicate genomes and developmental programmes
Roberto MAYOR University College, London, GB Collective cell migration. The case of the neural crest
Olivier POURQUIÉ IGBMC, Strasbourg, FR Developmental patterning of the vertebrate embryonic axis
Frédéric RELAIX Myology Institute, Paris, FR Skeletal muscle stem and progenitor cells during development
François SCHWEISGUTH Pasteur Institute, Paris, FR Asymmetric cell division in drosophila
Daniel ST JOHNSTON Cambridge university, GB Polarising the anterior-posterior and dorsal-ventral axes in dorsophila
Gastrulation through a primitive streak: cellular mechanisms and signals
Claudio STERN University College, London, GB Building a brain: a molecular dissection of neural induction
Cliff TABIN Harvard University, Boston, USA New insights in vertebrate limb development
Jan TRAAS ENS, Lyon, FR From genes to shape at the shoot meristem
Paul TRAINOR Stowers Institute, Kansas City, USA Neural crest cells: evolution development and disease