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Biotech 2011-06-electrophoresis-blots

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Nikolay Vyahhi
Nikolay Vyahhi
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Electrophoresis and Blots
Principles recap ,[object Object],[object Object],[object Object]
Agarose versus acrylamide ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Restriction enzymes I ,[object Object],[object Object],[object Object],[object Object],[object Object]
Enzymes ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Restriction enzymes III ,[object Object],[object Object],Size of recognition sequence Average size of fragment 4 256 5 1024 6 4096 8 65536
Agarose gel electrophoresis ,[object Object],[object Object],[object Object],[object Object]
Size determination ,[object Object],[object Object],[object Object],[object Object],[object Object]
Digesting human chromosomal DNA ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Southern Blot ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Probe ,[object Object],[object Object],[object Object],[object Object],[object Object]
Hybridization ,[object Object],[object Object],[object Object],[object Object],[object Object]
X-ray film exposure ,[object Object],[object Object],[object Object],[object Object],[object Object]
Overall ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Restriction fragment length polymorphism (RFLP) ,[object Object],[object Object],[object Object],[object Object]
Example I RFLP point mutation ,[object Object],[object Object],[object Object],[object Object],[object Object]
DNA from normal individual digested with Eco R1 and electrophoresed through agarose DNA from mutant individual digested with Eco R1 and electrophoresed through agarose
Example II RFLP deletion mutation How could these two patterns arise by deletion?
Example III Amplification
Other blots Northern ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Protein electrophoresis ,[object Object],[object Object],[object Object],[object Object],[object Object]
SDS-polyacrylamide gel electrophoresis ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Other blots II Western ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

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Biotech 2011-06-electrophoresis-blots