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Optimization of Immunostaining techniques in  Stenostomum virginianum  (Catenulida) Justin Waterfield Winthrop University Mentor:  Julian Smith III
Phylogeny of Platyhelminthes ,[object Object],[object Object],[object Object],[object Object],Smith  et al  (1986)
Neoblasts ,[object Object],[object Object],[object Object],Smith  et al  (1986)
Cell Cycle ,[object Object],[object Object],[object Object],From Ross & Pawlina, 2006
How does immunofluorescence work? ,[object Object],[object Object],[object Object],[object Object],1° Ab binding: (mouse anti-PCNA / rabbit anti-phosphoH3) 2° Ab binding: (Dye-labeled donkey anti-rabbit / donkey anti-mouse) Wash away excess Ab
Dye list ,[object Object],[object Object],[object Object],[object Object],[object Object]
Methods and Hypotheses ,[object Object],Formaldehyde Osmium tetroxide
Methods and Hypotheses ,[object Object]
Methods and Hypotheses ,[object Object]
Osmium Fixed ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Image I Image II
Acetone/formaldehyde Fixed ,[object Object],[object Object],[object Object],Image I Image II
Methacarn ,[object Object],[object Object],[object Object],[object Object],Image I
1-200 Dilution ,[object Object],[object Object],[object Object],[object Object],[object Object],Image I Image II
1-500 Dilution ,[object Object],[object Object],[object Object],[object Object],[object Object],Image I Image I Image II Image I
Overall Distribution ,[object Object],[object Object],[object Object],[object Object],Image I
Conclusions ,[object Object],[object Object],[object Object]
What next? ,[object Object],[object Object]
References ,[object Object],[object Object],[object Object],[object Object]
Acknowledgements ,[object Object],[object Object],[object Object],[object Object]
Questions?

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Optimization of Immunostaining techniques in Stenostomum virginianum

  • 1. Optimization of Immunostaining techniques in Stenostomum virginianum (Catenulida) Justin Waterfield Winthrop University Mentor: Julian Smith III
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