Optimization of Immunostaining techniques in Stenostomum virginianum

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Justin Waterfield

Undergraduate research at Winthrop University

Tecnología

Optimization of Immunostaining techniques in Stenostomum virginianum (Catenulida) Justin Waterfield Winthrop University Mentor: Julian Smith III

Phylogeny of Platyhelminthes ,[object Object],[object Object],[object Object],[object Object],Smith et al (1986)

Neoblasts ,[object Object],[object Object],[object Object],Smith et al (1986)

Cell Cycle ,[object Object],[object Object],[object Object],From Ross & Pawlina, 2006

How does immunofluorescence work? ,[object Object],[object Object],[object Object],[object Object],1° Ab binding: (mouse anti-PCNA / rabbit anti-phosphoH3) 2° Ab binding: (Dye-labeled donkey anti-rabbit / donkey anti-mouse) Wash away excess Ab

Dye list ,[object Object],[object Object],[object Object],[object Object],[object Object]

Methods and Hypotheses ,[object Object],Formaldehyde Osmium tetroxide

Osmium Fixed ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Image I Image II

Acetone/formaldehyde Fixed ,[object Object],[object Object],[object Object],Image I Image II

Methacarn ,[object Object],[object Object],[object Object],[object Object],Image I

1-200 Dilution ,[object Object],[object Object],[object Object],[object Object],[object Object],Image I Image II

1-500 Dilution ,[object Object],[object Object],[object Object],[object Object],[object Object],Image I Image I Image II Image I

Overall Distribution ,[object Object],[object Object],[object Object],[object Object],Image I

Conclusions ,[object Object],[object Object],[object Object]

What next? ,[object Object],[object Object]

References ,[object Object],[object Object],[object Object],[object Object]

Acknowledgements ,[object Object],[object Object],[object Object],[object Object]

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