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Effectiveness of Eucalyptus camaldulensis and
Cymbopogon citratus essential oils in protecting
rice stocks from Sitophilus oryzae and Sitotroga
cerealella.
A. Togola, P.A. Seck, F.E. Nwilene, I.A. Glitho et D. Chougourou
OUTLINE
Introduction
Material & methods
Results
Conclusion
INTRODUCTION


Rice production

• Rice is a staple food for
millions of people in Africa

•

Since 2008 its cropping area
and production have increased
in West Africa (FAOSTAT, 2011)

•

Unlike the pass , rice is more
and more stored for seeds,
consumption and trade
INTRODUCTION
 Storage issues

• Rodents, insects, fungus, abiotic
factors, etc.

• Quantitative losses due to
insects reach 5% and 30% in 4
and 6 month of storage.

• Qualitative losses : physical,
culinary and nutritional

• Economic losses: leading to the
decrease in income of the actors
of rice sector
INTRODUCTION
The objective of this study is:
To develop alternative technologies (to
chemical methods) for an effective integrated
protection of stored rice against insects pests.
MATERIAL & METHODS
Animal material:
Sitophilus oryzae
(Coleoptera: Curculionidae)

Larve

Adulte

Sitotroga cerealella
(Lepidoptera: Gelechiidae)

Larve

Adulte
Plant material:
Eucalyptus camaldulensis
(Myrtaceae)

Cymbopogon citratus
(Poaceae)

Essential oil

7
• Impregnation of gunny bags
(sacs en jute)

Alcohol
Eucalyptus camaldulensis
3%, 4%, 5%, untreated check,
Check with alcohol alone

Cymbopogon citratus
3%, 4%, 5% , untreated
check, Check with alcohol
alone

Replication: 4
• Infestation method in impregnated gunny bags
20

20

Paddy rice: 100g
8
• Treatment of cotton bags (sacs en cretonne)
Cymbopogon + cotton bag ( dose : 0.25ml/bag)
Eucalyptus + cotton bag ( dose : 0.25ml/bag)
Eucalyptus + Cymbopogon + cotton bag
Cymbopogon + cotton bag + plastic film
Eucalyptus + cotton bag + plastic film
Eucalyptus + Cymbopogon + cotton bag + plastic film
Check with cotton bag + plastic film
Check with cotton bag alone
Rep 4

• Infestation method in cotton bags
20

20

Paddy rice: 100g
9
Data collected at 90 days after treatment:

• Population of emerged adults
• Percentage of weight loss (%)

10
RESULTS
Table 1: Effect of essential oils on Sitophilus oryzae and Sitotroga
cerealella population emerged in stored paddy rice inside gunny bags (Sac
s en jute)

Treatments

Emerged
population of
S. oryzae

Emerged population
of
S. cerealella

Untreated check

51.0 ± 6.2a

345.7 ± 24,9a

Check with alcohol alone
C. citratus 3 %

48.1 ± 3.4a
51.5 ± 8.4a

329.6 ± 21,5a
113.2 ± 9,0b

C. citratus 4 %
C. citratus 5 %
E. camaldulensis 3 %
E. camaldulensis 4 %
E. camaldulensis 5 %

37.2 ± 3.4a
21.2 ± 2.6b
20.2 ± 2.0b
15.2 ± 2.6b
9.5 ± 3.1b

98.5 ± 8,8b
88.7 ± 4,0b
263.7 ± 20,4a
280.0 ± 19,4a
266.5 ± 32,4a
11
Weight losses of paddy rice (%)

10

a

a

8
6
4

b
c
d

2
0

Figure 1: weight losses recorded 90 days after storage in ganny bags
impregnated withCymbopogon citratus
Table 2: Impact of cotton bags treatment on paddy rice weight losses
(Sacs en cretonne).

Traitements
Check with cotton bag alone

Pertes pondérales
Enregistrées (%)
8,69b

Check with cotton bag + plastic film

4,78ab

Cymbopogon + cotton bag
Eucalyptus + cotton bag
Eucalyptus + Cymbopogon + cotton bag
Cymbopogon + cotton bag + plastic film
Eucalyptus + cotton bag+ plastic film
Eucalyptus + Cymbopogon + cotton bag + PF

3,53ab
3,88ab
4,79ab
3,14ab
3,76ab
1,83a
CONCLUSION
The use of the essential oils of C. citratus and E.

camalsulensis appeared to be a promising method for
protecting stored rice against S. cerealella and S. oryzae.
However, the method needs some improvement in order to
make the technology cheaper for a large access by end
users.

14
Thank you for your kind attention

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The3_Effectiveness of Eucalyptus camaldulensis and Cymbopogon citratus essential oils in protecting rice stocks from Sitophilus oryzae and Sitotroga cerealella.

  • 1. Effectiveness of Eucalyptus camaldulensis and Cymbopogon citratus essential oils in protecting rice stocks from Sitophilus oryzae and Sitotroga cerealella. A. Togola, P.A. Seck, F.E. Nwilene, I.A. Glitho et D. Chougourou
  • 3. INTRODUCTION  Rice production • Rice is a staple food for millions of people in Africa • Since 2008 its cropping area and production have increased in West Africa (FAOSTAT, 2011) • Unlike the pass , rice is more and more stored for seeds, consumption and trade
  • 4. INTRODUCTION  Storage issues • Rodents, insects, fungus, abiotic factors, etc. • Quantitative losses due to insects reach 5% and 30% in 4 and 6 month of storage. • Qualitative losses : physical, culinary and nutritional • Economic losses: leading to the decrease in income of the actors of rice sector
  • 5. INTRODUCTION The objective of this study is: To develop alternative technologies (to chemical methods) for an effective integrated protection of stored rice against insects pests.
  • 6. MATERIAL & METHODS Animal material: Sitophilus oryzae (Coleoptera: Curculionidae) Larve Adulte Sitotroga cerealella (Lepidoptera: Gelechiidae) Larve Adulte
  • 8. • Impregnation of gunny bags (sacs en jute) Alcohol Eucalyptus camaldulensis 3%, 4%, 5%, untreated check, Check with alcohol alone Cymbopogon citratus 3%, 4%, 5% , untreated check, Check with alcohol alone Replication: 4 • Infestation method in impregnated gunny bags 20 20 Paddy rice: 100g 8
  • 9. • Treatment of cotton bags (sacs en cretonne) Cymbopogon + cotton bag ( dose : 0.25ml/bag) Eucalyptus + cotton bag ( dose : 0.25ml/bag) Eucalyptus + Cymbopogon + cotton bag Cymbopogon + cotton bag + plastic film Eucalyptus + cotton bag + plastic film Eucalyptus + Cymbopogon + cotton bag + plastic film Check with cotton bag + plastic film Check with cotton bag alone Rep 4 • Infestation method in cotton bags 20 20 Paddy rice: 100g 9
  • 10. Data collected at 90 days after treatment: • Population of emerged adults • Percentage of weight loss (%) 10
  • 11. RESULTS Table 1: Effect of essential oils on Sitophilus oryzae and Sitotroga cerealella population emerged in stored paddy rice inside gunny bags (Sac s en jute) Treatments Emerged population of S. oryzae Emerged population of S. cerealella Untreated check 51.0 ± 6.2a 345.7 ± 24,9a Check with alcohol alone C. citratus 3 % 48.1 ± 3.4a 51.5 ± 8.4a 329.6 ± 21,5a 113.2 ± 9,0b C. citratus 4 % C. citratus 5 % E. camaldulensis 3 % E. camaldulensis 4 % E. camaldulensis 5 % 37.2 ± 3.4a 21.2 ± 2.6b 20.2 ± 2.0b 15.2 ± 2.6b 9.5 ± 3.1b 98.5 ± 8,8b 88.7 ± 4,0b 263.7 ± 20,4a 280.0 ± 19,4a 266.5 ± 32,4a 11
  • 12. Weight losses of paddy rice (%) 10 a a 8 6 4 b c d 2 0 Figure 1: weight losses recorded 90 days after storage in ganny bags impregnated withCymbopogon citratus
  • 13. Table 2: Impact of cotton bags treatment on paddy rice weight losses (Sacs en cretonne). Traitements Check with cotton bag alone Pertes pondérales Enregistrées (%) 8,69b Check with cotton bag + plastic film 4,78ab Cymbopogon + cotton bag Eucalyptus + cotton bag Eucalyptus + Cymbopogon + cotton bag Cymbopogon + cotton bag + plastic film Eucalyptus + cotton bag+ plastic film Eucalyptus + Cymbopogon + cotton bag + PF 3,53ab 3,88ab 4,79ab 3,14ab 3,76ab 1,83a
  • 14. CONCLUSION The use of the essential oils of C. citratus and E. camalsulensis appeared to be a promising method for protecting stored rice against S. cerealella and S. oryzae. However, the method needs some improvement in order to make the technology cheaper for a large access by end users. 14
  • 15. Thank you for your kind attention