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Variation and Selection for Cooking
 time in Drought tolerant Canning Beans
           (Phaseolus vulgaris)
                          Warsame, A. O., P. M. Kimani, and J.W. Mwangi
       Department of Plant Science and Crop Protection, College of Agriculture and Veterinary
                                  Sciences, University of Nairobi


                           First Bio-Innovate Regional Scientific Conference
                            United Nations Conference Centre (UNCC-ECA)
                             Addis Ababa, Ethiopia, 25-27 February 2013



University of Nairobi           ISO 9001:2008   1   Certified            http://www.uonbi.ac.ke
Contents

          Introduction
          Materials and Methods
          Results &Discussion
          Conclusions
          Acknowledgements




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Introduction
     Common beans is one of most import crops in eastern Africa (Pachico,
     1993; Kimani, 2001)
     Cooking time of dry bean is an important trait related to its utilization
     (Iyer et al., 1980; Miles and Sonde, 2004; Elia, 2003).
     Bean cooking time can vary from ½ hour to 8 hours (Miles and Sonde,
     2004).
     In Kenya, traditional bean varieties need 2-3 hours to cook (Kimani,
     2001).
     Cooking time is import for both canning industry and household
     consumption.
     For processors, long cooking is associated with higher energy
     requirements and lower processor yields (Van Loggerenberg, 2004).
     Urban consumers spend more money on expensive fuels.
     Rural households use large quantities of firewood and spend time in
     searching it.
University of Nairobi       ISO 9001:2008   3   Certified      http://www.uonbi.ac.ke
Continued……
     Thus, faster-cooking bean cultivars would:
    save energy and deliver higher processor yield for canning industry
    save money spent on kerosene, charcoal, electricity, and natural gas
     for or rural and urban consumers.
    Save forests.
     However, at present there is a little information on fast cooking bean
     varieties in the region.
     Processing industry in Kenya depend on single variety (Mex-142)
     which is highly susceptible to rust and anthracnose, and is low
     yielding.
     Therefore, there is need for new faster cooking bean varieties which
     combine other important traits like disease resistance, drought
     tolerance and other agronomic traits, and also meet consumer
     preferences for seed colors.

University of Nairobi      ISO 9001:2008   4   Certified     http://www.uonbi.ac.ke
Objectives

            The objective of this study was to evaluate the
            cooking time of advanced canning bean lines
            previously selected for drought tolerance, resistance
            to diseases and other agronomic traits




University of Nairobi     ISO 9001:2008   5   Certified   http://www.uonbi.ac.ke
Material and Methods
     Bean seeds used in this study were obtained
     from Grain Legume Research Program of the
     University of Nairobi, which fall in 7 market          Market class          Number of
     classes.                                                                       lines
     Mex142 was used as check                               Red mottled              22
     155 Bean lines of diverse market classes were          Red kidneys              23
     grown and selected at Kabete Field station,            Speckled Sugars          22
     University of Nairobi during the 2012 long-rain
     season season.                                         Pintos and Cariocas      22
     Seeds were soaked for 16 hours and cooked
     using Matson Bean cooker (Matson, 1946) and            Small reds               22
     trial was repeated three times.                        Navys                    24
     Analyses of variance (ANOVA) appropriate to a          Mixed Colors             20
     randomized complete-block design was
     performed using GenStat 13.3 (VSN
     International Ltd., UK.) at significance level         Total                    155
     (0.01).
University of Nairobi       ISO 9001:2008   6   Certified           http://www.uonbi.ac.ke
Continued….
                                         Small red
                                                                          Selection
                  Red
                                                                          in the
                  mottled
                                                                          field
                                          Pinto


                  Red                                                     Soaking
                  kidney                  Carioca

                                                                               Matson
                   Speckled               Yellow                               Bean
                   sugar                                                       Cooker


                                          Tan
                  Navy

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Results


       Analysis of variance showed that
       there were highly significant (P<0.01)
       genotypic differences for cooking
       time within and among market
       classes.


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Fig 1. Cooking time of advanced Red Mottled canning bean lines
                                         selected at Kabete Field Station, 2012
                  50
                            46.3
                  45               42.9          41.9                                                    42.1          41.9                             43
                                                                      40.7                                                                                          40.7          41.6
                                                               38.9                               38.3
                  40
                                                                                           36.1                 35.8                 36.3
                                          34.3                                      34.9                                                                                   34.4
                  35                                    33.1                                                                                       33        32.2
                                                                             31.8                                             31.4          30.6
        Minutes




                  30

                  25

                  20

                  15

                  10

                   5
                       0
                  0




                                                                                               Genotype
Mean              37.48
LSD (0.05)        2.801
SE                0.567
CV (%)             1.5
 University of Nairobi                                         ISO 9001:2008               9         Certified                                http://www.uonbi.ac.ke
Fig 2. Cooking time of advanced Red Kidney canning bean lines
                                     selected at Kabete Field Station, 2012

                55
                                     48.8
                50                                                           46.3
                45                                                                                                                                         43                 42.4
                              41.6                                                                                                    40.9          41.4
                                            39.1                      39.5          38.7 38.7
                40                                 36.6 37.8                                                                                 35.6
                                                                                                            35.2
                35                                             32.6                             32.1 32.1
                       31.3                                                                                               31.1 31.8
                                                                                                                                                                29.6
                                                                                                                                                                       27.8
      Minutes




                30                                                                                                 27.1
                25
                20
                15
                10
                 5
                0




                     Mean              36.77
                                                                                                Line
                     LSD (0.05)        2.973
                     SE                0.265
                     CV (%)             3.7


University of Nairobi                                          ISO 9001:2008             10       Certified                             http://www.uonbi.ac.ke
Fig 3. Cooking time of advanced speckled sugars
                                    canning bean lines selected at Kabete Field
                60
                                                   Station, 2012
                                                                                  51.53                                                                                             50.83
                50
                                                                                                                          43.27
                                                                           41.1                           41.73
                                             39.03                                                                                             38.7                   39.7
                40           34.87
                                     37.6
                                                                                                  35.27                                                                      36.1
                      35.3                                                                                        34.73
      Minutes




                                                             33.4                                                                 33.73
                                                     32.43                                                                                                    32.17
                                                                    30.4                                                                  31                                                30.43
                                                                                          29.67                                                       30.13
                30


                20


                10


                 0




                     Mean                   36.66
                     LSD (0.05)             3.797                                                         Line
                     SE                     0.033
                     CV (%)                  2.1

University of Nairobi                                               ISO 9001:2008                   11            Certified                                   http://www.uonbi.ac.ke
Fig 4. Cooking time of advanced Navy Bean lines selected at Kabete
                    Field Station, 2012
            60.0


                                 49.8 48.9          48.9
            50.0
                                                           43.3
                   41.7                      41.3                                                                                                                               42.0
                                                                                                                                 40.5
                                                                  39.3                        39.3
            40.0                                                                                                                                                         37.9
                                                                                       35.3                                             35.9          36.3 36.4
                                                                                                            34.0                               34.6
  Minutes




                                                                         33.8
                          32.2                                                  31.8                                      32.0
                                                                                                                   30.4                                           29.6
            30.0
                                                                                                     24.4

            20.0



            10.0



             0.0




            Mean             37.21
            LSD(0.05)        3.836                                                             Line
            SE               2.337
            CV(%)             1.5

University of Nairobi                                       ISO 9001:2008                 12         Certified                                 http://www.uonbi.ac.ke
Fig 5. Cooking time of advanced Small Red canning bean lines
                                         selected at Kabete Field Station, 2012

          60.0
                                                                                                                                        52.4
          50.0
                                                                             41.8                                                42.3
                                                        40.8                                    39.6                                                                40.5
                 38.7                                                                                                                                        38.3
          40.0                                                                                                                                        36.9
                                                                                    35.4 35.4                             35.3                 35.1
Minutes




                                       33.7 32.5                                                       34.5
                         31.6                    32.0                 31.2
                                                               28.7                                           29.8 30.6
          30.0                  27.1


          20.0


          10.0


           0.0




            Mean                37.21                                                  Line
            LSD(0.05)           3.836
            SE                  2.337
            CV(%)                1.5
Conclusions
     Results of this work showed significant variation in cooking
     among bean lines across market classes.
     More than 60 lines from different market classes cooked
     faster than the industry standard Mexican-142.
     This genetic variation for cooking time, can combined with
     drought tolerance, resistance to diseases and other
     agronomic traits.
     Such lines will save time, energy for rural and urban poor in
     cities and processors.
     A broad range of Consumer preferences can be met.
     Increased utilization of beans and income for farmers.
University of Nairobi     ISO 9001:2008   14   Certified   http://www.uonbi.ac.ke
Acknowledgements


  The University of Nairobi
  Bio-Innovate-Africa program
  Beans for Climate Change Consortium
  Supervisors
  Bean Program Technical staff-UON
  Governments of Kenya, Sweden-Sida and Somalia



University of Nairobi   ISO 9001:2008   15   Certified   http://www.uonbi.ac.ke
THANK YOU




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Variation and selection for cooking time in drought tolerant canning beans (Phaseolus vulgaris)

  • 1. Variation and Selection for Cooking time in Drought tolerant Canning Beans (Phaseolus vulgaris) Warsame, A. O., P. M. Kimani, and J.W. Mwangi Department of Plant Science and Crop Protection, College of Agriculture and Veterinary Sciences, University of Nairobi First Bio-Innovate Regional Scientific Conference United Nations Conference Centre (UNCC-ECA) Addis Ababa, Ethiopia, 25-27 February 2013 University of Nairobi ISO 9001:2008 1 Certified http://www.uonbi.ac.ke
  • 2. Contents Introduction Materials and Methods Results &Discussion Conclusions Acknowledgements University of Nairobi ISO 9001:2008 2 Certified http://www.uonbi.ac.ke
  • 3. Introduction Common beans is one of most import crops in eastern Africa (Pachico, 1993; Kimani, 2001) Cooking time of dry bean is an important trait related to its utilization (Iyer et al., 1980; Miles and Sonde, 2004; Elia, 2003). Bean cooking time can vary from ½ hour to 8 hours (Miles and Sonde, 2004). In Kenya, traditional bean varieties need 2-3 hours to cook (Kimani, 2001). Cooking time is import for both canning industry and household consumption. For processors, long cooking is associated with higher energy requirements and lower processor yields (Van Loggerenberg, 2004). Urban consumers spend more money on expensive fuels. Rural households use large quantities of firewood and spend time in searching it. University of Nairobi ISO 9001:2008 3 Certified http://www.uonbi.ac.ke
  • 4. Continued…… Thus, faster-cooking bean cultivars would:  save energy and deliver higher processor yield for canning industry  save money spent on kerosene, charcoal, electricity, and natural gas for or rural and urban consumers.  Save forests. However, at present there is a little information on fast cooking bean varieties in the region. Processing industry in Kenya depend on single variety (Mex-142) which is highly susceptible to rust and anthracnose, and is low yielding. Therefore, there is need for new faster cooking bean varieties which combine other important traits like disease resistance, drought tolerance and other agronomic traits, and also meet consumer preferences for seed colors. University of Nairobi ISO 9001:2008 4 Certified http://www.uonbi.ac.ke
  • 5. Objectives The objective of this study was to evaluate the cooking time of advanced canning bean lines previously selected for drought tolerance, resistance to diseases and other agronomic traits University of Nairobi ISO 9001:2008 5 Certified http://www.uonbi.ac.ke
  • 6. Material and Methods Bean seeds used in this study were obtained from Grain Legume Research Program of the University of Nairobi, which fall in 7 market Market class Number of classes. lines Mex142 was used as check Red mottled 22 155 Bean lines of diverse market classes were Red kidneys 23 grown and selected at Kabete Field station, Speckled Sugars 22 University of Nairobi during the 2012 long-rain season season. Pintos and Cariocas 22 Seeds were soaked for 16 hours and cooked using Matson Bean cooker (Matson, 1946) and Small reds 22 trial was repeated three times. Navys 24 Analyses of variance (ANOVA) appropriate to a Mixed Colors 20 randomized complete-block design was performed using GenStat 13.3 (VSN International Ltd., UK.) at significance level Total 155 (0.01). University of Nairobi ISO 9001:2008 6 Certified http://www.uonbi.ac.ke
  • 7. Continued…. Small red Selection Red in the mottled field Pinto Red Soaking kidney Carioca Matson Speckled Yellow Bean sugar Cooker Tan Navy University of Nairobi ISO 9001:2008 7 Certified http://www.uonbi.ac.ke
  • 8. Results Analysis of variance showed that there were highly significant (P<0.01) genotypic differences for cooking time within and among market classes. University of Nairobi ISO 9001:2008 8 Certified http://www.uonbi.ac.ke
  • 9. Fig 1. Cooking time of advanced Red Mottled canning bean lines selected at Kabete Field Station, 2012 50 46.3 45 42.9 41.9 42.1 41.9 43 40.7 40.7 41.6 38.9 38.3 40 36.1 35.8 36.3 34.3 34.9 34.4 35 33.1 33 32.2 31.8 31.4 30.6 Minutes 30 25 20 15 10 5 0 0 Genotype Mean 37.48 LSD (0.05) 2.801 SE 0.567 CV (%) 1.5 University of Nairobi ISO 9001:2008 9 Certified http://www.uonbi.ac.ke
  • 10. Fig 2. Cooking time of advanced Red Kidney canning bean lines selected at Kabete Field Station, 2012 55 48.8 50 46.3 45 43 42.4 41.6 40.9 41.4 39.1 39.5 38.7 38.7 40 36.6 37.8 35.6 35.2 35 32.6 32.1 32.1 31.3 31.1 31.8 29.6 27.8 Minutes 30 27.1 25 20 15 10 5 0 Mean 36.77 Line LSD (0.05) 2.973 SE 0.265 CV (%) 3.7 University of Nairobi ISO 9001:2008 10 Certified http://www.uonbi.ac.ke
  • 11. Fig 3. Cooking time of advanced speckled sugars canning bean lines selected at Kabete Field 60 Station, 2012 51.53 50.83 50 43.27 41.1 41.73 39.03 38.7 39.7 40 34.87 37.6 35.27 36.1 35.3 34.73 Minutes 33.4 33.73 32.43 32.17 30.4 31 30.43 29.67 30.13 30 20 10 0 Mean 36.66 LSD (0.05) 3.797 Line SE 0.033 CV (%) 2.1 University of Nairobi ISO 9001:2008 11 Certified http://www.uonbi.ac.ke
  • 12. Fig 4. Cooking time of advanced Navy Bean lines selected at Kabete Field Station, 2012 60.0 49.8 48.9 48.9 50.0 43.3 41.7 41.3 42.0 40.5 39.3 39.3 40.0 37.9 35.3 35.9 36.3 36.4 34.0 34.6 Minutes 33.8 32.2 31.8 32.0 30.4 29.6 30.0 24.4 20.0 10.0 0.0 Mean 37.21 LSD(0.05) 3.836 Line SE 2.337 CV(%) 1.5 University of Nairobi ISO 9001:2008 12 Certified http://www.uonbi.ac.ke
  • 13. Fig 5. Cooking time of advanced Small Red canning bean lines selected at Kabete Field Station, 2012 60.0 52.4 50.0 41.8 42.3 40.8 39.6 40.5 38.7 38.3 40.0 36.9 35.4 35.4 35.3 35.1 Minutes 33.7 32.5 34.5 31.6 32.0 31.2 28.7 29.8 30.6 30.0 27.1 20.0 10.0 0.0 Mean 37.21 Line LSD(0.05) 3.836 SE 2.337 CV(%) 1.5
  • 14. Conclusions Results of this work showed significant variation in cooking among bean lines across market classes. More than 60 lines from different market classes cooked faster than the industry standard Mexican-142. This genetic variation for cooking time, can combined with drought tolerance, resistance to diseases and other agronomic traits. Such lines will save time, energy for rural and urban poor in cities and processors. A broad range of Consumer preferences can be met. Increased utilization of beans and income for farmers. University of Nairobi ISO 9001:2008 14 Certified http://www.uonbi.ac.ke
  • 15. Acknowledgements The University of Nairobi Bio-Innovate-Africa program Beans for Climate Change Consortium Supervisors Bean Program Technical staff-UON Governments of Kenya, Sweden-Sida and Somalia University of Nairobi ISO 9001:2008 15 Certified http://www.uonbi.ac.ke
  • 16. THANK YOU University of Nairobi ISO 9001:2008 16 Certified http://www.uonbi.ac.ke