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Safe maize for Africa:
Capacity and Action for Aflatoxin Reduction in Eastern Africa (CAAREA)
BecA‐CSIRO Partnership Flagship Project
Phase I (2011‐2014) Project: Capacity and Action for Aflatoxin 
Reduction in Eastern Africa (CAAREA)
1. Establish a shared mycotoxin/nutritional analysis research platform at the BecA‐ILRI Hub, including gold 
standard and novel aflatoxin diagnostics: BSL2 mycology lab, milling room, cold rooms; LCMS/UPLC, VICAM, 
ELISA, GCMS, AAS, UVVIS, NIR. Used by almost 50 researchers since 2011, multiple hosted projects, open for 
NARS researchers and their partners.
2. Characterize maize fungi from around Kenya and Tanzania: information, biobank and inoculum (see 3). Hosted 
NARS projects also characterized fungi from Burundi, Cameroon, Ethiopia, Kenya, Tanzania, and others, using 
same analyses – comparable for regional comparison.
3.   Identify maize germplasm resistant to aflatoxin accumulation (G x E x M) through first inoculated field trials in 
the region (led by: Kenya – KARI; Tanzania – ARI‐Uyole/Open University of Tanzania): a total of 14 field trials 
conducted (at 7 locations over 3 years, not every trial at every site each year).
4. Test modelling as a potential predictive tool and to contextualize findings regionally (risk maps, process 
models): APSIM model to predict maize aflatoxin risk, on farm survey to characterize maize cropping systems 
(management practices – planting to consumption, maize genotype mapping – GBS, multiple mycotoxin analysis 
by LCMS at BecA and QAAFI).
A shared mycotoxin and nutritional analysis research and capacity building platform has been established at the BecA‐ILRI Hub in Kenya, with funding from the Australian Government and 
scientific involvement of researchers from Kenya, Tanzania, Australia, USA and South Africa. The multinational, multidisciplinary BecA‐CSIRO aflatoxin project team is conducing research that 
has provided key insights and tools for reducing aflatoxins in the region, and will be piloting integrated sets of interventions with smallholder farmers, and actors along the formal maize 
value chain as part of its upcoming second phase. Since it was established in 2010, the platform has hosted almost 50 researchers, including NARS scientists from seven countries, 
researchers from Australia and the USA, and a number of international research centers. The Australian Government Department of Foreign Affairs and Trade is continuing funding for this 
project through 2018.
Project Team and Partners
Phase I and new to Phase II
Funding:
Australian Government, Department of Foreign Affairs and Trade (DFAT) – formerly Australian Agency for International 
Development (AusAID).
Tesfaye Legesse, Paul Greener and others: various project input
Project Leader: Jagger Harvey, Senior Scientist, BecA‐ILRI Hub, j.harvey@cgiar.org
BecA‐ILRI Hub: 
Jagger Harvey (Project Leader, geneticist); Robert Ngeno (lab manager, analytical chemist); James Wainaina, Immaculate Wanjuki, Fredrick Nganga, Sundy
Akello, Eric Magembe and others (research assistants); Benoit Gnonlonfin (postdoc, mycologist); Samuel Mutiga (Cornell PhD student); Warwick Turner 
(analytical chemist, also QDAFF); Boniface Muganda (database systems); Ethel Makila (communications); Jacquiline Mayira, Rachael Mwangi, Berine Ada, 
Rachel Njunge, Valerian Aloo, Josephine Birungi, Timothy Kingori, Agnes Mburu and many others (administrative and logistical support); Appolinaire Djikeng
(BecA Director, BecA‐CSIRO Partnership Leader, strategic project input).
Kenya
Kenya Agricultural and Livestock Research Organization (KALRO; formerly KARI): James Karanja (national maize breeder), Charles Kariuki (entomologist), 
James Gethi, KARI Katumani; Anne Gichangi (agricultural economist) KARI Njoro; Festus Murithi (socioeconomist), and teams.
Tanzania
Agricultural Research Institute ‐ Uyole: Arnold Mushongi (national maize breeder) and team
Ministry of Agriculture and Food Security: Deogratias Lwezaura (agricultural economist) and team
Open University of Tanzania: Said Massomo (plant  pathologist), Fabian Manoza (MSc student), and others.
Nelson Mandela African Institute of Science and Technology (NMAIST): Martin Kimanya
Australia
CSIRO: Ross Darnell (biometrician, Australia project coordinator); Nai Tran‐Dinh (mycologist); Stephen Trowell and Amalia Berna (biosensor technology); Larelle
Macmillan (communications); Peter Carberry and Bruce Pengelly (BecA‐CSIRO Partnership Leader, strategic project input including modelling); Andrew Hall 
(innovation systems); Mario Herrero (household modelling)
CSIRO/HarvestChoice: Darren Kriticos (ecological modeller)
University of Queensland/Queensland Alliance for Agriculture and Food Innovations (QAAFI): Mary Fletcher (natural product organic chemist); Glen Fox (NIR 
expert); Titilayo Falade and Ben Temba (PhD students).
Queensland Department of Agriculture, Forestry and Fisheries (QDAFF): Yash Chauhan (APSIM modelling); Warwick Turner (analytical chemist)
Diversity Arrays Technology Pty Ltd: Andrzej Kilian (genomics)
USA
Cornell University: Rebecca Nelson (molecular plant pathologist); Michael Milgroom (population biologist)
Helica Biosystems Inc: Wondu Wolde‐Mariam – subsidized rates on kits, and technical advice.
HarvestChoice/University of Minnesota: Phil Pardey (agricultural economist), Jason Beddow (postdoc, applied economics), Noboru Ota (GIS input).
South Africa
University of Pretoria/HarvestChoice: Frikkie Liebenberg (survey coordination)
Development Partners:
Farm Input Promotions Africa (FIPS): David Priest and team
Kenya Cereal Millers Association (CMA): Paloma Fernandez and others; engaged in Phase I
Partnership for Aflatoxin Control in Africa (PACA): Amare Ayelew and team; engaged in Phase I.
Phase II (2011‐2014) Project: Aflatoxin Action Alliance
Purpose: Researchers, private and public sector actors, women and men farmers and civil society 
collaboratively develop and apply new knowledge and innovations that improve the accessa nd
availability of aflatoxin‐safe maize.
Outcomes:
1) Farmers, millers and others have improved access and availability of aflatoxin‐safe maize.
2) Participating scientists and institutions incorporate adoption partners and pathways in 
bioscience design and implementation.
Project components:
1. Continue development and improvement of aflatoxin tools and information for use in piloting 
aflatoxin reduction strategies along value chains: sampling and diagnostics, decontamination 
and sorting, modelling‐based decision support tools (process APSIM, risk mapping, and 
hybrid statistical, and household models to identify risk factors, model their potential impact 
on aflatoxin reduction).
2. Engagement of key actors in small, focused innovation platforms (Aflatoxin Action Alliances) 
to consider and discuss information and new findings/tools and their use for reducing 
aflatoxin along value chains, forming new institutional and other collaborations to address 
key agenda issues.
3. Pilot deployment of integrated aflatoxin intervention strategies (information, practices and 
tools) with subsistence farmers. Includes matching varieties to environments in which they 
have lower risk of aflatoxin accumulation, appropriate pre‐harvest management, drying and 
storage practices and tools, and on farm/commercial mill testing and decontamination. 
Objective: pilot interventions with at least 30,000 farmers in high risk areas in Kenya and 
Tanzania.
4. Pilot deployment of aflatoxin intervention strategies (information, testing) along small, pilot 
formal maize value chain. Includes characterizing reduction of aflatoxins in the food supply, 
and behavioral economics to assess affect of interventions with different actors along the 
chain to devise sustainable and effective approaches.
Project team, 2013 Annual Project Workshop Engaging with the BecA aflatoxin platform
Capacity building: The BecA‐ILRI Hub was established to build capacity and complement the strengths of African 
scientists working on key constraints to African agriculture. The aflatoxin team can provide advice, contacts, technical 
protocols, and other information.
Hosting research projects: The mycotoxin, nutritional analysis and broader BecA‐ILRI Hub research platforms were 
established to host research projects focused on African agricultural improvement. A range of projects have already 
been hosted at the mycotoxin‐nutritional analysis platform, including NARS projects from seven sub‐Saharan African 
countries, Australia, USA, and a number of international research institutes.
Research tools and information: Research outputs from the Australia‐funded aflatoxin project include information 
on sampling and testing procedures suited to different contexts, on farm and village level maize mycotoxin surveys, 
Agriculture Production Simulation (APSIM) models for predicting aflatoxin risk for different maize varieties, and 
others.
Funding opportunities: The BecA Africa Biosciences Challenge Fund (ABCF) is a competitive fund available to African 
researchers, to support a 4‐9 month research placement in our laboratories. We receive a large number of 
applications focused on mycotoxin and nutritional analysis research; you are encouraged to apply, and to be as 
specific as possible in your objectives and experimental workplan.
Policy and other stakeholder information: The aflatoxin team has published a policy brief and will continue to 
provide distilled information of use to policymakers and other key stakeholders, upon whom we rely for 
transformative change to address the spectre of aflatoxins in the region.
.
Garissa
Kakamega
Kisii
Kisumu
Kitale
Kiboko
Makindu
Lamu
Malindi
Mandera
Marsabit
Meru
Mombasa
Moyale
Nairobi
Nakuru
Narok
Nyeri
Lodwar
Voi
Wajir
Katumani
Aflatoxinriskindex
100
80
60
40
20
0
Aflatoxin risk for variety X
Farm locality
This document is licensed for use under a Creative Commons Attribution‐Noncommercial‐Share A
like 3.0 Unported License        October  2014

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Safe maize for Africa: Capacity and Action for Aflatoxin Reduction in Eastern Africa (CAAREA)

  • 1. Safe maize for Africa: Capacity and Action for Aflatoxin Reduction in Eastern Africa (CAAREA) BecA‐CSIRO Partnership Flagship Project Phase I (2011‐2014) Project: Capacity and Action for Aflatoxin  Reduction in Eastern Africa (CAAREA) 1. Establish a shared mycotoxin/nutritional analysis research platform at the BecA‐ILRI Hub, including gold  standard and novel aflatoxin diagnostics: BSL2 mycology lab, milling room, cold rooms; LCMS/UPLC, VICAM,  ELISA, GCMS, AAS, UVVIS, NIR. Used by almost 50 researchers since 2011, multiple hosted projects, open for  NARS researchers and their partners. 2. Characterize maize fungi from around Kenya and Tanzania: information, biobank and inoculum (see 3). Hosted  NARS projects also characterized fungi from Burundi, Cameroon, Ethiopia, Kenya, Tanzania, and others, using  same analyses – comparable for regional comparison. 3.   Identify maize germplasm resistant to aflatoxin accumulation (G x E x M) through first inoculated field trials in  the region (led by: Kenya – KARI; Tanzania – ARI‐Uyole/Open University of Tanzania): a total of 14 field trials  conducted (at 7 locations over 3 years, not every trial at every site each year). 4. Test modelling as a potential predictive tool and to contextualize findings regionally (risk maps, process  models): APSIM model to predict maize aflatoxin risk, on farm survey to characterize maize cropping systems  (management practices – planting to consumption, maize genotype mapping – GBS, multiple mycotoxin analysis  by LCMS at BecA and QAAFI). A shared mycotoxin and nutritional analysis research and capacity building platform has been established at the BecA‐ILRI Hub in Kenya, with funding from the Australian Government and  scientific involvement of researchers from Kenya, Tanzania, Australia, USA and South Africa. The multinational, multidisciplinary BecA‐CSIRO aflatoxin project team is conducing research that  has provided key insights and tools for reducing aflatoxins in the region, and will be piloting integrated sets of interventions with smallholder farmers, and actors along the formal maize  value chain as part of its upcoming second phase. Since it was established in 2010, the platform has hosted almost 50 researchers, including NARS scientists from seven countries,  researchers from Australia and the USA, and a number of international research centers. The Australian Government Department of Foreign Affairs and Trade is continuing funding for this  project through 2018. Project Team and Partners Phase I and new to Phase II Funding: Australian Government, Department of Foreign Affairs and Trade (DFAT) – formerly Australian Agency for International  Development (AusAID). Tesfaye Legesse, Paul Greener and others: various project input Project Leader: Jagger Harvey, Senior Scientist, BecA‐ILRI Hub, j.harvey@cgiar.org BecA‐ILRI Hub:  Jagger Harvey (Project Leader, geneticist); Robert Ngeno (lab manager, analytical chemist); James Wainaina, Immaculate Wanjuki, Fredrick Nganga, Sundy Akello, Eric Magembe and others (research assistants); Benoit Gnonlonfin (postdoc, mycologist); Samuel Mutiga (Cornell PhD student); Warwick Turner  (analytical chemist, also QDAFF); Boniface Muganda (database systems); Ethel Makila (communications); Jacquiline Mayira, Rachael Mwangi, Berine Ada,  Rachel Njunge, Valerian Aloo, Josephine Birungi, Timothy Kingori, Agnes Mburu and many others (administrative and logistical support); Appolinaire Djikeng (BecA Director, BecA‐CSIRO Partnership Leader, strategic project input). Kenya Kenya Agricultural and Livestock Research Organization (KALRO; formerly KARI): James Karanja (national maize breeder), Charles Kariuki (entomologist),  James Gethi, KARI Katumani; Anne Gichangi (agricultural economist) KARI Njoro; Festus Murithi (socioeconomist), and teams. Tanzania Agricultural Research Institute ‐ Uyole: Arnold Mushongi (national maize breeder) and team Ministry of Agriculture and Food Security: Deogratias Lwezaura (agricultural economist) and team Open University of Tanzania: Said Massomo (plant  pathologist), Fabian Manoza (MSc student), and others. Nelson Mandela African Institute of Science and Technology (NMAIST): Martin Kimanya Australia CSIRO: Ross Darnell (biometrician, Australia project coordinator); Nai Tran‐Dinh (mycologist); Stephen Trowell and Amalia Berna (biosensor technology); Larelle Macmillan (communications); Peter Carberry and Bruce Pengelly (BecA‐CSIRO Partnership Leader, strategic project input including modelling); Andrew Hall  (innovation systems); Mario Herrero (household modelling) CSIRO/HarvestChoice: Darren Kriticos (ecological modeller) University of Queensland/Queensland Alliance for Agriculture and Food Innovations (QAAFI): Mary Fletcher (natural product organic chemist); Glen Fox (NIR  expert); Titilayo Falade and Ben Temba (PhD students). Queensland Department of Agriculture, Forestry and Fisheries (QDAFF): Yash Chauhan (APSIM modelling); Warwick Turner (analytical chemist) Diversity Arrays Technology Pty Ltd: Andrzej Kilian (genomics) USA Cornell University: Rebecca Nelson (molecular plant pathologist); Michael Milgroom (population biologist) Helica Biosystems Inc: Wondu Wolde‐Mariam – subsidized rates on kits, and technical advice. HarvestChoice/University of Minnesota: Phil Pardey (agricultural economist), Jason Beddow (postdoc, applied economics), Noboru Ota (GIS input). South Africa University of Pretoria/HarvestChoice: Frikkie Liebenberg (survey coordination) Development Partners: Farm Input Promotions Africa (FIPS): David Priest and team Kenya Cereal Millers Association (CMA): Paloma Fernandez and others; engaged in Phase I Partnership for Aflatoxin Control in Africa (PACA): Amare Ayelew and team; engaged in Phase I. Phase II (2011‐2014) Project: Aflatoxin Action Alliance Purpose: Researchers, private and public sector actors, women and men farmers and civil society  collaboratively develop and apply new knowledge and innovations that improve the accessa nd availability of aflatoxin‐safe maize. Outcomes: 1) Farmers, millers and others have improved access and availability of aflatoxin‐safe maize. 2) Participating scientists and institutions incorporate adoption partners and pathways in  bioscience design and implementation. Project components: 1. Continue development and improvement of aflatoxin tools and information for use in piloting  aflatoxin reduction strategies along value chains: sampling and diagnostics, decontamination  and sorting, modelling‐based decision support tools (process APSIM, risk mapping, and  hybrid statistical, and household models to identify risk factors, model their potential impact  on aflatoxin reduction). 2. Engagement of key actors in small, focused innovation platforms (Aflatoxin Action Alliances)  to consider and discuss information and new findings/tools and their use for reducing  aflatoxin along value chains, forming new institutional and other collaborations to address  key agenda issues. 3. Pilot deployment of integrated aflatoxin intervention strategies (information, practices and  tools) with subsistence farmers. Includes matching varieties to environments in which they  have lower risk of aflatoxin accumulation, appropriate pre‐harvest management, drying and  storage practices and tools, and on farm/commercial mill testing and decontamination.  Objective: pilot interventions with at least 30,000 farmers in high risk areas in Kenya and  Tanzania. 4. Pilot deployment of aflatoxin intervention strategies (information, testing) along small, pilot  formal maize value chain. Includes characterizing reduction of aflatoxins in the food supply,  and behavioral economics to assess affect of interventions with different actors along the  chain to devise sustainable and effective approaches. Project team, 2013 Annual Project Workshop Engaging with the BecA aflatoxin platform Capacity building: The BecA‐ILRI Hub was established to build capacity and complement the strengths of African  scientists working on key constraints to African agriculture. The aflatoxin team can provide advice, contacts, technical  protocols, and other information. Hosting research projects: The mycotoxin, nutritional analysis and broader BecA‐ILRI Hub research platforms were  established to host research projects focused on African agricultural improvement. A range of projects have already  been hosted at the mycotoxin‐nutritional analysis platform, including NARS projects from seven sub‐Saharan African  countries, Australia, USA, and a number of international research institutes. Research tools and information: Research outputs from the Australia‐funded aflatoxin project include information  on sampling and testing procedures suited to different contexts, on farm and village level maize mycotoxin surveys,  Agriculture Production Simulation (APSIM) models for predicting aflatoxin risk for different maize varieties, and  others. Funding opportunities: The BecA Africa Biosciences Challenge Fund (ABCF) is a competitive fund available to African  researchers, to support a 4‐9 month research placement in our laboratories. We receive a large number of  applications focused on mycotoxin and nutritional analysis research; you are encouraged to apply, and to be as  specific as possible in your objectives and experimental workplan. Policy and other stakeholder information: The aflatoxin team has published a policy brief and will continue to  provide distilled information of use to policymakers and other key stakeholders, upon whom we rely for  transformative change to address the spectre of aflatoxins in the region. . Garissa Kakamega Kisii Kisumu Kitale Kiboko Makindu Lamu Malindi Mandera Marsabit Meru Mombasa Moyale Nairobi Nakuru Narok Nyeri Lodwar Voi Wajir Katumani Aflatoxinriskindex 100 80 60 40 20 0 Aflatoxin risk for variety X Farm locality This document is licensed for use under a Creative Commons Attribution‐Noncommercial‐Share A like 3.0 Unported License        October  2014