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L.O: students will review laboratory
techniques and the four required labs.
First lab safety:

You should know what to do in a
          lab…and……
You should know what NOT to do!
You will have ONE question on lab
   techniques and lab safety!
                      Are you being safe?
Be able toexplain why any one of these
        kids is not being safe”!
             Are you being safe?
Measurements: when
 one makes an observation
using numbers & a measuring
        instrument.
    There will be one measurement
       question on the regents!
Example: what is the exact
measurement of this worm?
9.0 centimeters
Example 2: how much water is in the
       graduated cylinder?
13.0 mL.
Example 3: how much water is in the
       graduated cylinder?
24.0 mL
Now answer 1-6.
Microscopes:
      • You should know the
        basic parts of a
        microscope.
      • There may be one
        question.
Microscopes are use to make small
       objects look bigger.
Objects under a microscope are always
    upside down and backwards.
Because objects are upside down and
  backwards, one has to move a slide
 the OPPOSITE of where one wants to
          move an object.
                           I must move the
                           slide this way




If i want to move the
organism this way
When wet-mounting a specimen on a
slide, the cover-slip must be lowered
 at an angle to prevent air bubbles.
To stain a specimen, place a paper towel at
one side of the slip cover. Add the dye stain
 at the opposite end. Capillary action will
    “pull” the dye through, staining the
                 specimen.
A microscope’s field of view is the diameter
  of the microscope’s circle. Knowing the
field of view can be used to determine the
           size of microorganisms.
If this microscope’s field of view under low
  power is 1000um, what is the size of the
                 organism?




  1000um
If this microscope’s field of view under low
  power is 1000um, what is the size of the

       organism?   ~200um

  1000um
Now answer 7-14.
And now the four required labs.
We did a diffusion through a membrane
                   lab
We did a making connections lab
We did a relationships & biodiversity lab
We did a beaks of finches lab
First the diffusion through a membrane
                  lab>>>
The goal of the lab is to model how a cell
 uses diffusion to maintain homeostasis.
1. We put starch and
What we did:      glucose inside a piece of
                  plastic called dialysis
                  tubing.
               2. We tied both ends tight
                  with string.
               3. We place several drops
                  of iodine starch indicato
                  into a cup of water.
We placed the dialysis tube in cup to
        see what happens:
• The           will
Our predictions:                    of the
                     dialysis tube because
                     the
                           .
                   • The         will
                           the tube because
                     the
                           .
                   • The         will stay
                     inside the tube because
                     the                   .

                                          .
Results:
The starch molecules were too large to diffuse out. Inside
dialysis tube turned blue-black (purple-black) because the
 starch indicator (iodine) diffused into tube & change the
                       starches’ color.
A sample of the liquid from outside the
tubing turned glucose indicator orange
 because the small glucose diffuse out.
Indicators:
Indicators are used to determine if a
substance (ex. Starch or glucose) is present
                 (is there).
Starch indicator (iodine) turns purple-black
       if Starch is present (is there).
Glucose indicator turns orange if glucose is
            present (is there).
Diffusion: the movement of molecules
 from areas of high concentration to
 areas of low concentration without
             using energy.
Diffusion: the movement of molecules
 from areas of high concentration to areas
of low concentration without using energy.

                                                  More          Less
                                                  sugar        sugar
                  More
                  sugar              Less sugar
                   97%



                                                    Cell has   Surrounding has
                                                      97%      92% water
                                                     water
                          Cell has                   inside
    surrounding
                            94%
    Area has
                           water
     96% water
                           inside
Diffusion: molecules move from high
 concentration to low concentration
        without using energy.

                                               More          Less
                                               sugar        sugar
               More
               sugar              Less sugar
                97%



                                                 Cell has   Surrounding has
                                                   97%      92% water
                                                  water
                       Cell has                   inside
 surrounding
                         94%
 Area has
                        water
  96% water
                        inside
Diffusion: molecules move from high
 concentration to low concentration
        without using energy.




                          Cell has   Surrounding has
                           100%      6% water
                           water
 surrounding   Cell has    inside
 Area has      6% salt
  100% water    inside
Diffusion molecules move from areas
of high concentration to areas of low
           concentration.
Diffusion molecules move from areas
of high concentration to areas of low
           concentration.
Why will easy for molecule B diffuse
into the cell but hard for molecule A?
Another part of the diffusion through
 a membrane dealt with red onion
                cells.
What you did:
we looked at slices of red onions, then….
Salt water was added to the onion slices.



                Salt
               water
First we saw view A: normal red onion
                  cells.
After the salt solution: the onion cells
               shriveled.
Then we put distilled (zero salt) water
 on the shriveled cells… they swelled
Remember: salt solutions cause cells to
 shrivel. Distilled water causes cells to
                   swell.
Now the beaks of finches lab:



                           Darwin’s
                           finches
Know how to read and use this chart:
The outer circle has the names and
      drawings of the bills.
The next two circles describe the type
     of the bills each species has.
The inner circle shows the type of the
       food each species eats.
This is another finch chart that has
appeared on previous regents exams.
Now answer 15-23.
Gel
electrophoresis:
Gel electrophoresis is a method of
analyzing DNA (also RNA & proteins)
The DNA (or RNA & proteins) are cut
         using enzymes…
The DNA (or RNA & proteins) samples are
 put into wells on the gel and an electric
 current is run. The electricity makes the
             DNA bands more
Uses of gel electrophoresis:
1. Evolutionary relationships: if organisms
               are related.



Bands of
DNA
2. Gel electrophoresis can be
used to determine paternity
 Who the parent or who the relative
                is.
3. Gel electrophoresis can be used for
genetic testing. For genetic disorders
4. To solve crimes using DNA
Gel electrophoresis separates the DNA
 bands by size. The smallest move fastest
             through the gel.



Bands of
DNA
The DNA results from gel
electrophoresis is often used to make
         claudogram trees!
Gel electrophoresis is 99% or more
accurate…. It is how we know humans
 are related to the other great apes.
Gel electrophoresis is 99% or more
accurate…. It is how we know humans
  are related to the other great apes
It is how we know if someone is guilty
        or innocent of a crime….
It is how we know who the “baby
    daddy” is on Maury or Jerry Springer!
You ARE
   the
 father!
In the Relationships and Biodiversity lab we
were looking for which plant was related to
     BotanaCurus, a hypothetical cancer
                 treatment.
The plants has similar appearance, seeds & microscope slides…
We used gel electrophoresis (DNA &
Amino acids) to determine which plant was
         related to BotanaCurus.
We used gel electrophoresis (DNA &
Amino acids) to determine which plant was
         related to BotanaCurus.
Remember: DNA (gel electrophoresis) &
amino acids are the most accurate way to
   identify or determine relationships.
Which the most closely related to A,
  according the the DNA bands?
Which the most closely related to A,
  according the the DNA bands?
Which organisms are the most closely
related according to their amino acids?
Now answer: 24- 46.
Paper chromatography:
Chromatography: a method of
separating different molecules in a
             mixture.
The final mandatory lab is the
  making connections lab
The human body is like a machine
 with many complicated systems.
  All the systems must work together
        to maintain homeostasis.
All of our organ systems work together
      to maintain our homeostasis.
               Examples:
• Our muscular system moves our body. It gets
  Oxygen from our respiratory system in order
  to produce ATP. The circulatory system brings
  the oxygen to the muscles. Our excretory
  system sweats to keep the muscles cool (from
  over heating).
When we’re very active. All of our
systems speed-up to keep up with the
         increased activity.
Why does running cause more exhaled
          air than sitting?
Sample regents question
Sample regents question
Sample regents question
Sample regents question
Sample regents question
Now complete all the remaining
        questions.

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