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TNJFU REFERRAL LAB FOR AQUATIC ANIMAL HEALTH
TNJFU Headquarters
Nagapattinam
TNJFU REFERRAL LAB FOR AQUATIC ANIMAL HEALTH
•Lab was initiated during the year 6th February 2018
•Used for sample analysis from Water/Animal/Soil/ feed
•Used for Practical classes from CoFE, Nagapattinam
List of Equipments
Sl.No Description Numbers
1. Refractometer 1 No
2. pH Meter 2 No
3. All Quartz Double Distillation Unit 1 No
4. Water Bath 1 No
5. Island Table 1 set
6. Water softener 1 No
7. Hot air oven 2 No
8. Distillation unit (Heat exchanger) 1 No
9. Laminar flow horizontal 2 Nos
10. Hot plate 1 No
11. Vacuum pressure pump 1 No
12. Analytical balance 3 Nos
13. Kel plus automatic dual distillation 1 No
14. High speed cooling centrifuge 1 No
15. Incubator 4 Nos
16 Autoclave 2 Nos
Sl.No Description Numbers
17. Muffle furnace 1 No
18. Bod incubator 1 No
19. Spectrophotometer 1 No
20. Flame photometer 1 No
21. Kelplus kelvac digestion unit – pelican 1 No
22. Petri dish carrier with handle 1 No
23. Hand held digital colony counter 1 No
24. Manual pipette 1 No
25. Auto pipette 1 No
26. KES Series Automatic Macro Block Digestion 1 No
27. Multiprobe system make Hanna equipment accessories 1 No
28. Soxplus Automatic pc compatible systems (pelican) 1 No
29. Fibraplus Automatic pc compatible fiber estimation systems pelican 1 No
30. Split air conditioner capacity 1.5 ton 1 No
31. Online UPS 15 KVA 2 Nos
32. Inverted light microscope & accessories 1 No
Sl.No Description Numbers
33. Magnetic Stirrer 2 No
34. Cooling centrifuge Angle Head 1 No
35. Biophotometer 1 No
36. Refrigerated Mini Centrifuge 1 No
37. Thermal Cycler 1 No
38. Gel Doc with accessories 1 No
39. Electrophorosis and Power Pac 1 No
40. Desktop Computer 2 Nos
41. Laptop 1 No
42. Printer 1 No
43. Deep Freezer Horizontal – 200C 1 No
44. vortex mixer 1 No
45. Lab Stool 8 Nos
46. Refrigerator 1 No
47. Microwave oven 2 Nos
48. 10 KVA Online UPS 1 No
49. Air Conditioner – 5 ton 2 Nos
50. Digital Heating block - DBK 1 No
51 Bio Safety cabinet 1 No
Sl.No Description Numbers
52. Mini Refrigerator 1 No
53. Digital Turbidity Meter 1 No
54. Deep Freezer - 40° C 1 No
55. Mobile laboratory (Force traveller van) UPS 3 KVA (3 Batteries) 1 No
56. UV Cabinet with Transilluminator Protection (for Mobile laboratory
van)
1 No
57. Mini horizontal electrophoresis with gel caster (for Mobile
laboratory van)
1 No
58. Power pack gel electrophoresis (for Mobile laboratory van) 1 No
59. Bench top mini cooler (for Mobile laboratory van) 2 Nos
60. Multiparameter hand held water quality meter (for Mobile
laboratory van)
3 Nos
61. Dissolved Oxygen meter (for Mobile laboratory van) 1 No
62. Portable Turbidity meter (for Mobile laboratory van) 1 No
63. Microcentrifuge mini spin (for Mobile laboratory van) 1 No
64. Soil test photometer kit 1 No
List of parameters testing in lab
• 1. water samples
• 2. Soil samples
• 3. Feed samples
• 4. Animals ( Fish & Shrimp) samples
Parameters testing in water samples
Sl.No Parameters
1. pH
2. Hardness
3. Total Alkalinity
4. Salinity by titration
5. Dissolved Oxygen (DO)
6. Biological Oxygen Demand (BOD)
7. Biological Oxygen Demand (BOD) for waste water
8. Chemical Oxygen Demand (COD)
9. Chemical Oxygen Demand (COD) for waste water
10. Hytrogen sulphide (H2S)
11. Nitrite (NO2)
12. Nitrate (NO3)
13. Ammonia
Sl.No Parameters
14. Phosphate
15. Total Dissolved Solids (TDS)
16. Total Suspended Solids (TSS)
17 Magnesium by titration
18 Calcium by titration
19. Iron
20. Sodium
21. Potassium
22. Electrical Conductivity
23. Turbidity
24. Fluoride
25. Chloride
26. Residual chlorine
27. Total Plate Count
28. Total vibrio count
29. Phytoplankton
30. Zooplankton
Parameters testing in Soil samples
Sl.No Parameters
1. pH
2. Available Nitrogen
3. Available phosphate
4. Total Organic Carbon (TOC)
5. Total Organic Nitrogen (TON)
6. Water holding capacity
7. Electrical conductivity (EC)
8. Phosphorus
9. Potassium
10. Magnesium
11. Calcium
12. Copper
13. Iron
14. Manganese
15. Sulfate
Parameters testing in Feed samples
Sl.No Parameters
1. Moisture
2. Crude protein
3. Crude Fat
4. Total Ash
5. Crude fibre
6. Total Acid Insoluble Ash
7. Total carbohydrate
Parameters testing in Animals (Fish & Shrimp) samples
Sl.No Parameters
1. Moisture
2. Crude protein
3. Crude Fat
4. Total Ash
5. Crude fibre
6. Total Acid Insoluble Ash
7. Total Plate Count
8. Total vibrio count
9. Real time PCR Diagnosis for Aquatic Animals
10. Stress Test / fry Health analysis
11. Antibiotic sensitivity Test
12. White spot virus (WSSV) by PCR
13. Total Aerobic Bacterial Count
14. Prescence of parasites by microscopy
15. Enterocytozoan Hepatopenaei (EHP) by PCR method
Samples analysed & revenue earned – year 2017-2018
Sl.No Month Number of sample Amount in Rs.
1.
February
Soil samples - 16 2000
Water samples - 15 3000
2. March Water samples -1 200
Total revenue 5,200
Total No. of Water Samples = 16 Rs: 3,200/-
Total No. of Soil Samples = 16 Rs: 2,000/-
Total No. of Samples = 32
Revenue: Rs. 5,200/-
April 2018 to March 2019
Sl.No Month
Water
samples
Soil samples
Feed
samples
1 Apr- 2018 13 - -
2 May – 18 10 9 10
3 June – 18 13 13 -
4 July – 18 13 6 -
5 Aug – 18 31 33 -
6 Sep – 18 14 7 -
7 Oct – 18 13 - -
8 Nov – 18 3 - -
9 Dec – 18 4 - 1
10 Jan – 19 10 - 1
11 Feb – 19 10 - 3
12 March - 2019 14 - 10
Total 148 68 25
Total No. of Water Samples = 148 Rs: 29,600/-
Total No. of Soil Samples = 68 Rs: 6800/-
Total No. of Feed Samples = 25 Rs: 10,000/-
Total No. of Samples = 241
Revenue: Rs. 46,400/-
April 2018 to March 2019
April 2019 to March 2020
Si.No Month Water
Sample
soil
samples
Fish
Sample
Feed
Samples
1 APRIL - 2019 43 - - -
2 MAY 11 32 1 4
3 JUNE 8 1 5 -
4 JULY 65 1 - -
5 AUG 14 6 - -
6 SEP 2 - - -
7 OCT 11 - - -
8 NOV 12 - - -
9 DEC 3 - - -
10 JAN - 2020 1 1 - -
11 FEBRUARY 1 - - -
12 MARCH 15 4 - -
Total 186 45 6 4
Total No. of Water samples= 186 Rs: 37050
Total No. of Soil samples = 45 Rs: 4500
Total No. of Fish samples = 6 Rs: 2400
Total No. of Feed Samples = 4 Rs: 1600
Total No. of Samples = 241
Revenue : Rs.45,550/-
April 2019 to March 2020
April 2020 to March 2021
Month Water
Samples
Soil
Samples
Feed
Samples
Animals
(shrimp &
Fish)
No. of
Samples
April – 2020 - - - - -
May 6 4 - 1 11
June 39 25 16 80
July 6 - - 6
Aug 29 1 - 30
Sep 6 1 - 7
Oct 33 - - 33
Nov 4 - - 4
Dec 3 - - 3 6
Jan – 2021 7 - 3 3 13
Feb 9 9 - - 18
March 1 - - - 1
Total 143 40 19 7 209
Total No. of Water samples = 143 Rs:28,600
Total No. of Soil samples = 40 Rs:4000
Total No. of Animals samples = 7 Rs: 2800
Total No. of Feed Samples = 19 Rs:7600
Revenue: Rs. 43,000/-
April 2020 to March 2021
April 2021 to March 2022
Month Water
Samples
Soil
Samples
Feed
Samples
Animals
(shrimp & Fish)
No. of
Samples
April – 2021 2 - 8 - 10
May - - - - -
June - - - - -
July 1 2 - 3
Aug 48 - - 4 52
Sep 120 - - 1 121
Oct - - - - -
Nov 1 - - 2 3
Dec 35 - 1 - 36
Jan – 2022 2 1 - - 3
Feb 4 - - - 4
March 12 - - - 12
Total 225 3 9 7 244
Total No. of Water samples = 225 Rs: 189380
Total No. of Soil samples = 3 Rs: 2100
Total No. of Animals samples = 7 Rs: 4220
Total No. of Feed Samples = 9 Rs: 4760
Total No. of Samples = 244
Revenue: Rs. 2,00,460/-
April 2021 to March 2022
April 2022 to February 2023
Month Water
Samples
Soil
Samples
Feed
Samples
Animals
(shrimp & Fish)
No. of
Samples
April – 2022 111 - - - 111
May 07 - - - 07
June 26 - - 01 27
July 27 7 - - 34
Aug - - - - -
Sep 25 - - - 25
Oct 40 30 - - 70
Nov - - - - -
Dec - - - - -
January - 2023 02 - - - 02
Feb 02 05 - - 07
TOTAL 283
April 2022 to February 2023
Total No. of Water samples = 240 Rs: 147775
Total No. of Soil samples = 42 Rs: 9700
Total No. of Animals samples = 1 Rs: 650
Total No. of samples = 283
Revenue: Rs: 169105
TANII – 2017-2020
• E-Fish health surveillance and monitoring network to improve
fisheries production in Tamil Nadu. (Sc.No.XV-102-04)
Total Budget : Rs. 59.4 lakhs
Duration : Three years
Date of start : 23.03.2017
Date of closure
(tentative, if it is
ongoing)
: 30.09.2020
Expenditure
2021-2022
• Expenditure details on TNJFU Referral lab for the year of 2021-2022
Recurring expenses : Rs. 83,768/-
• Manpower expenses: 2,86,500/-
2022-2023
• Expenditure details on TNJFU Referral lab for the year of 2022-2023 (February)
• Recurring expenses : Rs. 5,500/-
• Manpower expenses: 2,85,000/-
Cost Benefit Analysis
2021-2022
• Total Expenditure : 3,70,268 /-
• Total Revenue : 2,00,460/-
• Cost Benefit Ratio: 0.54
2022-2023
• Total Expenditure : 2,90,500 /-
• Total Revenue : 1,58,125/-
• Cost Benefit Ratio: 0.54
Future Plan:
• To give more publicity to fish and shrimp farmers for sample analysis.
• On spot sample analysis will be carried out.
• Online UPS 10 KVA to be purchased for routine sample analysis.
• Repairing of mobile van to be carried out.
• To apply for NABL Accreditation.

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REFERRAL LAB - 23.02.2023.pptx

  • 1. TNJFU REFERRAL LAB FOR AQUATIC ANIMAL HEALTH TNJFU Headquarters Nagapattinam
  • 2. TNJFU REFERRAL LAB FOR AQUATIC ANIMAL HEALTH •Lab was initiated during the year 6th February 2018 •Used for sample analysis from Water/Animal/Soil/ feed •Used for Practical classes from CoFE, Nagapattinam
  • 3. List of Equipments Sl.No Description Numbers 1. Refractometer 1 No 2. pH Meter 2 No 3. All Quartz Double Distillation Unit 1 No 4. Water Bath 1 No 5. Island Table 1 set 6. Water softener 1 No 7. Hot air oven 2 No 8. Distillation unit (Heat exchanger) 1 No 9. Laminar flow horizontal 2 Nos 10. Hot plate 1 No 11. Vacuum pressure pump 1 No 12. Analytical balance 3 Nos 13. Kel plus automatic dual distillation 1 No 14. High speed cooling centrifuge 1 No 15. Incubator 4 Nos 16 Autoclave 2 Nos
  • 4. Sl.No Description Numbers 17. Muffle furnace 1 No 18. Bod incubator 1 No 19. Spectrophotometer 1 No 20. Flame photometer 1 No 21. Kelplus kelvac digestion unit – pelican 1 No 22. Petri dish carrier with handle 1 No 23. Hand held digital colony counter 1 No 24. Manual pipette 1 No 25. Auto pipette 1 No 26. KES Series Automatic Macro Block Digestion 1 No 27. Multiprobe system make Hanna equipment accessories 1 No 28. Soxplus Automatic pc compatible systems (pelican) 1 No 29. Fibraplus Automatic pc compatible fiber estimation systems pelican 1 No 30. Split air conditioner capacity 1.5 ton 1 No 31. Online UPS 15 KVA 2 Nos 32. Inverted light microscope & accessories 1 No
  • 5. Sl.No Description Numbers 33. Magnetic Stirrer 2 No 34. Cooling centrifuge Angle Head 1 No 35. Biophotometer 1 No 36. Refrigerated Mini Centrifuge 1 No 37. Thermal Cycler 1 No 38. Gel Doc with accessories 1 No 39. Electrophorosis and Power Pac 1 No 40. Desktop Computer 2 Nos 41. Laptop 1 No 42. Printer 1 No 43. Deep Freezer Horizontal – 200C 1 No 44. vortex mixer 1 No 45. Lab Stool 8 Nos 46. Refrigerator 1 No 47. Microwave oven 2 Nos 48. 10 KVA Online UPS 1 No 49. Air Conditioner – 5 ton 2 Nos 50. Digital Heating block - DBK 1 No 51 Bio Safety cabinet 1 No
  • 6. Sl.No Description Numbers 52. Mini Refrigerator 1 No 53. Digital Turbidity Meter 1 No 54. Deep Freezer - 40° C 1 No 55. Mobile laboratory (Force traveller van) UPS 3 KVA (3 Batteries) 1 No 56. UV Cabinet with Transilluminator Protection (for Mobile laboratory van) 1 No 57. Mini horizontal electrophoresis with gel caster (for Mobile laboratory van) 1 No 58. Power pack gel electrophoresis (for Mobile laboratory van) 1 No 59. Bench top mini cooler (for Mobile laboratory van) 2 Nos 60. Multiparameter hand held water quality meter (for Mobile laboratory van) 3 Nos 61. Dissolved Oxygen meter (for Mobile laboratory van) 1 No 62. Portable Turbidity meter (for Mobile laboratory van) 1 No 63. Microcentrifuge mini spin (for Mobile laboratory van) 1 No 64. Soil test photometer kit 1 No
  • 7. List of parameters testing in lab • 1. water samples • 2. Soil samples • 3. Feed samples • 4. Animals ( Fish & Shrimp) samples
  • 8. Parameters testing in water samples Sl.No Parameters 1. pH 2. Hardness 3. Total Alkalinity 4. Salinity by titration 5. Dissolved Oxygen (DO) 6. Biological Oxygen Demand (BOD) 7. Biological Oxygen Demand (BOD) for waste water 8. Chemical Oxygen Demand (COD) 9. Chemical Oxygen Demand (COD) for waste water 10. Hytrogen sulphide (H2S) 11. Nitrite (NO2) 12. Nitrate (NO3) 13. Ammonia
  • 9. Sl.No Parameters 14. Phosphate 15. Total Dissolved Solids (TDS) 16. Total Suspended Solids (TSS) 17 Magnesium by titration 18 Calcium by titration 19. Iron 20. Sodium 21. Potassium 22. Electrical Conductivity 23. Turbidity 24. Fluoride 25. Chloride 26. Residual chlorine 27. Total Plate Count 28. Total vibrio count 29. Phytoplankton 30. Zooplankton
  • 10. Parameters testing in Soil samples Sl.No Parameters 1. pH 2. Available Nitrogen 3. Available phosphate 4. Total Organic Carbon (TOC) 5. Total Organic Nitrogen (TON) 6. Water holding capacity 7. Electrical conductivity (EC) 8. Phosphorus 9. Potassium 10. Magnesium 11. Calcium 12. Copper 13. Iron 14. Manganese 15. Sulfate
  • 11. Parameters testing in Feed samples Sl.No Parameters 1. Moisture 2. Crude protein 3. Crude Fat 4. Total Ash 5. Crude fibre 6. Total Acid Insoluble Ash 7. Total carbohydrate
  • 12. Parameters testing in Animals (Fish & Shrimp) samples Sl.No Parameters 1. Moisture 2. Crude protein 3. Crude Fat 4. Total Ash 5. Crude fibre 6. Total Acid Insoluble Ash 7. Total Plate Count 8. Total vibrio count 9. Real time PCR Diagnosis for Aquatic Animals 10. Stress Test / fry Health analysis 11. Antibiotic sensitivity Test 12. White spot virus (WSSV) by PCR 13. Total Aerobic Bacterial Count 14. Prescence of parasites by microscopy 15. Enterocytozoan Hepatopenaei (EHP) by PCR method
  • 13. Samples analysed & revenue earned – year 2017-2018 Sl.No Month Number of sample Amount in Rs. 1. February Soil samples - 16 2000 Water samples - 15 3000 2. March Water samples -1 200 Total revenue 5,200 Total No. of Water Samples = 16 Rs: 3,200/- Total No. of Soil Samples = 16 Rs: 2,000/- Total No. of Samples = 32 Revenue: Rs. 5,200/-
  • 14. April 2018 to March 2019 Sl.No Month Water samples Soil samples Feed samples 1 Apr- 2018 13 - - 2 May – 18 10 9 10 3 June – 18 13 13 - 4 July – 18 13 6 - 5 Aug – 18 31 33 - 6 Sep – 18 14 7 - 7 Oct – 18 13 - - 8 Nov – 18 3 - - 9 Dec – 18 4 - 1 10 Jan – 19 10 - 1 11 Feb – 19 10 - 3 12 March - 2019 14 - 10 Total 148 68 25
  • 15. Total No. of Water Samples = 148 Rs: 29,600/- Total No. of Soil Samples = 68 Rs: 6800/- Total No. of Feed Samples = 25 Rs: 10,000/- Total No. of Samples = 241 Revenue: Rs. 46,400/- April 2018 to March 2019
  • 16. April 2019 to March 2020 Si.No Month Water Sample soil samples Fish Sample Feed Samples 1 APRIL - 2019 43 - - - 2 MAY 11 32 1 4 3 JUNE 8 1 5 - 4 JULY 65 1 - - 5 AUG 14 6 - - 6 SEP 2 - - - 7 OCT 11 - - - 8 NOV 12 - - - 9 DEC 3 - - - 10 JAN - 2020 1 1 - - 11 FEBRUARY 1 - - - 12 MARCH 15 4 - - Total 186 45 6 4
  • 17. Total No. of Water samples= 186 Rs: 37050 Total No. of Soil samples = 45 Rs: 4500 Total No. of Fish samples = 6 Rs: 2400 Total No. of Feed Samples = 4 Rs: 1600 Total No. of Samples = 241 Revenue : Rs.45,550/- April 2019 to March 2020
  • 18. April 2020 to March 2021 Month Water Samples Soil Samples Feed Samples Animals (shrimp & Fish) No. of Samples April – 2020 - - - - - May 6 4 - 1 11 June 39 25 16 80 July 6 - - 6 Aug 29 1 - 30 Sep 6 1 - 7 Oct 33 - - 33 Nov 4 - - 4 Dec 3 - - 3 6 Jan – 2021 7 - 3 3 13 Feb 9 9 - - 18 March 1 - - - 1 Total 143 40 19 7 209
  • 19. Total No. of Water samples = 143 Rs:28,600 Total No. of Soil samples = 40 Rs:4000 Total No. of Animals samples = 7 Rs: 2800 Total No. of Feed Samples = 19 Rs:7600 Revenue: Rs. 43,000/- April 2020 to March 2021
  • 20. April 2021 to March 2022 Month Water Samples Soil Samples Feed Samples Animals (shrimp & Fish) No. of Samples April – 2021 2 - 8 - 10 May - - - - - June - - - - - July 1 2 - 3 Aug 48 - - 4 52 Sep 120 - - 1 121 Oct - - - - - Nov 1 - - 2 3 Dec 35 - 1 - 36 Jan – 2022 2 1 - - 3 Feb 4 - - - 4 March 12 - - - 12 Total 225 3 9 7 244
  • 21. Total No. of Water samples = 225 Rs: 189380 Total No. of Soil samples = 3 Rs: 2100 Total No. of Animals samples = 7 Rs: 4220 Total No. of Feed Samples = 9 Rs: 4760 Total No. of Samples = 244 Revenue: Rs. 2,00,460/- April 2021 to March 2022
  • 22. April 2022 to February 2023 Month Water Samples Soil Samples Feed Samples Animals (shrimp & Fish) No. of Samples April – 2022 111 - - - 111 May 07 - - - 07 June 26 - - 01 27 July 27 7 - - 34 Aug - - - - - Sep 25 - - - 25 Oct 40 30 - - 70 Nov - - - - - Dec - - - - - January - 2023 02 - - - 02 Feb 02 05 - - 07 TOTAL 283
  • 23. April 2022 to February 2023 Total No. of Water samples = 240 Rs: 147775 Total No. of Soil samples = 42 Rs: 9700 Total No. of Animals samples = 1 Rs: 650 Total No. of samples = 283 Revenue: Rs: 169105
  • 24. TANII – 2017-2020 • E-Fish health surveillance and monitoring network to improve fisheries production in Tamil Nadu. (Sc.No.XV-102-04) Total Budget : Rs. 59.4 lakhs Duration : Three years Date of start : 23.03.2017 Date of closure (tentative, if it is ongoing) : 30.09.2020
  • 25. Expenditure 2021-2022 • Expenditure details on TNJFU Referral lab for the year of 2021-2022 Recurring expenses : Rs. 83,768/- • Manpower expenses: 2,86,500/- 2022-2023 • Expenditure details on TNJFU Referral lab for the year of 2022-2023 (February) • Recurring expenses : Rs. 5,500/- • Manpower expenses: 2,85,000/-
  • 26. Cost Benefit Analysis 2021-2022 • Total Expenditure : 3,70,268 /- • Total Revenue : 2,00,460/- • Cost Benefit Ratio: 0.54 2022-2023 • Total Expenditure : 2,90,500 /- • Total Revenue : 1,58,125/- • Cost Benefit Ratio: 0.54
  • 27. Future Plan: • To give more publicity to fish and shrimp farmers for sample analysis. • On spot sample analysis will be carried out. • Online UPS 10 KVA to be purchased for routine sample analysis. • Repairing of mobile van to be carried out. • To apply for NABL Accreditation.