1. IDEXX Influenza A Ab Test IDEXX Influenza A Ab Test
Test With Confidence™
Equine Specificity and Sensitivity
A panel of 73 sera was generously provided by
the Irish Equine Centre. Twenty sera originating
Numbers and percentages of positive equine sera in the
IDEXX Influenza A Ab Test
Any subtype. Multiple species.
from seronegative ponies (SRH*, n = 10) and
seronegative weanlings (SRH, n = 10) were all
Category Total (n) Positive (n) Sensitivity One highly accurate test.
negative on the IDEXX Influenza A Ab Test, resulting Vaccinated race horses 20 19 95%
in a specificity of 100%. Other categories of sera Influenza A is a zoonosis that poses a threat How the test works: Because the IDEXX Influenza A Ab Test is a blocking
High antibodies against type 2; seronegative for type 1 10 9 90%
and results in the IDEXX Influenza A Ab Test are to human and animal health worldwide. ELISA format, when influenza A antibodies are in the serum sample, they
specified in the table to the right. Numbers of 14 days postchallenge with a European strain
of type 2 influenza
7 6 86% Accurately monitoring and detecting exposure prevent the conjugate from binding with the nucleoprotein and the sample has
sera per category are rather small, just allowing is critical in preventing its spread. Yet numerous minimal color. Color development is inversely proportional to the amount of
an indication of relative sensitivities as compared Vaccinated horses postchallenge with European strain
10 10 100% variables make consistent detection a challenge— antibody in the sample.
of type 2 influenza
with HI and SRH data. For vaccinated or recently markedly different levels of pathogenicity, varied
challenged horses with high antibody levels in SRH/ Seronegative horses postvaccination, vaccine contained type
6 5 83% symptoms, numerous subtypes and cross-species
1 and type 2 influenza Test protocol
HI tests, relative sensitivities varied at 83–100%. antigenic differences. The IDEXX Influenza A
Samples: 1:10 dilution (15 µL sample into 135 µL sample diluent)
*SRH = Single radial hemolysis Ab Test overcomes this challenge, detecting Prepare dilutions Controls: Prediluted
antibodies to any influenza A subtype across avian,
Incubate 60 minutes (±5 minutes) at room temperature
swine, equine, canine, feline and zoo animals.
Rapid. Dependable. Efficient.
Efficient testing and exceptionally accurate results Wash 3–5 times with wash solution
leads to more confident decisions.
Add conjugate; incubate Anti-AI HRPO; 30 minutes (±2 minutes) at room temperature
Highly accurate results are just one of the benefits. • etects antibodies within 8–13 days for avian species, comparable to AGID,
D
Your lab operates more smoothly and you can and 18–25 days for other species The Common Element in Influenza A Detection Wash 3–5 times with wash solution
screen more confidently because this test: • ses a familiar protocol and ready-to-use reagents that minimize the
U When it comes to influenza A screening, knowing Add substrate; incubate TMB; 15 minutes (±1 minutes) at room temperature
• eplaces multiple sub type-specific tests with a
R learning curve and are suitable for semiautomatic or robotic processing what to look for is key. Symptoms vary; subtypes
Stop, read Read plate at A(650)
single blocking ELISA and antigens differ. The IDEXX Influenza A Ab
• mproves throughput by letting you test more than one species at a time
I
Test provides consistent detection across species
• ses the same conjugate, regardless of
U • educes the need for confirmatory tests with its 99.7% specificity
R Results: Results are expressed as S/N values (sample to negative
because it is based on a constant amongst so
underlying species, so you have less antigen control ratio).
much variation—a highly conserved epitope of the
and reagent inventory and variation
influenza A nucleoprotein. It can detect exposure in
Interpretation: Avian test cutoff: S/N ≥0.5 is negative; S/N 0.5 is positive.
any species that generates an antibody response
Other species test cutoff: S/N ≥0.6 is negative; S/N 0.6 is positive.
to the nucleoprotein, regardless of influenza
For more information about the IDEXX Influenza A Ab Test, please subtype or antigenic variance between species. With balance comes confidence
contact your IDEXX representative or visit idexx.com/influenza. With its 99.7% specificity and 95.4% sensitivity, the IDEXX Influenza A Ab
Test provides near perfect balance, accurately detecting exposure with few
false positives and no subtype limitations. So you get more comprehensive
Corporate Headquarters European Headquarters Asian Headquarters screening, fewer confirmatory tests and more confidence in your results.
IDEXX Laboratories, Inc. IDEXX Europe B.V. IDEXX Laboratories, Inc.
One IDEXX Drive Scorpius 60 Building F 3F-5 No. 88, Rei Hu Street Studies consistently demonstrate the efficacy of the IDEXX Influenza A Ab Test
Westbrook, Maine 04092 USA 2132 LR Hoofddorp Nei Hu District at detecting exposure to influenza A in avian, feline, canine, equine, swine and
The Netherlands 11494 Taipei, Taiwan
Tel: 1-207-556-4890 or zoo animals, with performance levels comparable to those of homologous
1-800-548-9997 Tel: 31-23-558-70-00 or Tel: +886 2 6603 9728
hemagglutination inhibition (HI).
Fax: 1-207-556-4826 or 00800-727-43399 Fax: + 886 2 2658 8242
1-800-328-5461 Fax: 31-23-558-72-33
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IDEXX and Test With Confidence are trademarks or registered trademarks of IDEXX Laboratories, Inc. or its affiliates in the
United States and/or other countries. The IDEXX Privacy Policy is available at idexx.com.

2. IDEXX Influenza A Ab Test
Avian Influenza (AI) Avian specificity and sensitivity Contact and Direct Exposure to Swine Influenza Swine Overall Test Performance for Swine Influenza
AI-inoculated chicken population (n = 12) AI Serological Status* Homologous HI Homologous HI Contact/direct field exposure
H1N1 H5N2 H7N3
and negative population:
+ – Study 461 + – + –
AGID positive
1.20 Specificity = 99.6%
+ 1192 15 + 56 0 + 242 1 (95% CI, 97.7%–100%)
1.00 IDEXX Influenza A IDEXX Influenza A IDEXX Influenza A
Sensitivity = 95.3%
Ab Test Ab Test Ab Test
– (95% CI, 91.8%–97.3%)
0.80 – 58 4992 – 0 0 12 276
S/N (mean)
Kappa = 0.95
0.60
Negative Specificity = 99.7%
Positive Test cutoff (0.50) Sensitivity = 95.4%
0.40
*Determined by consensus analysis of exposure to
AI virus and reference test results. Vaccinal Exposure to Swine Influenza Field Cases of Swine Influenza
0.20
0
0 5 8 13 21 28 Homologous HI Homologous HI
Days Post Inoculation Study 456 + – Case A + –
Canine Influenza (H3N8) Canine and feline data summary + 3 0 + 74 0
IDEXX Influenza A IDEXX Influenza A
Ab Test Ab Test
Antibody response on IDEXX Influenza A Ab Test Number of positives from total tested – 6 19 – 0 0
Day 18 Day 25 Day 32
1.20
Study 480 + – Case B + –
Canine 3/8 7/8 8/8
1.00
Feline 6/10 8/10 10/10 + 42 2 + 8 0
IDEXX Influenza A IDEXX Influenza A
0.80
Ab Test Ab Test
Negative – 5 7 – 2 0
S/N
0.60
Positive Test cutoff (0.60)
0.40
0.20
Swine Influenza Negative Serum Samples
0
0 7 11 14 18 25 32 39 46
Days Post Inoculation Results: IDEXX Influenza A Ab Test had 99.6% specificity
Feline Influenza (H3N8) Mean = 0.94 ± 0.10
70 3.4 sd’s from test cutoff
Specificity = 99.6%
Antibody response on IDEXX Influenza A Ab Test 60
50
Frequency
1.20 40
30
1.00
20
0.80
10
Negative
S/N
0.60
Positive Test cutoff (0.60) 0
0 0.1 0.2 0.3 0.4 0.5 0.6 0.7 0.8 0.9 1.0 1.1 1.2
0.40
S/N
0.20
0
0 7 11 14 18 25 32 39 46
Days Post Inoculation