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BATERIAS PRUEBA PRACTICA
1.- COCOS GRAM +
CATALASA +
(Sthapylococcus)
CATALASA –
(Streptoccoccus y Enterococcus)
1) O/F
2) Urea
3) Azúcares:
• Sacarosa
• Xilosa
• Trehalosa
• Manosa
• Manita
• Maltosa
4) Plasma (coagulasa)
5) Suero ( para antibiograma
6) Agar Peptona
7) Agar Chapman
8) Agar AB (Muller –Hiltonn)
TOTAL = 11 tubos + 3 placas
1) Tolerancia a NaCl 6,5%
2) Bilis esculina
3) Hìpurato de sodio
0,8 ml  cultivo
0,2 ml  Tricloruro ferrico para hipurato
4) Agar sangre para antibiograma directo
BACITRACINA
OPTOQUINA
TOTAL = 3 tubos + 1 placa
2.- BACILOS GRAM NEG
OXIDASA –
( Enterobacteria)
OXIDASA +
1) O/F
2) Kligler
3) Fenilalanina
4) Caldo comun (indol)
5) Aminoácidos:
• Control
• Ornitina
• Lisina
• Arginina
6) Urea
7) Citrato
8) Nitrato
9) Arabinosa
10) Sorbitol
11) Malonato
12) Placa Mc Conkey
TOTAL = 15 tubos + 1 placa
1) O/F
2) Kligler
3) Fenilalanina
4) Caldo común (motilidad - indol)
5) SALES
• SIN NaCl
• 6,5% NaCl
• 8% NaCl
6) Aminoácidos:
• Control
• Ornitina
• Arginina
7) Urea
8) Arabinosa
9) Nitrato
10) Citrato
11) Manitol
12) Maltosa
13) Xilosa
14) Sacarosa
15) Placa agar Sangre (desarrollo 42 º C)
16) Placa Mc Conkey
17) Placa Gelatina
TOTAL = 19 tubos + 3 placas
Lo que se hace con Acinetobacter que puede ser bacilo o coco gram negativo, es que se le
hace oxidasa esta dara negativa, se le hara la misma bateria que a una enterobacteria,
como ambos son oxidasa negativa no la podemos confundir con neisseria o moraxella, ya
que estas son oxidasa +.
3. – COCOS GRAM NEG (diplococos )
Catalasa +
Oxidasa +
1) CTA
• Glucosa
• Fructosa
• Sacarosa
• Manita
• Lactosa
• Maltosa
2) Nitrato
3) PLACA DNAasa (NEISSERIA - / moraxella + )
4) Placa agar Peptona (MICOAEROFILIA)
TOTAL = 7 TUBOS + 2 PLACAS
4.- BACILOS GRAM POSITIVOS
Catalasa +
1) CTA
• Glucosa (O/F)
• Maltosa
• Sacarosa
• Ramnosa
• Galactosa
• Fructosa
• Xilosa
2) Esculina
3) Nitrato x2 (37º Y 25º C)
4) Urea
TOTAL = 12 TUBOS (CTA)

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Baterias prueba practica (2)

  • 1. BATERIAS PRUEBA PRACTICA 1.- COCOS GRAM + CATALASA + (Sthapylococcus) CATALASA – (Streptoccoccus y Enterococcus) 1) O/F 2) Urea 3) Azúcares: • Sacarosa • Xilosa • Trehalosa • Manosa • Manita • Maltosa 4) Plasma (coagulasa) 5) Suero ( para antibiograma 6) Agar Peptona 7) Agar Chapman 8) Agar AB (Muller –Hiltonn) TOTAL = 11 tubos + 3 placas 1) Tolerancia a NaCl 6,5% 2) Bilis esculina 3) Hìpurato de sodio 0,8 ml  cultivo 0,2 ml  Tricloruro ferrico para hipurato 4) Agar sangre para antibiograma directo BACITRACINA OPTOQUINA TOTAL = 3 tubos + 1 placa
  • 2. 2.- BACILOS GRAM NEG OXIDASA – ( Enterobacteria) OXIDASA + 1) O/F 2) Kligler 3) Fenilalanina 4) Caldo comun (indol) 5) Aminoácidos: • Control • Ornitina • Lisina • Arginina 6) Urea 7) Citrato 8) Nitrato 9) Arabinosa 10) Sorbitol 11) Malonato 12) Placa Mc Conkey TOTAL = 15 tubos + 1 placa 1) O/F 2) Kligler 3) Fenilalanina 4) Caldo común (motilidad - indol) 5) SALES • SIN NaCl • 6,5% NaCl • 8% NaCl 6) Aminoácidos: • Control • Ornitina • Arginina 7) Urea 8) Arabinosa 9) Nitrato 10) Citrato 11) Manitol 12) Maltosa 13) Xilosa 14) Sacarosa 15) Placa agar Sangre (desarrollo 42 º C) 16) Placa Mc Conkey 17) Placa Gelatina TOTAL = 19 tubos + 3 placas Lo que se hace con Acinetobacter que puede ser bacilo o coco gram negativo, es que se le hace oxidasa esta dara negativa, se le hara la misma bateria que a una enterobacteria, como ambos son oxidasa negativa no la podemos confundir con neisseria o moraxella, ya que estas son oxidasa +.
  • 3. 3. – COCOS GRAM NEG (diplococos ) Catalasa + Oxidasa + 1) CTA • Glucosa • Fructosa • Sacarosa • Manita • Lactosa • Maltosa 2) Nitrato 3) PLACA DNAasa (NEISSERIA - / moraxella + ) 4) Placa agar Peptona (MICOAEROFILIA) TOTAL = 7 TUBOS + 2 PLACAS 4.- BACILOS GRAM POSITIVOS Catalasa + 1) CTA • Glucosa (O/F) • Maltosa • Sacarosa • Ramnosa • Galactosa • Fructosa • Xilosa 2) Esculina 3) Nitrato x2 (37º Y 25º C) 4) Urea TOTAL = 12 TUBOS (CTA)