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THE EFFECT OF DIET ON DMSP ACCUMULATION AND TASTE IN SOUTH AFRICAN ABALONE ( Haliotis midae )   Deborah Robertson-Andersson 1 ; A. J. Smit 2 ; S. Peall 3  and   Bolton J. J 1   1 Botany Department, U CT 2 School of Biological and Conservation Sciences, UKZN 3 Hearshaw and Kinnes Analytical Laboratory, UCT,
What do DMSP and abalone taste have to do with each other? ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Integrated abalone-macroalgal cultivation ,[object Object],[object Object],[object Object],[object Object],(Naidoo  et al ., in press)
Benefits of a mixed algal diet KELP ONLY DIET MIXED ALGAL DIET
However….. ,[object Object]
Abalone  Mariculture Operations in South Africa Abalone  Mariculture Operations in South Africa Durban Port Elizabeth Cape Town I&J Abalone  Atlantic Fishing Sea Plant Products Roman Bay Marine Growers HIK Farm Abagold Aquafarm Development Avuca Abalone Fish Bay Mariculture Jacobsbaai Sea Products West Coast Abalone Port Nolloth Sea Farms Wild Coast Abalone Abaseed
Questions… ,[object Object],[object Object]
Preliminary investigations  ,[object Object],[object Object],[object Object],16.8 15.7 18.5 Methionine   (mg.g -1  protein) 28.1 Gracilaria 13.3 Ulva 29.1 Ecklonia Cysteine  (mg.g -1  protein)
Preliminary investigations  ,[object Object],[object Object],16.8 15.7 18.5 Methionine   (mg.g -1  protein) 1.6 - 48.6 28.1 Gracilaria 4101 - 7961 13.3 Ulva 0.5 - 46.1 29.1 Ecklonia DMSP  ( μg.g -1  fresh mass ) Cysteine  (mg.g -1  protein)
Background: DMSP and DMS in algae ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
 
DMSP and DMS ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Do abalone contain DMSP?
If they contain DMSP, where is it accumulated? Top of  foot Epipodial frill
[object Object],Preliminary investigations  ,[object Object],[object Object],TF F BF AM Top of foot  Epipodial frill Bottom of foot Abductor muscle
Abductor muscle preparation Step 1 Step 4 Step 3 Step 2
DMSP diet experiment ,[object Object],[object Object],[object Object]
DMS content in canned abalone ,[object Object],[object Object]
 
Depuration  experiment ,[object Object]
Wild abalone ,[object Object],[object Object],[object Object],> 1 000 g 250 – 750 g 100 -200 g 10 -20 g Small Medium Large Smallest
 
 
Continuing studies… ,[object Object],[object Object],[object Object]
The Future ,[object Object],[object Object],[object Object]
  THANK YOU ACKNOWLEDGEMENTS I would like to extend special thanks to the following people and organizations without whose help this project would be impossible: Swedish and South African Collaborative Program I & J Mariculture farm JSP Mariculture farm Wild Coast Abalone  Abagold N R F J SP
Preliminary investigations  ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
DMSP and DMS  ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
DMSP and DMS in herbivores ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
The Future ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

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THE EFFECT OF DIET ON DMSP ACCUMULATION AND TASTE IN SOUTH AFRICAN ABALONE (Haliotis midae)

  • 1. THE EFFECT OF DIET ON DMSP ACCUMULATION AND TASTE IN SOUTH AFRICAN ABALONE ( Haliotis midae ) Deborah Robertson-Andersson 1 ; A. J. Smit 2 ; S. Peall 3 and Bolton J. J 1 1 Botany Department, U CT 2 School of Biological and Conservation Sciences, UKZN 3 Hearshaw and Kinnes Analytical Laboratory, UCT,
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  • 4. Benefits of a mixed algal diet KELP ONLY DIET MIXED ALGAL DIET
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  • 6. Abalone Mariculture Operations in South Africa Abalone Mariculture Operations in South Africa Durban Port Elizabeth Cape Town I&J Abalone Atlantic Fishing Sea Plant Products Roman Bay Marine Growers HIK Farm Abagold Aquafarm Development Avuca Abalone Fish Bay Mariculture Jacobsbaai Sea Products West Coast Abalone Port Nolloth Sea Farms Wild Coast Abalone Abaseed
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  • 13. If they contain DMSP, where is it accumulated? Top of foot Epipodial frill
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  • 15. Abductor muscle preparation Step 1 Step 4 Step 3 Step 2
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  • 25.   THANK YOU ACKNOWLEDGEMENTS I would like to extend special thanks to the following people and organizations without whose help this project would be impossible: Swedish and South African Collaborative Program I & J Mariculture farm JSP Mariculture farm Wild Coast Abalone Abagold N R F J SP
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