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New sequencing Storage Guy Coates Wellcome Trust Sanger Institute [email_address]
About the Institute ,[object Object]
~700 employees. ,[object Object],[object Object]
We have active cancer, malaria, pathogen and genomic variation studies. ,[object Object],[object Object]
Recent initiatives: 1 1000 Genomes
Overview ,[object Object]
2. The data explosion
3. Building flexible systems
4. Future directions
Scramble for Next-gen sequencing
Classic Sanger “Stealth project” ,[object Object]
Not long after: ,[object Object],[object Object]
What are we dealing with? ,[object Object]
“There are known knowns.” ,[object Object]
How much data will be coming off? ,[object Object],[object Object]
Will we be CPU bound or IO bound?
What will the growth rate be? ,[object Object]
Modular ,[object Object]
Easy to scale.
Small unit of failure; system failure should only affect one sequencer.
Hard to right-size, especially if things change. CPU Disk Seq CPU Disk Seq CPU Disk Seq
Monolithic ,[object Object]
Complicated. Clustered storage at scale is hard.
Eliminating SPOF is hard.
Scale out can be expensive. CPU FARM Cluster filesystem Seq Seq Seq Network Storage fabric
Supersize me ,[object Object]
384 cores for the sequencers, the rest for other analysis.
300TB of storage. ,[object Object]
Lots of performance if we need it.
Plenty of space to cope with changing needs. ,[object Object],[object Object],[object Object]
Compute farm analysis/QC pipeline suckers Data pull ... Final Repository (Oracle) 100TB / yr staging area 300 TB Lustre EVA Seq 1 Seq 38
Hurrah! ,[object Object]
The Data Explosion
The scary graph Instrument upgrades Peak Yearly capillary sequencing
Sequencing is not everything... ,[object Object]
Expanding Everything ,[object Object]

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Storage for next-generation sequencing