1. Donors and Chagas Disease 11 th Congress of the International Society for Organ Donation and Procurement Buenos Aires – Argentina 27-30 Nov 2011

2. Disclosures No conflict of interest Roberta Lattes [email_address] Instituto de Nefrología/Nephrology Buenos Aires – Argentina

3. Chagas disease

6. - PCR PCR

9. 26 labs in 16 countries 70 samples checked Inter-lab evaluation Intra-lab evaluation PCR kDNA (330 bp) Highly repetitive sequences: Detection of low parasitic loads Useful for chronic infection diagnosis Early detection of reactivation and transmission PCR satellite (180 bp ) Nuclear DNA 50.000-100.000 p/parasite Kinetoplast DNA 40.000-80.000 p/parasite 2011;5(1):e931

11. Chagas infected donors Effective infected donors in Argentina Source: INCUCAI 2011 Not all Tx teams accept infected donors 3.7 5.1 4.6 4.6 2.5 5.7

12. Kidney transplant: outcome Total Kidney Tx R-/D+ (n=56) 2.6% Infection Transmission (day +35 to 165 ) 12.5% Diagnosis Hospital Argerich 650 27 4 Strout 1 Strout + fever 2 Strout+ Paniculitis 1 INBA 1019 27 2 Strout 1 PCR+Strout 1 Fundación Favaloro 478 2 1 PCR