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Cassava Study:

One recent study concluded that by 2050, biomass theoretically could supply 65% of the world’s
current energy consumption, with sub-Saharan Africa, the Caribbean, and Latin America accounting
for roughly half of this global potential. In tropical countries, high crop yields and lower costs for land
and labor provide an economic advantage that is hard for countries in temperate regions to match.

Cassava has erupted into the first decade of the third millennium as a crop that can contribute to agro-
industrial and small-farmer development in the tropics. Cassava is progressively shifting roles from
being a staple food for human consumption to becoming an efficient industrial crop particularly in
developing economies of Asia, Latin America, and Africa. Cassava owes part of its popularity to its high
rate of conversion of solar energy into starch per unit area, as compared to other starchy staple crops
e.g., rice and maize. In addition to the high carbohydrate content (74 - 85% of its total storage root dry
weight), it contains varying amounts of vitamin C (ascorbic acid), vitamin A (carotenes), iron, zinc,
calcium, potassium and protein that account for its wide popularity .

To a large extent, the difference between the progression of cassava starch-based industries in the ACP
region and Asia is the differential Government commitment towards cassava commercialization and
increasing investment in research and development. The livestock sector has also been targeted for
utilisation of cassava either through minimal processing i.e., silage or use of well-refined feed rations.

Equally important is the need to undertake strategic research to identify, develop and promote cassava
varieties that can meet animal feed standards and satisfy the needs of small herders as well as large
livestock producers for high quality feed at affordable prices. Together, these initiatives will increase
the utilisation of cassava in animal feed and hence increase the profitability of the livestock sector in
the ACP region.

In Africa, most cassava research is geared towards addressing key production and post-harvest
constraints, with limited efforts devoted towards increasing the competitiveness of cassava in the
industrial sector. Nevertheless, some strategic pioneering research is underway in different parts of the
continent. For example, in partnership with the Swedish University of Agricultural Science, Uganda has
embarked on starch metabolism studies Also, the International Institute of Tropical Agriculture (IITA),
based in Nigeria, is having special interest in:

    1. enhancing beta carotene, zinc (Zn), iron (Fe) and protein levels in cassava;
    2. genetic modification of cassava starch functionality and;

    3. Development of cassava varieties with varied starch content and profiles.

It is rather surprising to note that despite the huge acreage of cassava in Africa, Government
commitment to this crop varies considerably between countries. For example, Ghana and Nigeria stand
out as countries on the continent that have witnessed full and genuine presidential support for
promotion of cassava starch.

The above selected examples strongly illustrate existing opportunities that can permit the
transformation of cassava from being a staple food crop to an industrial crop in the ACP region, as has
been witnessed in Asia and Latin America. These opportunities are broadly characterized as:

    1. cassava being irreplaceable by other starches i.e., cassava starch as the best;
    2. cassava is rated poor as compared to other starch crops and;

    3. Cassava is easily interchangeable with other starches.
Cassava starch utility:

Food products- Native tapioca starch is widely applied in food recipes such as bakery products. It is
also used to produce extruded snacks and tapioca pearls. Modified starch, or starch derivatives, has
been applied as thickening, binding, texturizing and stabilizing agents.Uses as fillers, sweeteners,
flavor carriers and fat replacement in many food products include canned food, frozen food, dry mixes,
baked goods, snacks, dressings, soups, sauces, dairy products, meat and fish products and infant food.

Beverage- Modified tapioca starch is used as a colloid stabilizer in beverages that include solid
constituents. Tapioca starch-based sweeteners can be produced with considerably higher yields than
sugar and are used in beverages as a sugar replacement. In combination with other sweetener
components, it can usually contribute to satisfying the customer’s requirement. High dextrose
equivalent syrups of tapioca-based hydrolysate are also good sources of easily fermentable sugars for
brewery applications.

Confectionery- Native tapioca and diverse types of modified tapioca starch are used in confectionery
for different purposes such as gelling, thickening; texture stabilizing, foam strengthening,
crystallization inhibition, adhesion, film forming, and glazing. Low viscosity tapioca starches are widely
used in gelled confectioneries such as jellies and gums. The most often used one is acid-thinned starch
due to its high retro-gradation and gel formation characteristics, which are enhanced by the presence
of sugars. Powdered starches are used as mould release agents when casting confectioneries. Starch-
based polyols make the manufacture of sugar-free chewing gum possible.

Chemicals- Tapioca starch-based syrups are obtained economically by acid and/or enzyme processes
and used as feedstock to make various chemicals, including monosodium glutamate, amino acids,
organic acids, alcohols, ketones, vitamins and antibiotics.
Production techniques include chemical reaction, fermentation and other biotechnological processes.

Adhesives & Glue- Tapioca starch-based dextrins are excellent adhesives and used in many
applications including corrugated board, paper-bags, laminated board, gummed paper, tapes, labels,
stamps and envelopes.

Paper- Modified tapioca starches are applied in the paper industry to improve paper quality, increase
production rates, and improve pulp yield. Cationic starches are employed to flocculate pulp, increasing
de-watering rates on the wet end. Faster machine speeds and better pulp yields result. The starch
remains in the finished paper, acting as an internal sizing agent to increase the paper strength. Low
viscosity starches, such as oxidized starches, are applied as surface sizing to improve the strength and
control ink absorption properties for printing and writing. Modified tapioca starches are also used as
 pigment binder for surface coating to obtain a smooth, white paper.

Textile- Tapioca starches are used in the textile industry as sizing agents to stiffen and protect the
thread for improved weaving efficiency. They are also used as finishing agents to obtain smooth
fabrics, and color thickeners to obtain sharp and durable printed fabrics. For this purpose, thin-boiled
starches are usually preferred.

Pharmacy and Cosmetics- Native and modified tapioca starches are used as binders, fillers and
disintegrating agents for tablet production. Specialty modified starches are used as a carrier for skin
moisturizers, which are frequently mineral oil based. Other modified starches are used as emulsifiers,
encapsulating agents (vitamins), sizing (mousse for hair), thickeners (shampoo), etc.

Biodegradable materials- Native and modified tapioca starches can be blended with petroleum-based
or synthetic polymers to improve the biodegradability and minimize the production cost of more
environmentally friendly materials.
Applications of modified tapioca starch in food industry-
Starch type Functionality/Property Application- Pre-gelatinized starch Thickening, Cold water Instant
soups, instant soluble puddings, sauces, bakery mixes, frozen food Acid-thinned starch Lower viscosity,
High Gum, candies, formulated retrogradation, Strong gel liquid food Dextrins Binding, coating,
Confectionery, baking,encapsulation flavorings, spices, oils Oxidized starch Stabilizer, adhesives,
Formulated food, batter,gelling, clarifying agent gum, confectionery Starch ethers Stabilizer, fat
replacement Soups, puddings, frozen(Hydroxy-alkyl starch food Carboxy-methyl starch)Starch ester
Stabilizer, thickening Candies, emulsion (Acetylated starch agent, clarification Phosphate mono-ester
starch)Cross-linked starch Thickening, stabilizer, Pie fillings, breads, frozen (Di-starch phosphate)
texturizing agent products, bakery, pudding,instant foods, soups,gravies, salad dressing.

More than 228 million tons of cassava was produced worldwide in 2007, of which Africa accounted
for 52%. In 2007, Nigeria produced 46 million tons making it the world's largest producer. According to
2002 FAO estimates, Africa exports only one ton of cassava annually. Cassava production depends
on a supply of quality stem cuttings. The multiplication rate of planting materials is very low
compared to grain crops, which are propagated by true seeds. In addition, cassava stem cuttings are
bulky and highly perishable as they dry up within a few days.

Harvesting - Nineteen million hectares of cassava were planted worldwide in 2007, with about 63% in
Africa. Cassava requires less labor than all other staple crops (21% in working days as compared to
maize, yam and rice). However, it requires considerable postharvest labor because the roots are
highly perishable and must be processed into a storable form soon after harvest. Roots can be
harvested between six months and three years after planting.

Consumption- Nearly every person in Africa eats around 80 kilograms of cassava per year. It is
estimated that 37% of dietary energy comes from cassava. The Democratic Republic of Congo is the
largest consumer of cassava in SSA, followed by Nigeria.

Disease and constraints- Major pests of cassava in SSA are the cassava green mite and the variegated
grasshopper. The main diseases affecting cassava are cassava mosaic disease (CMD), cassava bacterial
blight, cassava anthracnose disease, and root rot. CMD alone accounted for an estimated 47% of East
and Central Africa's cassava production losses during a serious outbreak beginning in the early 1990's
until 2006. Pests, disease and poor cultivation practices combined can cause yield losses as high as 50%
in all of Africa.

Opportunity in Biofuels development:

Biofuels offer Africa the chance to supply itself with alternative energy sources, and also to become a
major supplier of these sources for developed markets. Yet, challenges - from creating the relevant
infrastructure to competition for biofuels crops from food markets - remain. It is rather surprising to
note that despite the huge acreage of cassava in Africa, Government commitment to this crop varies
considerably between countries. For example, Ghana and Nigeria standout as countries on the
continent that have witnessed full and genuine presidential support for promotion of cassava starch.
The above selected examples strongly illustrate existing opportunities that can permit the
transformation of cassava from being a staple food crop to an industrial crop in the ACP region, as has
been witnessed in Asia and Latin America.

These opportunities are broadly characterized as:

    1. cassava being irreplaceable by other starches i.e., cassava starch as the best;
    2. cassava is rated poor as compared to other starch crops and;

    3. Cassava is easily interchangeable with other starches.
Utilizing biomass to produce biofuels and chemical products is an important direction for realizing
sustainable development. Biomass is a kind of renewable resource, but it is also significant to promote
an ecological development pattern in Biorefinery processes by raising resource efficiency, reducing
consumption of energy and water, decreasing waste emission as well as constituting industrial
symbiosis products network. Thus, it is possible to reach overall sustainable development of the
Biorefinery industry.

Different biomass raw materials such as corn, wheat, sweet potato, cassava and oil plants are
fermented to produce different kinds of products including fuel ethanol, biodiesel, 1,3-propanediol,
butanediol, acetone, butanol, lactic acid and dry barm. And bioethanol will be converted into
bioethene in the next stage.

The key to cassava's future in global and domestic starch markets will be improvements in efficiency
and quality, and a reduction in production costs. For a model of successful cassava starch industry
development, African and Latin American countries need look no further than Thailand, the world's No.
1 producer. The Thai industry began more than 50 years ago, and expanded rapidly during the 1990s,
when trade restrictions sharply reduced the European market for Thai dried cassava chips, used as
animal feed.

Thailand now uses about 50 percent of its annual cassava root production, of around 18 million tons, to
extract some two million tons of starch. Half of it is destined for domestic food and non-food
industries, the rest being exported, mainly to Japan and Taiwan, and increasingly in the form of
higher-value modified starch for specialized applications. Thailand is the world’s largest exporter of
tapioca starch and starch derivatives, with annual production of over 2 million tons of starch. The
country is also exploring a promising new market for its starch - as raw material for production of
ethanol used as a biofuels. The leading Thai petroleum company has announced a feasibility study for a
plant that would use cassava to produce one million litres of ethanol per day.

Fledgling cassava starch industries concentrate initially on meeting domestic demand - a study of
global cassava markets found that tropical countries were importing annually maize starch and
derivatives to a value of more than $80 million. In many countries, the study found, almost all imports
could be replaced with locally-made cassava starch or, for simple applications, even by good-quality
cassava flour.

In Africa, there are signs of growing interest in using locally-made cassava starch as an import
substitute. Cassava starch start-ups have recently been established in Uganda, Tanzania, and
Madagascar, while in Malawi industries have shown interest in buying local cassava starch for use in
paper, cardboard, sweets and food processing. Meanwhile, the region's leading cassava producer,
Nigeria, has recently announced an ambitious programme aimed at producing ethanol biofuel from
cassava. With better farmer management, we can produce an average of 30 to 50 tonnes of cassava per
hectare as opposed to the 5 tons per hectare from local varieties commonly used by farmers in these
areas.

In addition, opportunities exist for African-produced biofuels to enter the U.S. market under the
African Growth and Opportunities Act, Washington’s answer to EBA. Import markets are developing in
other major global economies like Japan, and a handful of African countries are looking at biofuel use
as well. The strategies established in the main four countries are also laying down guidelines for tax
breaks and at times guaranteeing prices for producers and establishing regulatory structures.

Many of Africa’s most viable biofuel producers - in terms of production costs for sugarcane, maize or
cassava - are landlocked, however, and don’t have the transportation infrastructure in place to enable
export of the fuels, or immediate plans for infrastructure investment like Mozambique. Several
countries in Africa are developing biofuel policies to help state-owned and commercial companies alike
bring about biofuel production, and some are creating local markets. Small countries like Rwanda,
which are not only landlocked but suffer from high petroleum import costs, are looking at biodiesel
production for the local market.

Although production of biofuels in West Africa may at present be insignificant compared to the rest of
the world market, the countries in the region seem determined to exploit what they see as a godsend
of a market.

A recent report by the OECD shows that the West African nations are viewing this new market as a
promising way of boosting their own standing and enhancing their economies. In almost all West
African countries, the new biofuels business is being negotiated by and carried out by large multi
nationals.
These countries are seen as rich resources to be exploited, because of their potential to grow biofuel
crops. They have vast swathes of unused agricultural land that has high potential to grow the raw
materials for biofuels. The OECD says that this potential for exploitation by the multinationals and the
potential for growth in the biofuels industry in West Africa present several challenges to the countries.
It says they have to ensure that land competition with land used for food purposes is avoided and that
natural capital is observed.

The OECD says they must "negotiate 'win-win' agreements with multinationals, involving three parties:
the public and private sectors as well as representatives from local communities. This would allow the
best possible benefit to be obtained from the economic and social effects of producing green fuels: to
ensure that sufficient income is earned, the largest possible number of local jobs is created and that
the development of cooperative distilleries and/or local investment is encouraged."

The OECD adds that those investing in biofuel production in these countries will naturally gravitate
towards the countries where conditions are most favorable - where regulatory, environmental and
ethical constraints are at a minimum. Already four countries in the region - Ghana, Nigeria, Sengal and
Mali - have established national strategies for biofuels production. The national strategies have set
production targets for the four countries which will see 320,000 hectares of Jatropha curcas in Senegal
in 2012, a million hectares in Ghana in the medium term, 25 million litres of ethanol annually in Mali
between 2008 and 2023.

    •   Mali has also just set up a biofuels agency to centralise the government policies and set
        technical and quality standards for biofuel products. The National Biofuel Development Agency
        (ANADEB) will be responsible for overseeing the National Energy Policy and the Renewable
        Energy Development Strategy brought in in 2006, and last year's National Biofuel Development
        Strategy.

        However, the OECD concludes that there are genuine prospects for Africa and West Africa in
        particular to make the most of the situation, provided the opportunity of the new market is
        grasped together with the positive impacts it will have on economic growth, employment and
        development and at the same time while it recognizes the risks the new market will also offer.
    •

    •   The Zambian Government's vision for the agriculture sector up to 2015 and beyond will be
        promoted taking into account the comparative advantage in crops, livestock, and fisheries
        production. The long term vision for the sector encompasses achieving national food security
        for the majority of the Zambian population through increased yields and improved post harvest
        management and utilisation. The Government will Endeavour to develop commercial
        agriculture with all the farmers producing for domestic and export markets.

Promoting a competitive and efficient agriculture base on regional comparative advantage and the
development of a diversified agriculture linked to a well developed agro-business industry for value
adding and exports are other efforts being pursued. Notwithstanding the emphasis on development of
small scale famers, a conducive environment is being provided for growth of large scale farming sector
in order to maximize the synergies between the two. Focus will continue being on providing public
goods that are needed for efficient growth such as rural infrastructure, basic research, and disease
epidemic and pest control.

The role of the public sector will increasingly be confined to policy formulation, enforcement of
legislation, regulation, and inspection, maintenance of the national strategic food reserves.
Agribusiness is being encouraged and promoted to strengthen linkages with small scale farmers through
increased private sector participation in agriculture service delivery. The ministry of agriculture will
also ensure increasingly commercialization and cost share some services it is currently providing. The
Government will continue partnering with the private sector and Non Governmental Organisations and
cooperating partners to ensure attainment of food security for the majority household with at least 90
per cent of the population being food secured.

    •   The cassava processing industry in Indonesia is a very profitable business. Aside from the major
        starch product, the by-products can be used to manufacture other useful products. PT Budi
        Acid Jaya Tbk (BUDI) is one of the companies focusing on the production of cassava or tapioca
        starch in Indonesia. To take advantage of tapioca's by-product called 'onggok', the company
        uses it to produce higher value-added product such as citric acid.

With the country's focus on biofuels, the company has planned to build a cassava-based bioethanol
manufacturing plant with a capacity of 75,000 kilo liters. The plant costing US$43 million will be
located in Lampung, Indonesia. Deputy President Director of BUDI, Sudarmo Tasmin, said that the
development plan was a response to the promising prospect of renewable energy business and the
soaring oil price which now exceeds more than US$ 100 per barel. The establishment of the plant is
being conducted in collaboration with a Japanese company, to which 50% of the produced bioethanol
will be sold.
     • The project (IFAD-ICRISAT) facilitates entrepreneurs to utilize sweet sorghum stalks and
         cassava roots in producing ethanol, and seeds of jatropha in producing bio-diesel. The above
         program will be implemented by sensitizing farmers, research partners and other stakeholders
         in the production and supply chain about biofuel production.

This will enable them to work together and make use of the project's research outputs, such as,
improved target crop cultivars, production packages, seed systems, processing technologies (including
management of effluents and exploitation of by-products), and learn about innovative input and
market linkages developed for different agro-eco-regions in the target countries.

In addition, the project draws upon the strength of small-scale farmers' know-how in formulating and
implementing various activities. The overall purpose of the project is thus to facilitate small-scale
farmers and landless poor to take advantage of the market demand for their crops for bio-fuel
production and/or utilize the bio-fuels for local use (e.g. running motor pump), which in turn, will help
them improve their livelihoods and rehabilitate the degraded lands (wherever jatropha and local
species of bio-diesel plantations are taken up). The project also envisages facilitating the development
of farmer-friendly procedures to enable them to take advantage of the CDM, of the Kyoto protocol, to
improve their livelihoods. The project contributes to energy self-sufficiency of the target countries.

Cassava Developments:

The crop produces reasonably well under marginal conditions of climate and soil and is frequently
identified as a famine reserve due to its tolerance to drought and infertile soils, and its ability to
recover from disease and pest attacks. It can also produce competitively in non-marginal areas.
Cassava offers the advantage of a flexible harvesting date, allowing farmers to keep the roots in the
ground until needed. Remarkably every part of the plant can be exploited: in addition to the roots the
fresh foliage is also consumed in several regions of the world and the lignified stems are used as
planting material. Because of its unique characteristics cassava grows in the marginal environments
where poverty and malnourishment are also very prevalent.

During the 1993-95 periods, about 16.5 million hectares were grown with cassava worldwide, producing
164 million tons of roots. For the period 1993 to 2020 annual growth of cassava production was
estimated to range between 1.74 and 1.95% / year. The area of cassava under marginal environments
has been continuously increasing, particularly for regions with poorer soils and lengthy dry seasons.

Research -In the past an emphasis was given to breeding cassava with white roots. However,
considerable efforts were recently invested in measuring the variability for its carotenoids contents in
roots and leaves from thousands of clones, along with other relevant information. It was found that
high levels of carotenoids (more than 1 and 96 mg/100 g of fresh root or leaf tissue, respectively) could
be found in certain clones. High levels of carotenoids were associated with yellow coloration, which
facilitates the selection for high nutritional value. Clones with yellow roots, low cyanide level (a
common characteristic in cassava) and excellent cooking quality have been identified.

Carotenoids levels in cassava roots were measured in different plants (same clone), different roots
(same plant) and different sections of the same roots. The environmental effect on cassava was also
measured. These studies allowed the conclusion that the high-carotene trait is fairly stable. Stability of
carotenes upon different root processing methods was also measured. Boiling cassava roots will
eliminate the cyanide present in them but will retain about 60% of the original levels of carotenoids.
Furthermore, a detailed study of the quality of carotenoids in the roots revealed that more than 90% is
ß-carotene. An important finding because this particular pigment has the largest capacity to be turned
into vitamin A by the human body.

One interesting discovery was that the high-carotene trait seems to delay or reduce the onset of post-
harvest physiological deterioration (PPD) of the roots. PPD is a natural process that spoils cassava roots
one or two days after harvest. The short post-harvest storage life of cassava is a characteristic that
limits the marketability of the roots and a delayed or reduced PPD would encourage farmers to grow
yellow-rooted cassava clones.

Future Activities-The nutritional value of cassava foliage can also be exploited. In addition to the high
levels of carotenoids, it has excellent amounts of protein and minerals. One problem that the
carotenoids present in the foliage have, however, is the low bioavailability that increasing evidence is
suggesting from green vegetables.

The latest among them is the finding of scientists from the Kasetsart University, Bangkok and the
Cassava and Starch Technology Research Unit of the National Centre for Genetic Engineering and
Biotechnology, Thailand, that cassava chips are the most suitable raw material for ethanol production.
The production cost and time can be minimised through the simultaneous saccharification and
fermentation process as already implemented in bioethanol production from cereal grains.

Thailand produces about 20 million ton of cassava a year. Researchers have found that cassava was a
better feedstock to produce daily the required two million litre of ethanol for its 10% fuel substitution
plan. About 80-90% of the roots are consumed by starch and the chip and pellet industry. The balance
is available for ethanol production, unlike sugarcane or molasses, which are in short supply in Thailand.

The thermal properties of seven commercial modified cassava starches, including oxidized, acetylated,
cross-linked, and combined acetylated and cross-linked starches were studied by differential scanning
calorimetry (DSC) in the glassy and rubbery states. Increase in gel hardness in the rubbery state during
storage was also monitored, as well as gelatinization behavior. The modified starches were prepared
from granular starch and had a degree of substitution in the range 0-0.053. The glass transition
temperatures (Tg) of the modified starches were 3-6°C significantly lower than that of the non-
modified starch.
The physical aging peak temperatures were also significantly reduced by 2-3°C, compared to the non-
modified starch, while aging enthalpies increased. Starch modifications did not decrease amylopectin
retrogradation significantly. During storage, the oxidized starch gel became significantly harder than
the non-modified starch gel, while the hardness of the acetylated and/or cross-linked starch gels was
significantly reduced, which confirmed that acetylation or cross-linking can decrease hardness, even
when the extent of modification is limited. Different modifications controlled different properties of
the starch system, with cross-linking and acetylation influencing the gelatinization behavior and the
changes in starch gel texture during storage, respectively.

Cassava chain development in West Africa

Goal-To improve food security, raise rural incomes and permit the transition to sustainable market-
driven farming systems and supply lines

Purpose-To build prototype competitive and market-based cassava commodity chains by solving key
bottlenecks

Description-The agribusiness sector is hardly using cassava as a raw material (less than 1%). Processing
is limited to very few hands, with less efficient equipment, low product quality, and no product
diversification. Though traditional cassava products will continue to dominate West African cassava-
marketing channels for the foreseeable future, West African governments see the development of
industrial markets for cassava products as a new opportunity for their farmers and as an engine of
economic growth.

The proposed project therefore aims at adding value to cassava production in order to increase the
incomes of producers and small-scale processors, and to enhance the accessibility of safe cassava
products for consumers. The overall goal of the project is to contribute to sustainable improvements in
the welfare and livelihoods of farmers, processors in the cassava sector, raising incomes of farmers,
processors and local marketers in selected areas in Sierra Leone, Benin, and Nigeria, and thereby also
increasing food security.

The project purpose is to develop competitive cassava commodity chains for a reliable supply of
processed products for food and non-food industrial use, by upgrading and expanding traditional
processing techniques for making regionally widely accepted traditional products, and developing the
high quality cassava flour (HQCF) supply chain in Sierra Leone, Benin, and Nigeria.

The main theme of the project is the addition of value to cassava production in order to increase the
incomes of farmers and small to medium scale processors, and to enhance supply of safe cassava
products for consumers. The three project components are: A: Development of supply lines for high
quality cassava flour (HQCF) for bakery and confectionary markets B: Upgrading traditional cassava
processing plants for defined markets C: Project coordination and backstopping, monitoring and
evaluation, exchange and dissemination of results

The project focuses on small and medium scale enterprises and associations of processors as a means of
linking producers and processors with market demand. The project will use public-private sector
partnerships to develop the cassava sub-sector in the region. The regional dimension of the project will
enhance exchange of experiences. Small and medium scale enterprises are expected to benefit from
increased access to knowledge and services. It is expected that the development of appropriate
prototypes of cassava processing units (3 SMEs and 8 MPCs) coupled with various capacity buildings on
cassava production, processing and enterprise development will lead to higher revenues and better
working conditions for the labor force involved, which is largely composed of women (over 80% in
cassava processing centers throughout West Africa).
Farmers and processors will benefit from structured supply lines. Farmers will receive training on the
application of new disease resistant and high yielding IITA varieties. End users will benefit from access
to affordable products of good quality

Potential impacts/potential beneficiaries- Target Group: The private sector stakeholders to be
targeted will be small and medium size. (Typical income in traditional processing is between $1-
2/day.) The main characteristic is that they are existing enterprises, reliable, willing to be part of a
participatory experiment and willing to show and train others without Benefit: They should be located
in the dryer Northern or middle part of the countries where the supply and price of roots, and sun-
drying conditions are favourable. - In Sierra Leone, the program will be dealing with small private gari
units, with a present production of some 100/500 kg/day.

Further, it is envisaged to include UPWARDS, a local NGO in the North, that can be seen as a medium
to large commercial enterprise, as they cultivate some 1500 ha of cassava, but they also involve some
500 farming families.

Capacity building is in their mandate. - In Benin, the private sector is engaged through the
international NGO VECO-Benin, who is working with village associations of processors, small rural
enterprises and local bakeries. - In Ghana, cassava processing is already more advanced. Private sector
partners will mainly be existing small and medium size cassava processing enterprises, owned by
individuals or in some cases by groups

Expected results- Appropriate and profitable drying technology is developed and promoted. Supply
lines for HQCF are being developed Appropriate prototype plants for production of traditional cassava
products have been designed, validated by users and promoted Pilot supply lines and initiatives for use
of cassava in the feed market are being developed Appropriate technology for utilisation of cassava in
the domestic feed industry is being tested. Project managed and coordinated effectively and
efficiently. Results and experiences exchanged and disseminated

Great Lakes Cassava Initiative

Goal-To strengthen the capacity of 60 partners to prepare for and respond to the present cassava
mosaic disease and emerging cassava brown streak disease pandemics that threaten food security and
incomes of cassava

Purpose-To strengthen the capacity of 60 local African partners and approximately 1.15 million
farmers within four years to address cassava mosaic disease and the emerging cassava brown streak
pandemics that threaten food security and incomes of cassava dependent farm families in Burundi,
Democratic Republic of Congo, Kenya, Rwanda, Tanzania and Uganda

Description-Over four years, the Great Lakes Cassava Initiative will strengthen the capacity of 60
partners to prepare for and respond to the present cassava mosaic disease and emerging cassava brown
streak disease pandemics that threaten food security and incomes of cassava dependent farm families
in six Great Lake countries: Burundi, Democratic Republic of Congo, Kenya, Rwanda, Tanzania and
Uganda.

Catholic Relief Services, with the International Institute for Tropical Agriculture, will develop
sustainable capacity in the region to diagnose and monitor the diseases and predict their spread.
Linking with existing breeding programs, farmer evaluation of new varieties and integrated crop
management options, decentralized seed systems and farmer training involving over 4,100 farmer
groups will increase yields by 50% for approximately 1.15 million farm families (approaching 7 million
persons), generating $58 million in revenue.
This will enable farmers to exploit emerging market opportunities in collaboration with the Natural
Resources Institute Cassava Adding Value for Africa. Engaging with and supporting Pan African, regional
and country cassava initiatives, the Great Lakes Cassava Initiative will lay the foundation for going to
scale in these six countries and expanding into Southern and West Central Africa.

Cassava R&D has in the recent past received substantial impetus with impressive research findings,
significantly enhanced support from traditional sources of funding like Rockefeller Foundation, USAID,
Danida and others. New sources of funding like the Gates Foundation support the Harvest Plus and the
BioCassava Plus projects while the Cassava Genome Sequencing project is being funded by the US Dept
of Energy. The GCP is convening its first scientific meeting, GCP-I, to raise awareness of the
importance of the cassava crop in the world, to review recent scientific progress, identify and set
priorities for new opportunities and challenges as well as chart a course for seeking for support for
areas of cassava R&D for which support is currently inadequate or lacking.

Scientists have determined how to fortify the cassava plant, a staple root crop in many developing
countries, with enough vitamins, minerals and protein to provide the poor and malnourished with a
day's worth of nutrition in a single meal.

The researchers have further engineered the cassava plant so it can resist the crop's most damaging
viral threats and are refining methods to reduce cyanogens, substances that yield poisonous cyanide if
they are not properly removed from the food before consumption. The reduction of cyanogens also can
shorten the time it takes to process the plant into food, which typically requires three to six days to
complete. Studies also are under way to extend the plant's shelf life so it can be stored or shipped.

The international team of scientists hopes to translate the greenhouse research into a product that can
be field tested in at least two African nations by 2010. Funded by more than $12.1 million in grants
from the Bill & Melinda Gates Foundation, the group of researchers is led by Richard Sayre, a professor
of plant cellular and molecular biology at Ohio State University.

"Some biofortification strategies have the objective of providing only a third of the daily adult nutrition
requirements since consumers typically get the rest of their nutritional requirements from other foods
in their diet. But global food prices have recently gone sky high, meaning that many of the poorest
people are now eating just one meal a day, primarily their staple food.

The roots can be banked in the ground for up to three years, providing food security, but the plant
must undergo time-consuming processing immediately after harvest to remove compounds that
generate cyanide. Unprocessed roots also deteriorate within 48 hours after harvest, limiting the food's
shelf life. And a plant disease caused by the geminivirus reduces yields by 30 percent to 50 percent in
many areas in sub-Saharan Africa, a major blow to farm productivity.

Sayre and colleagues from multiple institutions set out to tackle virtually all of cassava's problems to
make the plant more nutritious and to increase the crop's revenue-producing potential for farmers.
Sayre reported that the research team has been able to address each of the plant's deficiencies in
individual transgenic plants. The next step will be to combine some or all of the bioengineered traits
into a single, farmer-preferred cultivar, with the goal of eventually developing cassava varieties that
carry all of the improvements developed by the researchers.

"We've begun field trials in Puerto Rico to make sure the plants perform as well outside as they do in
greenhouses, and we hope to start field trials in the target countries of Nigeria and Kenya by 2009,"
Sayre said.

The labs in the project have used a variety of techniques to improve on the model cassava plant used
for the research. They used genes that facilitate mineral transport to produce a cassava root that
accumulates more iron and zinc from the soil. To fortify the plants with a form of vitamin E and beta-
carotene (also called pro-vitamin A because it converts to vitamin A in the body), the scientists
introduced genes into the plant that increase terpenoid and carotenoid production, the precursors for
pro-vitamin A and vitamin E. They achieved a 30-fold increase in pro-vitamin A, which is critical for
human vision, bone and skin health, metabolism and immune function.

Adding protein to the cassava plant has posed a challenge, Sayre said. The scientists discovered that
most of the nitrogen required to make the amino acids used for protein synthesis in roots is derived
from the cyanogens that also cause cyanide toxicity. So their strategy for increasing protein levels in
roots focuses on accelerating the conversion of cyanide-containing compounds into protein rather than
completely eliminating cyanogen production, which would hinder the efforts to increase protein
production, Sayre explained. To further address the cyanide problem, the scientists have also
developed a way to accelerate the processing methods required to remove cyanide -- a days-long
combination of peeling, soaking and drying the roots before they are eaten.

To strengthen the cassava plant's resistance to viruses, the scientists introduced a protein and small
interfering RNA molecules that interfere with the viruses' ability to reproduce.

Prolonging cassava's shelf life has involved the development of a hybrid species that crosses two
related plants native to Texas and Brazil. The strategy, still in development, will combine the
properties of these plants and additional genes that function as antioxidants, slowing the rotting
process that has been traced to the production of free radicals that damage and kill cells in newly
harvested cassava roots.

The first cassava product the team plans to develop for investigations in the field will likely include the
virus resistance, elevated protein, elevated beta-carotene (pro-vitamin A) and elevated minerals (iron
and zinc), Sayre said. "These traits have been working the best in the greenhouse, and the virus
resistance is critical to success in the field," he said. "The thinking behind starting with these four traits
is driven by science and by the impact they can have."

"It will not only be an improved staple crop eaten as a main source of nutrition, but we're also looking
at the transformation of cassava from a staple crop to an income-generating crop," Sayre said. "That
lifts people out of poverty, allows families to send kids to school and build infrastructure in their
villages, so this is an important way to cross cultural barriers. There are many different cultures and
languages in Africa, but higher crop yield, productivity, longer shelf life and making money are things
that everyone understands."

The impetus for the genome sequence began in 2003 with the formation of The Global Cassava
Partnership (GCP-21), co-chaired by Dr. Claude Fauquet, director of the International Laboratory for
Tropical Agriculture Biology (ILTAB) at the Donald Danforth Plant Science Center (DDPSC) in St. Louis,
and Dr. Joe Tohme of the International Center for Tropical Agriculture (CIAT) in Cali, Colombia. This,
in turn, led to a 2006 proposal by Fauquet, Tohme and 12 other international scientists to DOE JGI's
Community Sequencing Program, which was selected for a pilot project.

The full genome project gathered momentum in early 2009 when 454 Life Sciences and DOE JGI each
pledged the resources to use 454's Genome Sequencer FLX platform with long-read GS FLX Titanium
chemistry to rapidly generate the DNA sequence data needed for the project.

"This is a perfect example of how quickly things can happen when everyone is aligned behind an
important cause. Most of the data for the genome were generated within 8 weeks of getting DOE JGI
and 454 Life Sciences on board," said the UA's Rounsley, who led the collaboration.

More than 61 million sequencing reads were generated and assembled into a draft genome that
contains an estimated 95 percent of cassava genes. It is one of the first large genome projects to
primarily use 454 Life Sciences' long-read sequencing platform, which enabled both improved quality of
the draft, and its rapid generation.

"We are pleased to contribute our sequencing technology to this important global initiative," explained
Michael Egholm, Chief Technology Officer and Vice President of Research and Development at 454 Life
Sciences. "This project, along with other recently completed complex plant genome projects,
demonstrates that 454 Sequencing systems are rapidly becoming the standard for de novo sequencing
and assembly."

The availability of the genome sequence enables the newly-funded project to study how cassava
varieties differ from each other. "The contributions of 454 Life Sciences and DOE JGI in making the
cassava genome a reality have opened a new chapter in cassava research worldwide. We're excited
about the opportunity for cassava breeders to access new tools for improving a staple African crop,"
said Katherine Kahn, program officer with the Agricultural Development initiative at the Bill & Melinda
Gates Foundation.

Researchers will use next-generation technologies to sample many varieties of cassava and develop a
large database of markers that can be used to identify genes involved in many important traits. The
team will collaborate with researchers in Kenya, Uganda and Tanzania in applying these genetic
markers toward identifying resistance to Cassava Brown Streak Disease. All of the information and tools
the project develops will be freely available worldwide.

Traditional cassava improvement is slow and difficult. The availability of large numbers of markers will
help make breeding schemes more efficient. For instance, traits that may only show up in mature
plants can be identified in seedlings with a cheap DNA test. Since cassava is used for industrial starch
production, and has potential as a biofuel source, there are commercial applications of these breeding
tools. However, the most important applications will be those that improve the lives of those who
depend upon cassava for their daily calorie intake.

"With the first cassava genome in hand, we can cheaply and quickly sequence other varieties that will
give us thousands of little signposts – mile markers if you like – that will help us identify key genes for
increasing the plant's resistance to the virus," Rounsley said.

"The information contained in the cassava genome will provide tremendous opportunities to improve
this important crop, bringing it into the mainstream of plant research thereby reducing the time and
cost of delivering improved cultivars to farmers who need it most."

Countries focused on Cassava:

In Thailand, cassava production expanded rapidly in the 1970s and 1980s in response to an ever-
increasing demand for cassava pellets used as an energy source for animal feed in Western Europe. The
country's cassava production area, initially located in southern Thailand, first moved to the eastern
seaboard provinces of Chon Buri and Rayong during the late 1970s, and in the 1980s expanded greatly
in the Northeast.

During the late 1980s, Thailand's cassava-production area covered 10 million rai. Almost all of this was
destined for the lucrative export market for cassava pellets in Europe. However, changes in the EU's
agricultural policies in 1993 lowered the support price of their own grain crops, and made Thailand's
cassava pellets no longer competitive as a cheap source of energy in animal-feed rations. Thus, the
amount of cassava pellets Thailand exported to the EU began to drop precipitously year after year and
is now less than 400,000 tons.

Foreseeing the problem of overproduction, the Thai government tried to decrease the cassava-growing
area by encouraging farmers to plant other crops, however, none of these were as well adapted to the
climatic conditions in the Northeast as cassava. As a result, farmers continued to grow cassava, albeit
in a much reduced area of about 6.2 million rai. But while the area was reduced, cassava yields started
to increase substantially from about 2.24 tons per rai in 1995 to 3.55 tons per rai in 2006/2007. The
result was that total cassava production decreased only marginally from a peak of 24 million tons in
1989 to about 16 million tons in 1998/1999 and back up to 25 million tons in 2006/2007.

So, what does Thailand do with 25 million tons of cassava roots? First, the Thai cassava industry quickly
changed from making mainly cassava pellets for export to making more and more cassava starch for
both the domestic and export markets. Currently the cassava starch and modified starch industry
absorbs over 50 per cent of all cassava roots produced in the country, as compared to 36 per cent in
1991. Secondly, our Chinese neighbours to the north have also built more and more starch factories, to
the point that domestic production could not keep up with demand. Thus, in 2001, they started
importing dry cassava chips from Thailand, first in very modest amounts, but increasing every year to
four million tons in 2006.

Finally, in 2000, Thailand was one of the first countries in Asia to initiate a "gasohol" or E10
programme, with the aim of replacing 10 per cent of normal gasoline with fuel-ethanol, which is a
renewable energy source made from locally produced sugarcane (or molasses), maize or cassava.

Presently 15 factories produce a total of 3.4 million liters of ethanol per day. Thailand currently has
the second highest cassava yield after India and nearly double the average yield in the world. The rapid
increase in the country's cassava yield was achieved through the hard work and excellent collaboration
among the Agriculture Department, the Agriculture Extension Department and Kasetsart University as
well as with the private processing and trading sector and the Thai Tapioca Development Institute.

So what does the future hold for cassava in Asia? In many countries the increasing demand for cassava
roots can only be satisfied through marked increases in yield. This will require renewed efforts in
breeding, agronomy, biotechnology and improvements in processing technologies, coupled with a
dynamic and effective extension programme using a farmer participatory approach. Even though
cassava is the third most important food crop in Southeast Asia after rice and maize, it has always been
considered as an "orphan crop", with little funding allocated for research of the crop. While there are
thousands of researchers all over the world working on important crops like rice, maize, soybean, oil
palm and rubber, there are only a few dozen researchers working on cassava. Unless this situation
improves and the crop receives adequate funding and research attention, it will remain an "orphan
crop", only grown by the poorest farmers and eaten by the poorest people, except that the increased
demand for fuel-ethanol, if not met through rapid increases in production, will push up the price until
the poor will no longer be able to afford it.


In Venezuela, a team of researchers on the Yaretanol project report that they have developed a
means to convert cassava waste to ethanol, butanol, isobutanol and propanol. “Yare” is the local term
for the milky byproduct of cassava root food production, a substance high in cyanide content. The
team said that 2 cubic centimeters of Yare can kill an animal weighing over 1,000 pounds. The Yare
conversion process, according to the research team could produce 1 percent of Venezuela’s ethanol, or
3 Mgy. The group also reported that they can use starch and ethanol as a base for biopolymers, and
plastic extract as a base for bio-combustibles.

China-based Hainan Yedao Group has reportedly invested $51.5m (£31.8m) in a new biofuel facility
that is expected to produce 33 million gallons a year of bio-ethanol from cassava plants. The plans
follow recent moves from the Beijing government to ban the use of grain-based energy crops in bio-
ethanol, amid concerns demand had led to a decline in food supplies.

Cassava avoids the ban as it is a root vegetable that represents China's fifth-largest crop yield after
rice, sweet potato, sugar cane and maize. The Guangxi region, next to where the plant is located,
accounts for 70 per cent of the country's yield, averaging seven million tons a year.
Traditionally ethanol produced from cassava is used for food and pharmaceutical purposes, but it is
increasingly being touted as a more sustainable alternative to first-generation biofuels. China's Beihai
Gofar Marine Biological Industry has also announced plans for a 100,000 ton-per-year cassava-based
ethanol plant in the Guangxi region. The government has also signalled its support for the biofuel
sector, with 10 of the country's 22 provinces in China mandating the use of ethanol-blended gasoline in
cars. China will build its largest production base of non-grain bio-ethanol fuel in a southern province to
mix with or replace the gasoline and diesel oil for vehicle use, reported Shanghai-based Jiefang Daily
last week. Global automakers have seen the promise of the new-generation ethanol fuel development
in China.

In April, China successfully replaced the gasoline and diesel oil with bio-ethanol fuel in Guangxi Zhuang
Autonomous Region, and gas stations in 14 cities of Guangxi started to sell bio-ethanol. Some locally
produced fuel ethanol is also mixed in a ratio of 10% with the ordinary gasoline to produce ethanol
gasoline. The large ethanol refiner of Guangxi's Beihai city now can produce 200,000 tons of ethanol
annually out of about 1.5 million tons of cassava.

Guangxi is the first Chinese province to use cassava instead of grain to produce ethanol. Guangxi's
output of cassava accounted for more than 60% of the country's total, with an output of 7.8 million tons
a year. China has forbidden using grain for ethanol production last year to guarantee the supply of
food. Now, about ten other Chinese provinces are using ethanol fuel. The country's ethanol fuel sales
will reach 30 million tons in 2010 to make up half of the total gasoline supplies.

Ethanol fuel will help China, the world's second largest auto market, ease the shortage of energy
supply, and cut the carbon monoxide and carbon dioxide emissions by about 30% and 10% respectively.
China may have 60,000 new energy vehicles by 2012 and the government will focus on boosting the
annual production of clean-energy vehicles.

Agriculture remains the main activity in the developing world. The world’s farmers are fully capable of
increasing the amount they produce, but, in order for this to happen, demand must increase. Poverty
reduces global food demand and acts as a brake on food production. Most of the world’s poorest people
live in rural areas and work the land. Increased demand for agricultural products thus would mean
more farm income and less poverty, more productive agriculture, more food, more rural economic
development, and reduced migration to cities.



Conclusion : Many developing countries still have a significant quantity of land available that is well
adapted to rain-fed crops – about as much as now is being farmed (over 1.7 billion acres). These lands
do not include areas inhabited by human beings, forests, or protected areas.

If a country can produce and export biofuels, it will have a stronger economy and more resources to
address the needs of the poor. Africa, with its significant sugar cane/Cassava production potential, is
often cited as a region that could profit from experience and technology, although obstacles to
realizing it (infrastructure, institutional, etc.) should not be underestimated.

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Cassava Study: Biomass Potential for Energy

  • 1. Cassava Study: One recent study concluded that by 2050, biomass theoretically could supply 65% of the world’s current energy consumption, with sub-Saharan Africa, the Caribbean, and Latin America accounting for roughly half of this global potential. In tropical countries, high crop yields and lower costs for land and labor provide an economic advantage that is hard for countries in temperate regions to match. Cassava has erupted into the first decade of the third millennium as a crop that can contribute to agro- industrial and small-farmer development in the tropics. Cassava is progressively shifting roles from being a staple food for human consumption to becoming an efficient industrial crop particularly in developing economies of Asia, Latin America, and Africa. Cassava owes part of its popularity to its high rate of conversion of solar energy into starch per unit area, as compared to other starchy staple crops e.g., rice and maize. In addition to the high carbohydrate content (74 - 85% of its total storage root dry weight), it contains varying amounts of vitamin C (ascorbic acid), vitamin A (carotenes), iron, zinc, calcium, potassium and protein that account for its wide popularity . To a large extent, the difference between the progression of cassava starch-based industries in the ACP region and Asia is the differential Government commitment towards cassava commercialization and increasing investment in research and development. The livestock sector has also been targeted for utilisation of cassava either through minimal processing i.e., silage or use of well-refined feed rations. Equally important is the need to undertake strategic research to identify, develop and promote cassava varieties that can meet animal feed standards and satisfy the needs of small herders as well as large livestock producers for high quality feed at affordable prices. Together, these initiatives will increase the utilisation of cassava in animal feed and hence increase the profitability of the livestock sector in the ACP region. In Africa, most cassava research is geared towards addressing key production and post-harvest constraints, with limited efforts devoted towards increasing the competitiveness of cassava in the industrial sector. Nevertheless, some strategic pioneering research is underway in different parts of the continent. For example, in partnership with the Swedish University of Agricultural Science, Uganda has embarked on starch metabolism studies Also, the International Institute of Tropical Agriculture (IITA), based in Nigeria, is having special interest in: 1. enhancing beta carotene, zinc (Zn), iron (Fe) and protein levels in cassava; 2. genetic modification of cassava starch functionality and; 3. Development of cassava varieties with varied starch content and profiles. It is rather surprising to note that despite the huge acreage of cassava in Africa, Government commitment to this crop varies considerably between countries. For example, Ghana and Nigeria stand out as countries on the continent that have witnessed full and genuine presidential support for promotion of cassava starch. The above selected examples strongly illustrate existing opportunities that can permit the transformation of cassava from being a staple food crop to an industrial crop in the ACP region, as has been witnessed in Asia and Latin America. These opportunities are broadly characterized as: 1. cassava being irreplaceable by other starches i.e., cassava starch as the best; 2. cassava is rated poor as compared to other starch crops and; 3. Cassava is easily interchangeable with other starches.
  • 2. Cassava starch utility: Food products- Native tapioca starch is widely applied in food recipes such as bakery products. It is also used to produce extruded snacks and tapioca pearls. Modified starch, or starch derivatives, has been applied as thickening, binding, texturizing and stabilizing agents.Uses as fillers, sweeteners, flavor carriers and fat replacement in many food products include canned food, frozen food, dry mixes, baked goods, snacks, dressings, soups, sauces, dairy products, meat and fish products and infant food. Beverage- Modified tapioca starch is used as a colloid stabilizer in beverages that include solid constituents. Tapioca starch-based sweeteners can be produced with considerably higher yields than sugar and are used in beverages as a sugar replacement. In combination with other sweetener components, it can usually contribute to satisfying the customer’s requirement. High dextrose equivalent syrups of tapioca-based hydrolysate are also good sources of easily fermentable sugars for brewery applications. Confectionery- Native tapioca and diverse types of modified tapioca starch are used in confectionery for different purposes such as gelling, thickening; texture stabilizing, foam strengthening, crystallization inhibition, adhesion, film forming, and glazing. Low viscosity tapioca starches are widely used in gelled confectioneries such as jellies and gums. The most often used one is acid-thinned starch due to its high retro-gradation and gel formation characteristics, which are enhanced by the presence of sugars. Powdered starches are used as mould release agents when casting confectioneries. Starch- based polyols make the manufacture of sugar-free chewing gum possible. Chemicals- Tapioca starch-based syrups are obtained economically by acid and/or enzyme processes and used as feedstock to make various chemicals, including monosodium glutamate, amino acids, organic acids, alcohols, ketones, vitamins and antibiotics. Production techniques include chemical reaction, fermentation and other biotechnological processes. Adhesives & Glue- Tapioca starch-based dextrins are excellent adhesives and used in many applications including corrugated board, paper-bags, laminated board, gummed paper, tapes, labels, stamps and envelopes. Paper- Modified tapioca starches are applied in the paper industry to improve paper quality, increase production rates, and improve pulp yield. Cationic starches are employed to flocculate pulp, increasing de-watering rates on the wet end. Faster machine speeds and better pulp yields result. The starch remains in the finished paper, acting as an internal sizing agent to increase the paper strength. Low viscosity starches, such as oxidized starches, are applied as surface sizing to improve the strength and control ink absorption properties for printing and writing. Modified tapioca starches are also used as pigment binder for surface coating to obtain a smooth, white paper. Textile- Tapioca starches are used in the textile industry as sizing agents to stiffen and protect the thread for improved weaving efficiency. They are also used as finishing agents to obtain smooth fabrics, and color thickeners to obtain sharp and durable printed fabrics. For this purpose, thin-boiled starches are usually preferred. Pharmacy and Cosmetics- Native and modified tapioca starches are used as binders, fillers and disintegrating agents for tablet production. Specialty modified starches are used as a carrier for skin moisturizers, which are frequently mineral oil based. Other modified starches are used as emulsifiers, encapsulating agents (vitamins), sizing (mousse for hair), thickeners (shampoo), etc. Biodegradable materials- Native and modified tapioca starches can be blended with petroleum-based or synthetic polymers to improve the biodegradability and minimize the production cost of more environmentally friendly materials.
  • 3. Applications of modified tapioca starch in food industry- Starch type Functionality/Property Application- Pre-gelatinized starch Thickening, Cold water Instant soups, instant soluble puddings, sauces, bakery mixes, frozen food Acid-thinned starch Lower viscosity, High Gum, candies, formulated retrogradation, Strong gel liquid food Dextrins Binding, coating, Confectionery, baking,encapsulation flavorings, spices, oils Oxidized starch Stabilizer, adhesives, Formulated food, batter,gelling, clarifying agent gum, confectionery Starch ethers Stabilizer, fat replacement Soups, puddings, frozen(Hydroxy-alkyl starch food Carboxy-methyl starch)Starch ester Stabilizer, thickening Candies, emulsion (Acetylated starch agent, clarification Phosphate mono-ester starch)Cross-linked starch Thickening, stabilizer, Pie fillings, breads, frozen (Di-starch phosphate) texturizing agent products, bakery, pudding,instant foods, soups,gravies, salad dressing. More than 228 million tons of cassava was produced worldwide in 2007, of which Africa accounted for 52%. In 2007, Nigeria produced 46 million tons making it the world's largest producer. According to 2002 FAO estimates, Africa exports only one ton of cassava annually. Cassava production depends on a supply of quality stem cuttings. The multiplication rate of planting materials is very low compared to grain crops, which are propagated by true seeds. In addition, cassava stem cuttings are bulky and highly perishable as they dry up within a few days. Harvesting - Nineteen million hectares of cassava were planted worldwide in 2007, with about 63% in Africa. Cassava requires less labor than all other staple crops (21% in working days as compared to maize, yam and rice). However, it requires considerable postharvest labor because the roots are highly perishable and must be processed into a storable form soon after harvest. Roots can be harvested between six months and three years after planting. Consumption- Nearly every person in Africa eats around 80 kilograms of cassava per year. It is estimated that 37% of dietary energy comes from cassava. The Democratic Republic of Congo is the largest consumer of cassava in SSA, followed by Nigeria. Disease and constraints- Major pests of cassava in SSA are the cassava green mite and the variegated grasshopper. The main diseases affecting cassava are cassava mosaic disease (CMD), cassava bacterial blight, cassava anthracnose disease, and root rot. CMD alone accounted for an estimated 47% of East and Central Africa's cassava production losses during a serious outbreak beginning in the early 1990's until 2006. Pests, disease and poor cultivation practices combined can cause yield losses as high as 50% in all of Africa. Opportunity in Biofuels development: Biofuels offer Africa the chance to supply itself with alternative energy sources, and also to become a major supplier of these sources for developed markets. Yet, challenges - from creating the relevant infrastructure to competition for biofuels crops from food markets - remain. It is rather surprising to note that despite the huge acreage of cassava in Africa, Government commitment to this crop varies considerably between countries. For example, Ghana and Nigeria standout as countries on the continent that have witnessed full and genuine presidential support for promotion of cassava starch. The above selected examples strongly illustrate existing opportunities that can permit the transformation of cassava from being a staple food crop to an industrial crop in the ACP region, as has been witnessed in Asia and Latin America. These opportunities are broadly characterized as: 1. cassava being irreplaceable by other starches i.e., cassava starch as the best; 2. cassava is rated poor as compared to other starch crops and; 3. Cassava is easily interchangeable with other starches.
  • 4. Utilizing biomass to produce biofuels and chemical products is an important direction for realizing sustainable development. Biomass is a kind of renewable resource, but it is also significant to promote an ecological development pattern in Biorefinery processes by raising resource efficiency, reducing consumption of energy and water, decreasing waste emission as well as constituting industrial symbiosis products network. Thus, it is possible to reach overall sustainable development of the Biorefinery industry. Different biomass raw materials such as corn, wheat, sweet potato, cassava and oil plants are fermented to produce different kinds of products including fuel ethanol, biodiesel, 1,3-propanediol, butanediol, acetone, butanol, lactic acid and dry barm. And bioethanol will be converted into bioethene in the next stage. The key to cassava's future in global and domestic starch markets will be improvements in efficiency and quality, and a reduction in production costs. For a model of successful cassava starch industry development, African and Latin American countries need look no further than Thailand, the world's No. 1 producer. The Thai industry began more than 50 years ago, and expanded rapidly during the 1990s, when trade restrictions sharply reduced the European market for Thai dried cassava chips, used as animal feed. Thailand now uses about 50 percent of its annual cassava root production, of around 18 million tons, to extract some two million tons of starch. Half of it is destined for domestic food and non-food industries, the rest being exported, mainly to Japan and Taiwan, and increasingly in the form of higher-value modified starch for specialized applications. Thailand is the world’s largest exporter of tapioca starch and starch derivatives, with annual production of over 2 million tons of starch. The country is also exploring a promising new market for its starch - as raw material for production of ethanol used as a biofuels. The leading Thai petroleum company has announced a feasibility study for a plant that would use cassava to produce one million litres of ethanol per day. Fledgling cassava starch industries concentrate initially on meeting domestic demand - a study of global cassava markets found that tropical countries were importing annually maize starch and derivatives to a value of more than $80 million. In many countries, the study found, almost all imports could be replaced with locally-made cassava starch or, for simple applications, even by good-quality cassava flour. In Africa, there are signs of growing interest in using locally-made cassava starch as an import substitute. Cassava starch start-ups have recently been established in Uganda, Tanzania, and Madagascar, while in Malawi industries have shown interest in buying local cassava starch for use in paper, cardboard, sweets and food processing. Meanwhile, the region's leading cassava producer, Nigeria, has recently announced an ambitious programme aimed at producing ethanol biofuel from cassava. With better farmer management, we can produce an average of 30 to 50 tonnes of cassava per hectare as opposed to the 5 tons per hectare from local varieties commonly used by farmers in these areas. In addition, opportunities exist for African-produced biofuels to enter the U.S. market under the African Growth and Opportunities Act, Washington’s answer to EBA. Import markets are developing in other major global economies like Japan, and a handful of African countries are looking at biofuel use as well. The strategies established in the main four countries are also laying down guidelines for tax breaks and at times guaranteeing prices for producers and establishing regulatory structures. Many of Africa’s most viable biofuel producers - in terms of production costs for sugarcane, maize or cassava - are landlocked, however, and don’t have the transportation infrastructure in place to enable export of the fuels, or immediate plans for infrastructure investment like Mozambique. Several countries in Africa are developing biofuel policies to help state-owned and commercial companies alike bring about biofuel production, and some are creating local markets. Small countries like Rwanda,
  • 5. which are not only landlocked but suffer from high petroleum import costs, are looking at biodiesel production for the local market. Although production of biofuels in West Africa may at present be insignificant compared to the rest of the world market, the countries in the region seem determined to exploit what they see as a godsend of a market. A recent report by the OECD shows that the West African nations are viewing this new market as a promising way of boosting their own standing and enhancing their economies. In almost all West African countries, the new biofuels business is being negotiated by and carried out by large multi nationals. These countries are seen as rich resources to be exploited, because of their potential to grow biofuel crops. They have vast swathes of unused agricultural land that has high potential to grow the raw materials for biofuels. The OECD says that this potential for exploitation by the multinationals and the potential for growth in the biofuels industry in West Africa present several challenges to the countries. It says they have to ensure that land competition with land used for food purposes is avoided and that natural capital is observed. The OECD says they must "negotiate 'win-win' agreements with multinationals, involving three parties: the public and private sectors as well as representatives from local communities. This would allow the best possible benefit to be obtained from the economic and social effects of producing green fuels: to ensure that sufficient income is earned, the largest possible number of local jobs is created and that the development of cooperative distilleries and/or local investment is encouraged." The OECD adds that those investing in biofuel production in these countries will naturally gravitate towards the countries where conditions are most favorable - where regulatory, environmental and ethical constraints are at a minimum. Already four countries in the region - Ghana, Nigeria, Sengal and Mali - have established national strategies for biofuels production. The national strategies have set production targets for the four countries which will see 320,000 hectares of Jatropha curcas in Senegal in 2012, a million hectares in Ghana in the medium term, 25 million litres of ethanol annually in Mali between 2008 and 2023. • Mali has also just set up a biofuels agency to centralise the government policies and set technical and quality standards for biofuel products. The National Biofuel Development Agency (ANADEB) will be responsible for overseeing the National Energy Policy and the Renewable Energy Development Strategy brought in in 2006, and last year's National Biofuel Development Strategy. However, the OECD concludes that there are genuine prospects for Africa and West Africa in particular to make the most of the situation, provided the opportunity of the new market is grasped together with the positive impacts it will have on economic growth, employment and development and at the same time while it recognizes the risks the new market will also offer. • • The Zambian Government's vision for the agriculture sector up to 2015 and beyond will be promoted taking into account the comparative advantage in crops, livestock, and fisheries production. The long term vision for the sector encompasses achieving national food security for the majority of the Zambian population through increased yields and improved post harvest management and utilisation. The Government will Endeavour to develop commercial agriculture with all the farmers producing for domestic and export markets. Promoting a competitive and efficient agriculture base on regional comparative advantage and the development of a diversified agriculture linked to a well developed agro-business industry for value adding and exports are other efforts being pursued. Notwithstanding the emphasis on development of
  • 6. small scale famers, a conducive environment is being provided for growth of large scale farming sector in order to maximize the synergies between the two. Focus will continue being on providing public goods that are needed for efficient growth such as rural infrastructure, basic research, and disease epidemic and pest control. The role of the public sector will increasingly be confined to policy formulation, enforcement of legislation, regulation, and inspection, maintenance of the national strategic food reserves. Agribusiness is being encouraged and promoted to strengthen linkages with small scale farmers through increased private sector participation in agriculture service delivery. The ministry of agriculture will also ensure increasingly commercialization and cost share some services it is currently providing. The Government will continue partnering with the private sector and Non Governmental Organisations and cooperating partners to ensure attainment of food security for the majority household with at least 90 per cent of the population being food secured. • The cassava processing industry in Indonesia is a very profitable business. Aside from the major starch product, the by-products can be used to manufacture other useful products. PT Budi Acid Jaya Tbk (BUDI) is one of the companies focusing on the production of cassava or tapioca starch in Indonesia. To take advantage of tapioca's by-product called 'onggok', the company uses it to produce higher value-added product such as citric acid. With the country's focus on biofuels, the company has planned to build a cassava-based bioethanol manufacturing plant with a capacity of 75,000 kilo liters. The plant costing US$43 million will be located in Lampung, Indonesia. Deputy President Director of BUDI, Sudarmo Tasmin, said that the development plan was a response to the promising prospect of renewable energy business and the soaring oil price which now exceeds more than US$ 100 per barel. The establishment of the plant is being conducted in collaboration with a Japanese company, to which 50% of the produced bioethanol will be sold. • The project (IFAD-ICRISAT) facilitates entrepreneurs to utilize sweet sorghum stalks and cassava roots in producing ethanol, and seeds of jatropha in producing bio-diesel. The above program will be implemented by sensitizing farmers, research partners and other stakeholders in the production and supply chain about biofuel production. This will enable them to work together and make use of the project's research outputs, such as, improved target crop cultivars, production packages, seed systems, processing technologies (including management of effluents and exploitation of by-products), and learn about innovative input and market linkages developed for different agro-eco-regions in the target countries. In addition, the project draws upon the strength of small-scale farmers' know-how in formulating and implementing various activities. The overall purpose of the project is thus to facilitate small-scale farmers and landless poor to take advantage of the market demand for their crops for bio-fuel production and/or utilize the bio-fuels for local use (e.g. running motor pump), which in turn, will help them improve their livelihoods and rehabilitate the degraded lands (wherever jatropha and local species of bio-diesel plantations are taken up). The project also envisages facilitating the development of farmer-friendly procedures to enable them to take advantage of the CDM, of the Kyoto protocol, to improve their livelihoods. The project contributes to energy self-sufficiency of the target countries. Cassava Developments: The crop produces reasonably well under marginal conditions of climate and soil and is frequently identified as a famine reserve due to its tolerance to drought and infertile soils, and its ability to recover from disease and pest attacks. It can also produce competitively in non-marginal areas. Cassava offers the advantage of a flexible harvesting date, allowing farmers to keep the roots in the ground until needed. Remarkably every part of the plant can be exploited: in addition to the roots the fresh foliage is also consumed in several regions of the world and the lignified stems are used as
  • 7. planting material. Because of its unique characteristics cassava grows in the marginal environments where poverty and malnourishment are also very prevalent. During the 1993-95 periods, about 16.5 million hectares were grown with cassava worldwide, producing 164 million tons of roots. For the period 1993 to 2020 annual growth of cassava production was estimated to range between 1.74 and 1.95% / year. The area of cassava under marginal environments has been continuously increasing, particularly for regions with poorer soils and lengthy dry seasons. Research -In the past an emphasis was given to breeding cassava with white roots. However, considerable efforts were recently invested in measuring the variability for its carotenoids contents in roots and leaves from thousands of clones, along with other relevant information. It was found that high levels of carotenoids (more than 1 and 96 mg/100 g of fresh root or leaf tissue, respectively) could be found in certain clones. High levels of carotenoids were associated with yellow coloration, which facilitates the selection for high nutritional value. Clones with yellow roots, low cyanide level (a common characteristic in cassava) and excellent cooking quality have been identified. Carotenoids levels in cassava roots were measured in different plants (same clone), different roots (same plant) and different sections of the same roots. The environmental effect on cassava was also measured. These studies allowed the conclusion that the high-carotene trait is fairly stable. Stability of carotenes upon different root processing methods was also measured. Boiling cassava roots will eliminate the cyanide present in them but will retain about 60% of the original levels of carotenoids. Furthermore, a detailed study of the quality of carotenoids in the roots revealed that more than 90% is ß-carotene. An important finding because this particular pigment has the largest capacity to be turned into vitamin A by the human body. One interesting discovery was that the high-carotene trait seems to delay or reduce the onset of post- harvest physiological deterioration (PPD) of the roots. PPD is a natural process that spoils cassava roots one or two days after harvest. The short post-harvest storage life of cassava is a characteristic that limits the marketability of the roots and a delayed or reduced PPD would encourage farmers to grow yellow-rooted cassava clones. Future Activities-The nutritional value of cassava foliage can also be exploited. In addition to the high levels of carotenoids, it has excellent amounts of protein and minerals. One problem that the carotenoids present in the foliage have, however, is the low bioavailability that increasing evidence is suggesting from green vegetables. The latest among them is the finding of scientists from the Kasetsart University, Bangkok and the Cassava and Starch Technology Research Unit of the National Centre for Genetic Engineering and Biotechnology, Thailand, that cassava chips are the most suitable raw material for ethanol production. The production cost and time can be minimised through the simultaneous saccharification and fermentation process as already implemented in bioethanol production from cereal grains. Thailand produces about 20 million ton of cassava a year. Researchers have found that cassava was a better feedstock to produce daily the required two million litre of ethanol for its 10% fuel substitution plan. About 80-90% of the roots are consumed by starch and the chip and pellet industry. The balance is available for ethanol production, unlike sugarcane or molasses, which are in short supply in Thailand. The thermal properties of seven commercial modified cassava starches, including oxidized, acetylated, cross-linked, and combined acetylated and cross-linked starches were studied by differential scanning calorimetry (DSC) in the glassy and rubbery states. Increase in gel hardness in the rubbery state during storage was also monitored, as well as gelatinization behavior. The modified starches were prepared from granular starch and had a degree of substitution in the range 0-0.053. The glass transition temperatures (Tg) of the modified starches were 3-6°C significantly lower than that of the non- modified starch.
  • 8. The physical aging peak temperatures were also significantly reduced by 2-3°C, compared to the non- modified starch, while aging enthalpies increased. Starch modifications did not decrease amylopectin retrogradation significantly. During storage, the oxidized starch gel became significantly harder than the non-modified starch gel, while the hardness of the acetylated and/or cross-linked starch gels was significantly reduced, which confirmed that acetylation or cross-linking can decrease hardness, even when the extent of modification is limited. Different modifications controlled different properties of the starch system, with cross-linking and acetylation influencing the gelatinization behavior and the changes in starch gel texture during storage, respectively. Cassava chain development in West Africa Goal-To improve food security, raise rural incomes and permit the transition to sustainable market- driven farming systems and supply lines Purpose-To build prototype competitive and market-based cassava commodity chains by solving key bottlenecks Description-The agribusiness sector is hardly using cassava as a raw material (less than 1%). Processing is limited to very few hands, with less efficient equipment, low product quality, and no product diversification. Though traditional cassava products will continue to dominate West African cassava- marketing channels for the foreseeable future, West African governments see the development of industrial markets for cassava products as a new opportunity for their farmers and as an engine of economic growth. The proposed project therefore aims at adding value to cassava production in order to increase the incomes of producers and small-scale processors, and to enhance the accessibility of safe cassava products for consumers. The overall goal of the project is to contribute to sustainable improvements in the welfare and livelihoods of farmers, processors in the cassava sector, raising incomes of farmers, processors and local marketers in selected areas in Sierra Leone, Benin, and Nigeria, and thereby also increasing food security. The project purpose is to develop competitive cassava commodity chains for a reliable supply of processed products for food and non-food industrial use, by upgrading and expanding traditional processing techniques for making regionally widely accepted traditional products, and developing the high quality cassava flour (HQCF) supply chain in Sierra Leone, Benin, and Nigeria. The main theme of the project is the addition of value to cassava production in order to increase the incomes of farmers and small to medium scale processors, and to enhance supply of safe cassava products for consumers. The three project components are: A: Development of supply lines for high quality cassava flour (HQCF) for bakery and confectionary markets B: Upgrading traditional cassava processing plants for defined markets C: Project coordination and backstopping, monitoring and evaluation, exchange and dissemination of results The project focuses on small and medium scale enterprises and associations of processors as a means of linking producers and processors with market demand. The project will use public-private sector partnerships to develop the cassava sub-sector in the region. The regional dimension of the project will enhance exchange of experiences. Small and medium scale enterprises are expected to benefit from increased access to knowledge and services. It is expected that the development of appropriate prototypes of cassava processing units (3 SMEs and 8 MPCs) coupled with various capacity buildings on cassava production, processing and enterprise development will lead to higher revenues and better working conditions for the labor force involved, which is largely composed of women (over 80% in cassava processing centers throughout West Africa).
  • 9. Farmers and processors will benefit from structured supply lines. Farmers will receive training on the application of new disease resistant and high yielding IITA varieties. End users will benefit from access to affordable products of good quality Potential impacts/potential beneficiaries- Target Group: The private sector stakeholders to be targeted will be small and medium size. (Typical income in traditional processing is between $1- 2/day.) The main characteristic is that they are existing enterprises, reliable, willing to be part of a participatory experiment and willing to show and train others without Benefit: They should be located in the dryer Northern or middle part of the countries where the supply and price of roots, and sun- drying conditions are favourable. - In Sierra Leone, the program will be dealing with small private gari units, with a present production of some 100/500 kg/day. Further, it is envisaged to include UPWARDS, a local NGO in the North, that can be seen as a medium to large commercial enterprise, as they cultivate some 1500 ha of cassava, but they also involve some 500 farming families. Capacity building is in their mandate. - In Benin, the private sector is engaged through the international NGO VECO-Benin, who is working with village associations of processors, small rural enterprises and local bakeries. - In Ghana, cassava processing is already more advanced. Private sector partners will mainly be existing small and medium size cassava processing enterprises, owned by individuals or in some cases by groups Expected results- Appropriate and profitable drying technology is developed and promoted. Supply lines for HQCF are being developed Appropriate prototype plants for production of traditional cassava products have been designed, validated by users and promoted Pilot supply lines and initiatives for use of cassava in the feed market are being developed Appropriate technology for utilisation of cassava in the domestic feed industry is being tested. Project managed and coordinated effectively and efficiently. Results and experiences exchanged and disseminated Great Lakes Cassava Initiative Goal-To strengthen the capacity of 60 partners to prepare for and respond to the present cassava mosaic disease and emerging cassava brown streak disease pandemics that threaten food security and incomes of cassava Purpose-To strengthen the capacity of 60 local African partners and approximately 1.15 million farmers within four years to address cassava mosaic disease and the emerging cassava brown streak pandemics that threaten food security and incomes of cassava dependent farm families in Burundi, Democratic Republic of Congo, Kenya, Rwanda, Tanzania and Uganda Description-Over four years, the Great Lakes Cassava Initiative will strengthen the capacity of 60 partners to prepare for and respond to the present cassava mosaic disease and emerging cassava brown streak disease pandemics that threaten food security and incomes of cassava dependent farm families in six Great Lake countries: Burundi, Democratic Republic of Congo, Kenya, Rwanda, Tanzania and Uganda. Catholic Relief Services, with the International Institute for Tropical Agriculture, will develop sustainable capacity in the region to diagnose and monitor the diseases and predict their spread. Linking with existing breeding programs, farmer evaluation of new varieties and integrated crop management options, decentralized seed systems and farmer training involving over 4,100 farmer groups will increase yields by 50% for approximately 1.15 million farm families (approaching 7 million persons), generating $58 million in revenue.
  • 10. This will enable farmers to exploit emerging market opportunities in collaboration with the Natural Resources Institute Cassava Adding Value for Africa. Engaging with and supporting Pan African, regional and country cassava initiatives, the Great Lakes Cassava Initiative will lay the foundation for going to scale in these six countries and expanding into Southern and West Central Africa. Cassava R&D has in the recent past received substantial impetus with impressive research findings, significantly enhanced support from traditional sources of funding like Rockefeller Foundation, USAID, Danida and others. New sources of funding like the Gates Foundation support the Harvest Plus and the BioCassava Plus projects while the Cassava Genome Sequencing project is being funded by the US Dept of Energy. The GCP is convening its first scientific meeting, GCP-I, to raise awareness of the importance of the cassava crop in the world, to review recent scientific progress, identify and set priorities for new opportunities and challenges as well as chart a course for seeking for support for areas of cassava R&D for which support is currently inadequate or lacking. Scientists have determined how to fortify the cassava plant, a staple root crop in many developing countries, with enough vitamins, minerals and protein to provide the poor and malnourished with a day's worth of nutrition in a single meal. The researchers have further engineered the cassava plant so it can resist the crop's most damaging viral threats and are refining methods to reduce cyanogens, substances that yield poisonous cyanide if they are not properly removed from the food before consumption. The reduction of cyanogens also can shorten the time it takes to process the plant into food, which typically requires three to six days to complete. Studies also are under way to extend the plant's shelf life so it can be stored or shipped. The international team of scientists hopes to translate the greenhouse research into a product that can be field tested in at least two African nations by 2010. Funded by more than $12.1 million in grants from the Bill & Melinda Gates Foundation, the group of researchers is led by Richard Sayre, a professor of plant cellular and molecular biology at Ohio State University. "Some biofortification strategies have the objective of providing only a third of the daily adult nutrition requirements since consumers typically get the rest of their nutritional requirements from other foods in their diet. But global food prices have recently gone sky high, meaning that many of the poorest people are now eating just one meal a day, primarily their staple food. The roots can be banked in the ground for up to three years, providing food security, but the plant must undergo time-consuming processing immediately after harvest to remove compounds that generate cyanide. Unprocessed roots also deteriorate within 48 hours after harvest, limiting the food's shelf life. And a plant disease caused by the geminivirus reduces yields by 30 percent to 50 percent in many areas in sub-Saharan Africa, a major blow to farm productivity. Sayre and colleagues from multiple institutions set out to tackle virtually all of cassava's problems to make the plant more nutritious and to increase the crop's revenue-producing potential for farmers. Sayre reported that the research team has been able to address each of the plant's deficiencies in individual transgenic plants. The next step will be to combine some or all of the bioengineered traits into a single, farmer-preferred cultivar, with the goal of eventually developing cassava varieties that carry all of the improvements developed by the researchers. "We've begun field trials in Puerto Rico to make sure the plants perform as well outside as they do in greenhouses, and we hope to start field trials in the target countries of Nigeria and Kenya by 2009," Sayre said. The labs in the project have used a variety of techniques to improve on the model cassava plant used for the research. They used genes that facilitate mineral transport to produce a cassava root that
  • 11. accumulates more iron and zinc from the soil. To fortify the plants with a form of vitamin E and beta- carotene (also called pro-vitamin A because it converts to vitamin A in the body), the scientists introduced genes into the plant that increase terpenoid and carotenoid production, the precursors for pro-vitamin A and vitamin E. They achieved a 30-fold increase in pro-vitamin A, which is critical for human vision, bone and skin health, metabolism and immune function. Adding protein to the cassava plant has posed a challenge, Sayre said. The scientists discovered that most of the nitrogen required to make the amino acids used for protein synthesis in roots is derived from the cyanogens that also cause cyanide toxicity. So their strategy for increasing protein levels in roots focuses on accelerating the conversion of cyanide-containing compounds into protein rather than completely eliminating cyanogen production, which would hinder the efforts to increase protein production, Sayre explained. To further address the cyanide problem, the scientists have also developed a way to accelerate the processing methods required to remove cyanide -- a days-long combination of peeling, soaking and drying the roots before they are eaten. To strengthen the cassava plant's resistance to viruses, the scientists introduced a protein and small interfering RNA molecules that interfere with the viruses' ability to reproduce. Prolonging cassava's shelf life has involved the development of a hybrid species that crosses two related plants native to Texas and Brazil. The strategy, still in development, will combine the properties of these plants and additional genes that function as antioxidants, slowing the rotting process that has been traced to the production of free radicals that damage and kill cells in newly harvested cassava roots. The first cassava product the team plans to develop for investigations in the field will likely include the virus resistance, elevated protein, elevated beta-carotene (pro-vitamin A) and elevated minerals (iron and zinc), Sayre said. "These traits have been working the best in the greenhouse, and the virus resistance is critical to success in the field," he said. "The thinking behind starting with these four traits is driven by science and by the impact they can have." "It will not only be an improved staple crop eaten as a main source of nutrition, but we're also looking at the transformation of cassava from a staple crop to an income-generating crop," Sayre said. "That lifts people out of poverty, allows families to send kids to school and build infrastructure in their villages, so this is an important way to cross cultural barriers. There are many different cultures and languages in Africa, but higher crop yield, productivity, longer shelf life and making money are things that everyone understands." The impetus for the genome sequence began in 2003 with the formation of The Global Cassava Partnership (GCP-21), co-chaired by Dr. Claude Fauquet, director of the International Laboratory for Tropical Agriculture Biology (ILTAB) at the Donald Danforth Plant Science Center (DDPSC) in St. Louis, and Dr. Joe Tohme of the International Center for Tropical Agriculture (CIAT) in Cali, Colombia. This, in turn, led to a 2006 proposal by Fauquet, Tohme and 12 other international scientists to DOE JGI's Community Sequencing Program, which was selected for a pilot project. The full genome project gathered momentum in early 2009 when 454 Life Sciences and DOE JGI each pledged the resources to use 454's Genome Sequencer FLX platform with long-read GS FLX Titanium chemistry to rapidly generate the DNA sequence data needed for the project. "This is a perfect example of how quickly things can happen when everyone is aligned behind an important cause. Most of the data for the genome were generated within 8 weeks of getting DOE JGI and 454 Life Sciences on board," said the UA's Rounsley, who led the collaboration. More than 61 million sequencing reads were generated and assembled into a draft genome that contains an estimated 95 percent of cassava genes. It is one of the first large genome projects to
  • 12. primarily use 454 Life Sciences' long-read sequencing platform, which enabled both improved quality of the draft, and its rapid generation. "We are pleased to contribute our sequencing technology to this important global initiative," explained Michael Egholm, Chief Technology Officer and Vice President of Research and Development at 454 Life Sciences. "This project, along with other recently completed complex plant genome projects, demonstrates that 454 Sequencing systems are rapidly becoming the standard for de novo sequencing and assembly." The availability of the genome sequence enables the newly-funded project to study how cassava varieties differ from each other. "The contributions of 454 Life Sciences and DOE JGI in making the cassava genome a reality have opened a new chapter in cassava research worldwide. We're excited about the opportunity for cassava breeders to access new tools for improving a staple African crop," said Katherine Kahn, program officer with the Agricultural Development initiative at the Bill & Melinda Gates Foundation. Researchers will use next-generation technologies to sample many varieties of cassava and develop a large database of markers that can be used to identify genes involved in many important traits. The team will collaborate with researchers in Kenya, Uganda and Tanzania in applying these genetic markers toward identifying resistance to Cassava Brown Streak Disease. All of the information and tools the project develops will be freely available worldwide. Traditional cassava improvement is slow and difficult. The availability of large numbers of markers will help make breeding schemes more efficient. For instance, traits that may only show up in mature plants can be identified in seedlings with a cheap DNA test. Since cassava is used for industrial starch production, and has potential as a biofuel source, there are commercial applications of these breeding tools. However, the most important applications will be those that improve the lives of those who depend upon cassava for their daily calorie intake. "With the first cassava genome in hand, we can cheaply and quickly sequence other varieties that will give us thousands of little signposts – mile markers if you like – that will help us identify key genes for increasing the plant's resistance to the virus," Rounsley said. "The information contained in the cassava genome will provide tremendous opportunities to improve this important crop, bringing it into the mainstream of plant research thereby reducing the time and cost of delivering improved cultivars to farmers who need it most." Countries focused on Cassava: In Thailand, cassava production expanded rapidly in the 1970s and 1980s in response to an ever- increasing demand for cassava pellets used as an energy source for animal feed in Western Europe. The country's cassava production area, initially located in southern Thailand, first moved to the eastern seaboard provinces of Chon Buri and Rayong during the late 1970s, and in the 1980s expanded greatly in the Northeast. During the late 1980s, Thailand's cassava-production area covered 10 million rai. Almost all of this was destined for the lucrative export market for cassava pellets in Europe. However, changes in the EU's agricultural policies in 1993 lowered the support price of their own grain crops, and made Thailand's cassava pellets no longer competitive as a cheap source of energy in animal-feed rations. Thus, the amount of cassava pellets Thailand exported to the EU began to drop precipitously year after year and is now less than 400,000 tons. Foreseeing the problem of overproduction, the Thai government tried to decrease the cassava-growing area by encouraging farmers to plant other crops, however, none of these were as well adapted to the climatic conditions in the Northeast as cassava. As a result, farmers continued to grow cassava, albeit
  • 13. in a much reduced area of about 6.2 million rai. But while the area was reduced, cassava yields started to increase substantially from about 2.24 tons per rai in 1995 to 3.55 tons per rai in 2006/2007. The result was that total cassava production decreased only marginally from a peak of 24 million tons in 1989 to about 16 million tons in 1998/1999 and back up to 25 million tons in 2006/2007. So, what does Thailand do with 25 million tons of cassava roots? First, the Thai cassava industry quickly changed from making mainly cassava pellets for export to making more and more cassava starch for both the domestic and export markets. Currently the cassava starch and modified starch industry absorbs over 50 per cent of all cassava roots produced in the country, as compared to 36 per cent in 1991. Secondly, our Chinese neighbours to the north have also built more and more starch factories, to the point that domestic production could not keep up with demand. Thus, in 2001, they started importing dry cassava chips from Thailand, first in very modest amounts, but increasing every year to four million tons in 2006. Finally, in 2000, Thailand was one of the first countries in Asia to initiate a "gasohol" or E10 programme, with the aim of replacing 10 per cent of normal gasoline with fuel-ethanol, which is a renewable energy source made from locally produced sugarcane (or molasses), maize or cassava. Presently 15 factories produce a total of 3.4 million liters of ethanol per day. Thailand currently has the second highest cassava yield after India and nearly double the average yield in the world. The rapid increase in the country's cassava yield was achieved through the hard work and excellent collaboration among the Agriculture Department, the Agriculture Extension Department and Kasetsart University as well as with the private processing and trading sector and the Thai Tapioca Development Institute. So what does the future hold for cassava in Asia? In many countries the increasing demand for cassava roots can only be satisfied through marked increases in yield. This will require renewed efforts in breeding, agronomy, biotechnology and improvements in processing technologies, coupled with a dynamic and effective extension programme using a farmer participatory approach. Even though cassava is the third most important food crop in Southeast Asia after rice and maize, it has always been considered as an "orphan crop", with little funding allocated for research of the crop. While there are thousands of researchers all over the world working on important crops like rice, maize, soybean, oil palm and rubber, there are only a few dozen researchers working on cassava. Unless this situation improves and the crop receives adequate funding and research attention, it will remain an "orphan crop", only grown by the poorest farmers and eaten by the poorest people, except that the increased demand for fuel-ethanol, if not met through rapid increases in production, will push up the price until the poor will no longer be able to afford it. In Venezuela, a team of researchers on the Yaretanol project report that they have developed a means to convert cassava waste to ethanol, butanol, isobutanol and propanol. “Yare” is the local term for the milky byproduct of cassava root food production, a substance high in cyanide content. The team said that 2 cubic centimeters of Yare can kill an animal weighing over 1,000 pounds. The Yare conversion process, according to the research team could produce 1 percent of Venezuela’s ethanol, or 3 Mgy. The group also reported that they can use starch and ethanol as a base for biopolymers, and plastic extract as a base for bio-combustibles. China-based Hainan Yedao Group has reportedly invested $51.5m (£31.8m) in a new biofuel facility that is expected to produce 33 million gallons a year of bio-ethanol from cassava plants. The plans follow recent moves from the Beijing government to ban the use of grain-based energy crops in bio- ethanol, amid concerns demand had led to a decline in food supplies. Cassava avoids the ban as it is a root vegetable that represents China's fifth-largest crop yield after rice, sweet potato, sugar cane and maize. The Guangxi region, next to where the plant is located, accounts for 70 per cent of the country's yield, averaging seven million tons a year.
  • 14. Traditionally ethanol produced from cassava is used for food and pharmaceutical purposes, but it is increasingly being touted as a more sustainable alternative to first-generation biofuels. China's Beihai Gofar Marine Biological Industry has also announced plans for a 100,000 ton-per-year cassava-based ethanol plant in the Guangxi region. The government has also signalled its support for the biofuel sector, with 10 of the country's 22 provinces in China mandating the use of ethanol-blended gasoline in cars. China will build its largest production base of non-grain bio-ethanol fuel in a southern province to mix with or replace the gasoline and diesel oil for vehicle use, reported Shanghai-based Jiefang Daily last week. Global automakers have seen the promise of the new-generation ethanol fuel development in China. In April, China successfully replaced the gasoline and diesel oil with bio-ethanol fuel in Guangxi Zhuang Autonomous Region, and gas stations in 14 cities of Guangxi started to sell bio-ethanol. Some locally produced fuel ethanol is also mixed in a ratio of 10% with the ordinary gasoline to produce ethanol gasoline. The large ethanol refiner of Guangxi's Beihai city now can produce 200,000 tons of ethanol annually out of about 1.5 million tons of cassava. Guangxi is the first Chinese province to use cassava instead of grain to produce ethanol. Guangxi's output of cassava accounted for more than 60% of the country's total, with an output of 7.8 million tons a year. China has forbidden using grain for ethanol production last year to guarantee the supply of food. Now, about ten other Chinese provinces are using ethanol fuel. The country's ethanol fuel sales will reach 30 million tons in 2010 to make up half of the total gasoline supplies. Ethanol fuel will help China, the world's second largest auto market, ease the shortage of energy supply, and cut the carbon monoxide and carbon dioxide emissions by about 30% and 10% respectively. China may have 60,000 new energy vehicles by 2012 and the government will focus on boosting the annual production of clean-energy vehicles. Agriculture remains the main activity in the developing world. The world’s farmers are fully capable of increasing the amount they produce, but, in order for this to happen, demand must increase. Poverty reduces global food demand and acts as a brake on food production. Most of the world’s poorest people live in rural areas and work the land. Increased demand for agricultural products thus would mean more farm income and less poverty, more productive agriculture, more food, more rural economic development, and reduced migration to cities. Conclusion : Many developing countries still have a significant quantity of land available that is well adapted to rain-fed crops – about as much as now is being farmed (over 1.7 billion acres). These lands do not include areas inhabited by human beings, forests, or protected areas. If a country can produce and export biofuels, it will have a stronger economy and more resources to address the needs of the poor. Africa, with its significant sugar cane/Cassava production potential, is often cited as a region that could profit from experience and technology, although obstacles to realizing it (infrastructure, institutional, etc.) should not be underestimated.