1. PRACTICAL
MICROBIOLOGY
Sterilization, Disinfection , and Antisepsis.
The use of Microscope.

2. Destroying
Microorganism
Sterilization Disinfection Antiseptics

3. Sterilization
Is the removal of all microbes, including endosores.
Can be achieved by mechanical means, heat , chemicals
or radiation.

4. Monitoring steam sterilization

5. • Is the process of eliminates most or all microorganisms,
with the exception of Endospores.
Disinfection
Disinfection
High level
disinfectants
Intermediate
level
disinfectants
Low level
disinfectants

6. • Is the process of destroying or inhibiting the growth of
microorganisms in or on living tissue and can also be
referred as biocides.
Antiseptics

7. • Mechanical methods such as scrubbing with a brush are
most commonly, but alternatively alcohol-based rubs are
also used for hand disinfection.
Disinfection of Hands

8. The use of Microscope

9. The oil immersion objective

10. Oil and glass have the same refractive
index