OVium Bio-Information Solutions use forefront algorithms to analyze key data resources such NCBI, EBLM and PDB to develop cell signal pathways. OVium employs cloud and MPP computing solutions with homology and signal network mapping to develop chemical and protein pathways for discovery research.

1. Computational Bio-Discovery Wolfgang Kraske PhD EE A scientific paradigm Bio-Information Networking Community Tool Services Model Characterization

2. Wolfgang F. Kraske, PhD PhD Electrical Engineering University of Southern California, December 1995 Advisor- Irving S. Reed, PhD, Charles E. Powell Professor Emeritus of E.E./C.S. Research- Geometric Algebra for Topological Information Representation MS Electrical Engineering University of Southern California, December 1986 Research- Microwave Imaging and Signal Processing BS Physics/Mathematics-University of Maryland, UMCP, 1982

3. Computational Bio-Discovery An Awkward Situation Cells in Vivo? Bioinformatic Research System

5. Community Enterprise and Tools

6. Model Characterization Vector/ Small Molecule Sequence Structure Pathway

9. Integration of internal with external scientific communities

10. Integration with external patient and lay communities

12. Wet Laboratory Sequencing and Molecular Data Acquisition Pharmaceutical Research & Development Mathematics and Computer Science Research & Development External Application Computing Resources Community Bio-Information Network Sources

13. PCR Mass Spectrometry Magnetic Resonance Spectroscopy Micro-Array Gel Electrophoresis Protein Rapid Translation Southern Blot X-ray crystallography Computational Bio-Discovery Challenge: Tool Integration Centrifugation Titration Electron Microscopy Light Microscopy IVC Emulsion Phage Display

14. Computational Bio-Discovery Service Architecture Solution Legend Campus Laboratories Firewall REST Server Campus Switch Load Balancing Internet Load Balancing Firewall Entity Servers Database Servers Mass Storage Network Switch Web Segment Data Segment Application Segment External Services Web/Session/Tool Servers Mass Storage Database Server Entity Server Tool Server Web Content Server HTTP Server Social Server Ethernet/ 100/1000 Base T Network Switch Load Balance Server Firewall Server Fiber Channel Rest Server/

16. EC2/3

17. Oracle Metro

18. MS Azure

19. Web Services WF Kraske, Voxar-All ATM Distributed Biomedical Visualization:T3D MPP, 8 th IEEE Symposium on Computer Based Med Systems, 249, 1995 Dell Xeon Cluster, Message Passing Innterface

20. MVC Architecture Fi bers REST-Representation State Transfer Zend Framework

21. Ajax – XMLHttpRequest

22. Iterated Software Lifecycle Requirements Design Development Test Implementation Analysis Deployment Upgrade or New Iteration ... Iterated Weekly Functional Roll out Agile and eXtreme programming processes I Jacobson, G Booch, J Rumbaugh, Unified Software Development Process, Addison Wesley 1999

23. Use Case Management Programmer Program Test & Validation Evaluator Project Manager Report Report Planning Stakeholder Control

24. Conceptual Model Sequence: Linear Small Molecule: Node Structure: Spatial Pathway: Time & Space

28. Internet links

29. Bioinformatic Analysis Algorithms Random Network Scale-Free Network

30. Protein Interactome Knockout of Proteins Eliminates Temporal Communication between Processes Knockout of Scaffold Proteins Eliminate Structure NATURE | doi:10.1038/nature02555 |www.nature.com/nature NATURE | doi:10.1038/nature02555 | www.nature.com/nature 2004, pp 1-6

31. Iterative Construction of Deterministic Scale-Free Networks Peripheral Nodes with 4 Links Hub Nodes with 6 Links A. Barbasi, “Linked”, Penquin Books, pp. 232-237, 2003 Level 0 Level 1 Level 2 Hub Nodes with 15 Links

34. Cluster measures to establish hierarchical structure

35. Regular statistical characterization MEJ Neuman, SH Strogatz, DJ Watt, “Random Graphs with arbitrary degree distributions & applications”, Physical Review E, 64, 026118-1, 2003

38. FGFR

40. Inhibited by Gleevac

41. Serine Threonine Kinases

43. Indirubin Chinese Herbal

44. Transcription & Translation Hox Algorithm Varieties Hox Clock

46. Build, expand and analyze pathways

47. Find relationships among genes, proteins,

48. cell processes and diseases

50. Science Signaling

51. Prolexys HyNet protein-protein

52. Interaction database Probe-target hybridization

54. I have programmed a variety of multi-scale and multi-dimensional algorithms on Cloud and MPP architecture with great success A Gelman,J Hill, “Data Analysis Using Regression & Multilevel/Hierarchical Models”, Cambridge University Press, 2008

57. Genetic Algorithms

59. Translation Codon

60. Human Kinome

61. Montage Pictures

62. Montage Pictures