5. Tumor Genome Analysis Pre- and Post Treatment intact tumor microenvironment GeneChip Human Exon 1.0 ST Array DNA Methylation Analysis other genetic and epigenetic assays Genome-Wide Human SNP Array 6.0
6. matched matched 50 mouse models Repeatable Systematic Preclinical Efficacy Evaluation of Drugs (SPEED) CTRL Arm : 50 Pts Tx Arm : 50 Pts Pathak et al., Am J Trans Res, 2009
8. Purification of Transgene fragments for Microinjectin SPC/Δ3B4Del/IGFP (Δ3B4G) SPC/Δ3B4/IGFP (Δ3B4G) Transgene 10 kb 8 6 4 3 Vector
9. Genotyping Strategy of Transgenic Mice Tail DNA + Δ3B4DG Tail DNA + Δ3B4DG Tail DNA + Δ3B4G Tail DNA + Δ3B4G 100 bp Marker 1 kB Marker Tail DNA Tail DNA Geno#1 Geno#2
10. Generation of Conditional Mutant Cre floxed breeding ATG Promoter Cre Exon3 Exon2 Tissue Specific Conditional KO mice
11. Generation of FloxedHuR Mouse Line 5 ARM (5.1KB) 3 ARM (1.7 KB) 5 ARM probe 3 ARM probe E E A A A A A A E E E E2 E1 E3 Neo PGK-TK Targeting Vector LoxP LoxP LoxP WT Mutant 5ARM (Apl I) 10.0kb 11.3kb 3ARM (EcoRI) 5.5kb 7.5kb