1. Antibiotics: Protein Synthesis, Nucleic Acid Synthesis and Metabolism

5. Antibiotic Susceptibility Testing 8 4 0 2 1 Tetracycline (  g/ml) MIC = 2  g/ml Determination of MIC Chl Amp Ery Str Tet Disk Diffusion Test

6. Zone Diameter Standards for Disk Diffusion Tests

8. Antibiotics that Inhibit Protein Synthesis

9. Review of Initiation of Protein Synthesis 30S 1 3 2 GTP 1 2 3 GTP Initiation Factors mRNA 3 1 2 GTP 30S Initiation Complex f-met-tRNA Spectinomycin Aminoglycosides 1 2 GDP + Pi 50S 70S Initiation Complex A P

10. Review of Elongation of Protein Synthesis GTP A P Tu GTP Tu GDP Ts Ts Tu + GDP Ts Pi P A Tetracycline A P Erythromycin Fusidic Acid Chloramphenicol G GTP G GDP + Pi G GDP A P + GTP

11. Protein Synthesis Microbe Library -American Society for Microbiology www.microbelibrary.org

12. Survey of Antibiotics

14. Antimicrobials that Bind to the 30S Ribosomal Subunit

16. Microbe Library American Society for Microbiology www.microbelibrary.org

20. Antimicrobials that Bind to the 50S Ribosomal Subunit

23. Microbe Library American Society for Microbiology www.microbelibrary.org

24. Antimicrobials that Interfere with Elongation Factors Selectivity due to differences in prokaryotic and eukaryotic elongation factors

26. Inhibitors of Nucleic Acid Synthesis

27. Inhibitors of RNA Synthesis Selectivity due to differences between prokaryotic and eukaryotic RNA polymerase

29. Inhibitors of DNA Synthesis Selectivity due to differences between prokaryotic and eukaryotic enzymes

31. Antimetabolite Antimicrobials

35. Anti-Mycobacterial Antibiotics

41. Microbe Library American Society for Microbiology www.microbelibrary.org