Como parte de la investigaci�n de su tesis, genera una biblioteca de.pdf

•

0 recomendaciones•2 vistas

Un estudiante de posgrado y su compañero de laboratorio crearon bibliotecas de transposones mutantes en E. coli y S. cerevisiae respectivamente usando la misma cantidad de transposones y células. Sin embargo, la biblioteca del compañero fue mucho mayor. A pesar de que la absorción del transposón y su actividad son iguales en ambos organismos, la diferencia en el número de cepas mutantes podría deberse a que S. cerevisiae tiene un mayor número de copias cromosómicas, permitiendo que más transposones se inserten.

Educación

Como parte de la investigacin de su tesis, genera una biblioteca de transposones mutantes en la
bacteria E. coli para usarla en una pantalla gentica. Su compaero de laboratorio est realizando el
mismo proceso en la levadura eucariota S. cerevisiae . Ambos agregan el mismo nmero de
transposones al mismo nmero de clulas, pero se desaniman al descubrir que su compaero de
laboratorio termina con una biblioteca mucho ms grande (es decir, crecen ms transposones
mutantes en sus placas para elegir). Suponga que la levadura y E. coli son igualmente capaces de
absorber el transposn y que el transposn es igualmente activo en ambos organismos. Qu podra
explicar esta diferencia en el nmero de cepas mutantes generadas?

Más contenido relacionado

Más de secunderbadtirumalgi

Code using Java Programming. Show your code and output. Create a person class that has the following attributes as well as appropriate set and get methods: String name, int age, String address. Create a separate class that creates an object of type person and set values for the attributes that object. It would then call get method to retrieve and write those attributes onto a File. The name of the file should be .dat. The program should close the file after writing and open the file again and read and display the values..

Code using Java Programming. Show your code and output.Create a pe.pdf

secunderbadtirumalgi

code in html with div styles 9. Town of 0z Info - Microsoft Interne.pdf

secunderbadtirumalgi

CODE FOR echo_client.c: /* A simple echo client using TCP */ #include #include #include #include #include #include #include #include #include #define SERVER_TCP_PORT 3000 /* well-known port */ #define BUFLEN 256 /* buffer length */ int main(int argc, char **argv) { int n, i, bytes_to_read; int sd, port; struct hostent *hp; struct sockaddr_in server; char *host, *bp, rbuf[BUFLEN], sbuf[BUFLEN]; switch(argc){ case 2: host = argv[1]; port = SERVER_TCP_PORT; break; case 3: host = argv[1]; port = atoi(argv[2]); break; default: fprintf(stderr, "Usage: %s host [port]\n", argv[0]); exit(1); } /* Create a stream socket */ if ((sd = socket(AF_INET, SOCK_STREAM, 0)) == -1) { fprintf(stderr, "Can't creat a socket\n"); exit(1); } bzero((char *)&server, sizeof(struct sockaddr_in)); server.sin_family = AF_INET; server.sin_port = htons(port); if (hp = gethostbyname(host)) bcopy(hp->h_addr, (char *)&server.sin_addr, hp->h_length); else if ( inet_aton(host, (struct in_addr *) &server.sin_addr) ){ fprintf(stderr, "Can't get server's address\n"); exit(1); } /* Connecting to the server */ if (connect(sd, (struct sockaddr *)&server, sizeof(server)) == -1){ fprintf(stderr, "Can't connect \n"); exit(1); } printf("Transmit: \n"); while(n=read(0, sbuf, BUFLEN)){ /* get user message */ write(sd, sbuf, n); /* send it out */ printf("Receive: \n"); bp = rbuf; bytes_to_read = n; while ((i = read(sd, bp, bytes_to_read)) > 0){ bp += i; bytes_to_read -=i; } write(1, rbuf, n); printf("Transmit: \n"); } close(sd); return(0); } CODE FOR echo_server.c: /* A simple echo server using TCP */ #include #include #include #include #include #include #include #include #include #define SERVER_TCP_PORT 3000 /* well-known port */ #define BUFLEN 256 /* buffer length */ int echod(int); void reaper(int); int main(int argc, char **argv) { int sd, new_sd, client_len, port; struct sockaddr_in server, client; switch(argc){ case 1: port = SERVER_TCP_PORT; break; case 2: port = atoi(argv[1]); break; default: fprintf(stderr, "Usage: %s [port]\n", argv[0]); exit(1); } /* Create a stream socket */ if ((sd = socket(AF_INET, SOCK_STREAM, 0)) == -1) { fprintf(stderr, "Can't creat a socket\n"); exit(1); } /* Bind an address to the socket */ bzero((char *)&server, sizeof(struct sockaddr_in)); server.sin_family = AF_INET; server.sin_port = htons(port); server.sin_addr.s_addr = htonl(INADDR_ANY); if (bind(sd, (struct sockaddr *)&server, sizeof(server)) == -1){ fprintf(stderr, "Can't bind name to socket\n"); exit(1); } /* queue up to 5 connect requests */ listen(sd, 5); (void) signal(SIGCHLD, reaper); while(1) { client_len = sizeof(client); new_sd = accept(sd, (struct sockaddr *)&client, &client_len); if(new_sd < 0){ fprintf(stderr, "Can't accept client \n"); exit(1); } switch (fork()){ case 0: /* child */ (void) close(sd); exit(echod(new_sd)); default: /* parent */ (void) close(new_sd); break; case -1: fprintf(stderr, "fork: error\n"); } } } /* echod program */ int echod(int sd) { char *bp, buf[BUFLEN]; int n,.

CODE FOR echo_client.c A simple echo client using TCP #inc.pdf

secunderbadtirumalgi

Coastal Louisiana has been experiencing habitat fragmentation and habitat loss for a significant period of time. This is caused by a combination of various human activities and natural processes that have led to coastal erosion and land loss. Animals, like Brown Pelicans, need these coastal areas to raise their offspring, but the loss of habitat has led to significant reductions in their population sizes. Assuming that the Mississippi River Diversion project can restore much of this habitat AND assuming populations of Brown Pelicans can rebound, which phrase that we discussed best describes this scenario? a. Population Bottleneck b. Founder Effect c. Blending Inheritance d. Frequency-dependent Selection.

Coastal Louisiana has been experiencing habitat fragmentation and ha.pdf

secunderbadtirumalgi

Cloud Solution s had the following accounts and balances as of December 31: Account Debit Credit Cash $ 20,000 Accounts Receivable 2,000 Supplies 1,500 Prepaid Insurance 1,400 Accounts Payable $ 4,000 B. Oscar, Capital 14,900 Lodging Revenue 7,000 Salaries Expense 500 Utilities Expense 500 Totals $ 25,900 $ 25,900 Using the information in the table, calculate the total assets reported on the balance sheet for the period..

Cloud Solutions had the following accounts and balances as of Decemb.pdf

secunderbadtirumalgi

Climate scientists claim that CO2 has risen recently to levels that have not been seen in the history of humankind. How do they know this? Group of answer choices 1 They run computer models backwards from the present into the past; no direct measurments are made 2 They are extrapolating backwards from measurements made in the last few years 3 From the dawn of humankind, people have made measurements of atmospheric CO2, and archeologists have recently discovered these records 4 They are basing this on direct measurements of the atmosphere over the last 60 years or so, and overlapping ice-core data that extend back about 800,000 years 5 It comes from tree ring studies, using a theoretical relationship between temperature and CO2 concentration.

Climate scientists claim that CO2 has risen recently to levels that .pdf

secunderbadtirumalgi

Climate change is one of the defining challenges of our time, but today we have an historic opportunity to change course. The global carbon pricing revenue in 2021 increased by almost 60 percent from 2020 levels, to around $84 billion, providing an important source of funds to help support a sustainable economic recovery, finance broader fiscal reforms, or invest in communities as part of the low-carbon transition future. Required Discuss the role of carbon market in promoting climate finance.

Climate change is one of the defining challenges of our time, but to.pdf

secunderbadtirumalgi

Classify each of the following items as excludable, nonexcludable, r.pdf

secunderbadtirumalgi

Chris is a young moonshine producer in the Tennessee region of Appalachia. After several successful distilling seasons, he decides to invest his profits into a legal venture. Given his interest in technology innovations, he creates MoonshineTech, a corporation that finances research and development of breakthrough technologies. However, the Bureau of Alcohol, Tobacco, Firearms and Explosives (ATF) and the Internal Revenue Service (IRS) have launched an investigation into Chris illicit activities and the sources of capital for MoonshineTech. What types of crime(s) can Chris can be charged with? What rights/protections he and MoonshineTech enjoy under the U.S. Constitution? Explain your answer. Please note: For this discussion there is no need to research federal or state statutes. You should be able to respond to this fact patter based on the material covered this week..

Chris is a young moonshine producer in the Tennessee region of Appal.pdf

secunderbadtirumalgi

choose the right answer only Question 9 What are the four factors of production? Question 9 options: a)Factories, machines, workers, money b) Natural resources, human resources, capital, information c) Water, employees, computers, patents Question 6 What is Organizational Culture? Question 6 options: a) The values of individuals and the shared beliefs that distinguish members of a group b) The accumulation of norms produced by the continuous expression of an orientation in the choices of a given grouping c) The patterns of thought, feeling, and potential action that result from continuous learning.

choose the right answer onlyQuestion 9 What are the four facto.pdf

secunderbadtirumalgi

Choose ONE of the scenarios below and write a problem-solving report about it. 4. Everyone has noticed the effects of social media on inter-personal communication. Many people, especially young ones, are not good with face-to-face communication anymore because of social media. In many homes, communication has become digital. What can be done to solve this problem? Note: Your problem-solving report may describe the following sections: - Heading - Introduction (Background of the problem) - Current condition of the problem - Causes/effects of the problem - Propose a solution.

Choose ONE of the scenarios below and write a problem-solving report.pdf

secunderbadtirumalgi

Charlie Raymond, 65-year-old male who was admitted to a negative pressure room on Med-Surg for COVID precautions. He has a history of COPD, hypertension, diabetes type II, and a recent myocardial infarction. He is a retired postal worker who lives at home with his wife. He is on Claforan (cefotaxime) 2 g IV BID hr and sliding scale insulin. Initially this cardiologist was concerned about congestive heart failure and Mr. Raymond is receiving Furosemide (Lasix) 20 mg IV twice a day for pulmonary edema. Vital Signs: BP is 145/78, Pulse 89 Respirations 24 and slightly labored, Temperature 100.2 SaO2 94% on 2L nasal cannula. The patient/family is fearing the worst due to COVID-19 Pandemic. Select correct nursing concepts based on report Addiction Gas exchange Infection Metabolism Perfusion Sensory perception Charlie Raymond, 65-year-old male who was admitted to a negative pressure room on Med-Surg for COVID precautions. He has a history of COPD, hypertension, diabetes type II, and a recent myocardial infarction. He is a retired postal worker who lives at home with his wife. He is on Claforan (cefotaxime) 2 g IV BID hr and sliding scale insulin. Initially this cardiologist was concerned about congestive heart failure and Mr. Raymond is receiving Furosemide (Lasix) 20 mg IV twice a day for pulmonary edema. Vital Signs: BP is 145/78, Pulse 89 Respirations 24 and slightly labored, Temperature 100.2 SaO2 94% on 2L nasal cannula. The patient/family is fearing the worst due to COVID-19 Pandemic. Select correct nursing concepts based on report Addiction Gas exchange Infection Metabolism Perfusion Sensory perception.

Charlie Raymond, 65-year-old male who was admitted to a negative p.pdf

secunderbadtirumalgi

Choose all that is correct about SWIFT. SWIFT is an international messaging systems that facilitate transactions between financial institutions. Only financial institutions that directly hold a shared account within the network can make transactions with each other. It conventionally takes 1 to 3 business days for transactions to settle in SWIFT. Payment tracking is made possible through the SWIFT global payments initiative solution. Blockchain networks like Ripple net provide an alternative way for financial institutions to make transactions in a more transparent and quicker..

Choose all that is correct about SWIFT.SWIFT is an international m.pdf

secunderbadtirumalgi

Choose a minority, marginalized, or vulnerable population. Describe at least two challenges they face with accessing and moving through the healthcare system (uninsured, underinsured, low literacy, language barrier, etc.). Discuss how a systems approach to proper care coordination would positively impact the challenges you identified. Describe how this approach will meet the patients needs, priorities, and expectations. How can raising the standard of care for underserved populations affect the health care system as a whole?.

Choose a minority, marginalized, or vulnerable population. Describe .pdf

secunderbadtirumalgi

Chapter 15 Summary Case: Search for Truth Legal and Ethical Issues for Health Professionals Case: Code of Silence Characters Mr. Daniel Marcus: Administrative reviewer Magistrate: Hearing officer Counselor Hadrian: Counsel representing Mr. Marcus Mr. Damon Antonio: Nurse reviewer Counselor Luke: Counsel representing the defendants Dr. Machiavelli: Physician reviewer Ms. Ophelia Cicero: Athens Health Center guide Ms. Margaret Galeria: Nurse trainee Dr. Caesar: Patients physician Mr. Bruce Verus: Mr. Marcuss manager Ms. Carol Titus: Mr. Veruss manager Using the communications process in Figure 15-2, how would you describe the values of each character in this case?.

Chapter 15 Summary Case Search for TruthLegal and Ethical Issues .pdf

secunderbadtirumalgi

Children have a relatively fast metabolism of drugs in comparison to adults. Why? -Humans are born with more liver enzymes and they gradually lose them as they age. -Their liver is proportionately larger in comparison to the rest of their body. -They acquire extra metabolic enzymes via breast milk which persist for many years. -The statement is false - the immaturity of a child's liver means it will not be able to metabolise drugs as well as adults..

Children have a relatively fast metabolism of drugs in comparison to.pdf

secunderbadtirumalgi

Chandy/Misra solution In 1984, K. Mani Chandy and J. Misra[5] proposed a different solution to the dining philosopher's problem to allow for arbitrary agents (numbered P1, ..., Pn) to contend for an arbitrary number of resources, unlike Dijkstra's solution. It is also completely distributed and requires no central authority after initialization. However, it violates the requirement that "the philosophers do not speak to each other" (due to the request messages). For every pair of philosophers contending f.

ChandyMisra solution In 1984, K. Mani Chandy and J. Misra[5] propos.pdf

secunderbadtirumalgi

Check all that apply. Which of the following conclusions can be made from Bharath et al. (2020)? Chronic nutrient addition reduced drought resistance in the Great Plains and California Drought recovery was strongly tied to growing season precipitation in California Chronic nutrient addition increased drought recovery in the Great Plains and California Communities with higher species richness were more resistant to drought.

Check all that apply. Which of the following conclusions can be made.pdf

secunderbadtirumalgi

Categorize each of the following activities as to which management responsibility it fulfills: planning, directing, or controlling. Some activities may fulfill more than one responsibility. (Select an "X" in the input field if the management responsibility is fulfilled. If the management responsibility is not fulfilled, leave the input field empty.) Question content area bottom Part 1 Activity Management Responsibility Planning Directing Controlling a. Management decides to increase sales growth by 20.

Categorize each of the following activities as to which management r.pdf

secunderbadtirumalgi

Charles Darwin naci el 12 de febrero de 1809, o sea, hace 212 aos este mircoles. A cul de las siguientes reas de estudio contribuy Darwin? Evolucin, describiendo primero la seleccin natural como un mecanismo para el cambio biolgico. Psicologa y desarrollo humano, mediante el estudio de la expresin y la emocin humana. Botnica, a travs de experimentos sobre fototropismo y un extenso estudio de plantas insectvoras. Geologa y oceanografa describiendo primero la formacin de islas conocidas como atolones. Todas las anteriores y ms! Q2 Las plantas crean energa para el resto del ecosistema. Verdadero FALSO Q3 Por qu las hojas cambian de color a tonos de amarillo, naranja o rojo? Los azcares de la savia llenan las hojas antes del invierno. La clorofila degradada cambia a muchos otros colores. En ausencia de fotosntesis, las hojas producen energa exclusivamente por respiracin celular aerbica. Durante los complejos procesos de abscisin, la clorofila se degrada antes que otros pigmentos, como los carotenoides, que todava estn presentes en las hojas. A. Evolucin, describiendo primero la seleccin natural como un mecanismo para el cambio biolgico. B. Psicologa y desarrollo humano, mediante el estudio de la expresin y la emocin humana. C. Botnica, a travs de experimentos sobre fototropismo y un extenso estudio de plantas insectvoras. D. Geologa y oceanografa describiendo primero la formacin de islas conocidas como atolones. MI. Todas las anteriores y ms!.

Charles Darwin naci� el 12 de febrero de 1809, o sea, hace 212 a�os .pdf

secunderbadtirumalgi

Más de secunderbadtirumalgi (20)