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La motivación es esencial para el éxito y la productividad de una empresa. El verdadero líder sabe cómo motivar a su gente, ya que los recursos humanos motivados son fundamentales para la innovación y la estrategia de una empresa. El arte de motivar al personal es una de las claves del éxito empresarial moderno.
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Para visualizar melhor, baixe o arquivo ".pdf" para seu computador.
Close reading magnetism writing assignment
Magnetism makes people's lives easier in many ways. Students are instructed to write a paragraph with a topic sentence stating how magnetism helps people, at least three details from an article to support this, and a concluding sentence summarizing the main points. The document provides a template for students to write a paragraph explaining the various uses of magnetism.
partes de excel 2010
Este documento presenta una unidad sobre la manipulación de celdas en Excel. Explica diferentes métodos para seleccionar, copiar, mover y borrar celdas, así como técnicas para manejar y ordenar datos como eliminar filas duplicadas, validar datos y buscar y reemplazar valores.
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Este documento es una pregunta parlamentaria dirigida a la Comisión Europea sobre una investigación previamente anunciada sobre discriminación lingüística en contratos públicos. La pregunta pregunta si la Comisión inició la investigación anunciada, qué pasos se han dado y si se han propuesto medidas concretas para garantizar la igualdad de oportunidades para los empresarios en esos contratos públicos.
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La motivación es esencial para el éxito y la productividad de una empresa. El verdadero líder sabe cómo motivar a su gente, ya que los recursos humanos motivados son fundamentales para la innovación y la estrategia de una empresa. El arte de motivar al personal es una de las claves del éxito empresarial moderno.
Annotated bibliographies wp
This document summarizes three research articles on the use of cannabis to treat Crohn's disease. The first article finds that 21 out of 30 Crohn's patients saw improved symptoms after treatment with cannabis. It also found reductions in drug usage and surgeries. The second article determines that cannabidiol selectively inhibits inflammatory hypermotility in mice when an inflammatory stimulus is present. The third article surveys Crohn's and ulcerative colitis patients and finds that cannabis helped relieve common symptoms like abdominal pain and diarrhea for most users.
Drug discovery strategy final draft
The document discusses in silico drug discovery methods including identifying potential drug targets, generating pharmacophore models, screening compound databases, and analyzing top hits through molecular docking simulations. A drug discovery strategy is outlined involving primary and secondary screening to identify lead compounds. The work plan describes using AutoDock Vina to dock and rank compounds, selecting the top 14 hits, and analyzing their binding interactions with HIV proteases. Nilotinib, lopinavir, ergoloid, and zafirlukast were evaluated in more detail and found to have high binding affinity to the target proteins.
Em zombage
Bacteriophage Zombage was isolated and characterized using electron microscopy. Pictures were taken of the phage using electron microscopy to view its structure and morphology at high magnification. The electron microscopy images provide detailed views of Zombage's capsid, tail fibers, and other viral structures.
Em shockage
Bacteriophage Shockage was isolated and characterized. Electron microscopy pictures were taken of the phage particles. The pictures show the structure of Shockage, which includes an icosahedral capsid and long, non-contractile tail.
Lab summaries complete
1. This laboratory focused on practicing proper micropipetting techniques. Students mixed solutions of water and dyes to practice using micropipettes accurately and safely. This helped prepare them for upcoming labs requiring precise measurements.
2. Students learned different microscopy techniques, including fluorescence and bright field microscopy. They took photomicrographs using a camera attached to the microscope.
3. Over three days, students extracted their own DNA from mouth cells, ran PCR tests to determine if a patient sample had diabetes, and used gel electrophoresis to analyze protein biomarkers and diagnose a lysosomal storage disorder.
Phages presentation final final final
This document describes the isolation of mycobacteriophages from soil samples in Puerto Rico. Two students isolated three unique phages and characterized them through plaque assays, enrichment techniques, and SDS-PAGE gel electrophoresis. Two distinct phages, named Shockage and Zombage, were identified based on their protein band patterns and plaque morphology. Further sequencing of the phage genomes was proposed to better characterize the newly discovered viruses.
Microscopy. nostoc. brightfield.
This document contains 4 images of the blue-green algae Nostoc viewed under a microscope using Bright Field techniques at magnifications of 200x and 400x. The images show Nostoc in its natural state as well as using a squash technique to better view its structure.
14.anaeli and nicolle. mycobacteriophages paper.
Two mycobacteriophages were isolated from a soil sample collected in Gurabo, Puerto Rico. Following enrichment and plaque purification, two distinct phages (named Shockage and Zombage) were isolated based on differences in morphology and protein band patterns. Shockage had a distinct protein profile while Zombage and another initially isolated phage had nearly identical protein bands, indicating they were the same phage. Further DNA sequencing would help fully characterize these newly isolated phages and determine if they represent unique viruses. Overall, this experiment demonstrated a method for isolating new mycobacteriophages from environmental samples.
Art orieliz
This document summarizes research on a two-component nanoparticle system that uses communication to amplify targeting of nanoparticles to tumors. The system consists of "signalling" nanoparticles that first target tumors and activate the coagulation cascade locally, and "receiving" nanoparticles that target areas of coagulation activation to deliver diagnostic or therapeutic agents. Two types of signalling nanoparticles are investigated - gold nanorods that activate coagulation when heated with near-infrared light, and engineered tissue factor proteins that activate coagulation upon binding to tumor receptors. Both are shown to induce localized fibrin deposition in tumors. Receiving nanoparticles containing imaging or drug payloads are then able to target the coagulated tumor sites for enhanced delivery.
Ss third
This article discusses a study that surveyed patients with inflammatory bowel diseases like Crohn's disease and ulcerative colitis about their use of cannabis. The researchers hypothesized that cannabis works as an anti-inflammatory through receptors called endocannabinoids. The survey found that in most cases, patients reported cannabis helped relieve abdominal pain, diarrhea and reduced appetite. While cannabis has side effects, it improved quality of life for these patients and could be a viable treatment option.
Ss second
This research paper studied whether cannabidiol inhibits inflammatory hypermotility in mice. Researchers induced intestinal inflammation in mice using croton oil, then tested the effects of different doses of cannabidiol. They found that cannabidiol effectively reduced hypermotility when inflammation was present, and higher doses produced greater inhibition. The study concluded that cannabidiol can help normalize intestinal motility during inflammation.
Ss first
This article describes a study of 30 Crohn's disease patients who used cannabis as a treatment. Cannabis is known to have anti-inflammatory properties, and a symptom of Crohn's is bowel inflammation. The study found that 21 of the 30 patients experienced great improvement in their condition after using cannabis. The results show that cannabis treatment can reduce symptoms of Crohn's disease like bowel inflammation.
Yaddilette
Galsulfase is a recombinant form of human N-acetylgalactosamine 4-sulfatase that was approved by the FDA in May 2005 for the treatment of mucopolysaccharidosis type VI (MPS-VI), a rare lysosomal storage disorder. MPS-VI is caused by a deficiency of N–acetylgalactosamine 4-sulfatase, which leads to the accumulation of dermatan sulfate. Clinical trials found that patients treated with galsulfase showed improvements in walking and stair-climbing ability compared to placebo. Galsulfase represents the first approved treatment for MPS-VI and provides an important new therapy for patients suffering from this rare disease.
Seminar yadilette rivera_amherst,mass_oct.18-1 copy
The Biology Honor Society will provide refreshments from 10:30-10:40 in room 106 MMM on Thursday. Yadilette Rivera, an alumna from Univ. of Massachusetts Amherst, will then give a seminar from 10:40-11:20 in room 106 MMM about the challenges of protein crystallography and graduate school.
Reflection on the third seminar
Yadilette's research focuses on treating lysosomal storage disorders, which cause bone abnormalities, through enzyme replacement therapy rather than bone marrow transplantation. Her treatment involves purifying proteins through crystallization and x-ray analysis to determine the replacement enzyme, then injecting patients weekly and monitoring their progress through tests, with some patients showing increased mobility based on walking and climbing tests. This research helps improve the lives of those suffering from these diseases and creates more awareness.
Jessica torres
This document discusses incorporating unnatural amino acids into proteins to expand their functions. It describes how pyrrolysine is used as an orthogonal system to incorporate unnatural amino acids specified by an amber stop codon. Various unnatural amino acids containing alkene and norbornene functional groups were successfully incorporated into GFP and other proteins in E. coli and mammalian cells. These modified proteins could then be site-specifically labeled using bioorthogonal reactions like the thiol-ene and Diels-Alder reactions. This allows proteins to be labeled and studied in live cells with new functions.
Art. jessica
This document describes a new method for site-specifically labeling proteins using genetically encoded norbornene and rapidly reactive tetrazine probes. Key points:
1) A norbornene-containing amino acid is genetically encoded in E. coli and mammalian cells using the pyrrolysyl tRNA synthetase system.
2) A series of tetrazine probes are developed that react rapidly and specifically with norbornene via an inverse electron demand Diels-Alder reaction.
3) The tetrazine-norbornene reaction is shown to be much faster than established bioorthogonal reactions, allowing specific labeling of proteins both in vitro and on mammalian cell surfaces.
Ad jessica torres seminar
Jessica Torres from North Carolina State University will give a seminar on incorporating unnatural amino acids into proteins. Unnatural amino acids allow the expression of proteins with new functions and enable a wide range of studies by using diverse functional groups beyond the 20 common amino acids. Examples include incorporating fluorescent and spectroscopic probes to study biological processes in live cells. The seminar will discuss how introducing unnatural amino acids into proteins, both in vitro and in vivo, is a promising technique that can provide insights into answering biomedical questions relevant to human health. Refreshments will be provided before the talk at 10:30am on Thursday September 20 in room MMM 106.
Second seminar reflection
An improved method of protein labeling uses unnatural amino acids inserted during protein synthesis, which do not interfere with the organism's natural systems. The unnatural amino acids are chosen for their similarity to natural amino acids but are distinct enough to avoid incorporation elsewhere. By adding a stop codon to the unnatural amino acid and inserting it into tRNA, only the desired protein will be labeled without affecting other proteins.
Olga gonzalez1
Rta is a key transcriptional activator that is required for reactivation of Kaposi's sarcoma-associated herpesvirus (KSHV) from latency. Rta stimulates DNA binding of the transcription factor RBP-Jk to KSHV promoter regions containing RBP-Jk binding sites. The interaction between Rta and RBP-Jk leads to recruitment of additional factors and transactivation of immediate early and delayed early genes, establishing the lytic replication cycle. Precise cis-elements including CANT repeats and [A/T]3 motifs that flank the RBP-Jk site contribute to the strength of Rta/RBP-Jk mediated transactivation. Understanding this molecular mechanism of
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Annotated bibliographies wp
This document summarizes three research articles on the use of cannabis to treat Crohn's disease. The first article finds that 21 out of 30 Crohn's patients saw improved symptoms after treatment with cannabis. It also found reductions in drug usage and surgeries. The second article determines that cannabidiol selectively inhibits inflammatory hypermotility in mice when an inflammatory stimulus is present. The third article surveys Crohn's and ulcerative colitis patients and finds that cannabis helped relieve common symptoms like abdominal pain and diarrhea for most users.
Drug discovery strategy final draft
The document discusses in silico drug discovery methods including identifying potential drug targets, generating pharmacophore models, screening compound databases, and analyzing top hits through molecular docking simulations. A drug discovery strategy is outlined involving primary and secondary screening to identify lead compounds. The work plan describes using AutoDock Vina to dock and rank compounds, selecting the top 14 hits, and analyzing their binding interactions with HIV proteases. Nilotinib, lopinavir, ergoloid, and zafirlukast were evaluated in more detail and found to have high binding affinity to the target proteins.
Em zombage
Bacteriophage Zombage was isolated and characterized using electron microscopy. Pictures were taken of the phage using electron microscopy to view its structure and morphology at high magnification. The electron microscopy images provide detailed views of Zombage's capsid, tail fibers, and other viral structures.
Em shockage
Bacteriophage Shockage was isolated and characterized. Electron microscopy pictures were taken of the phage particles. The pictures show the structure of Shockage, which includes an icosahedral capsid and long, non-contractile tail.
Lab summaries complete
1. This laboratory focused on practicing proper micropipetting techniques. Students mixed solutions of water and dyes to practice using micropipettes accurately and safely. This helped prepare them for upcoming labs requiring precise measurements.
2. Students learned different microscopy techniques, including fluorescence and bright field microscopy. They took photomicrographs using a camera attached to the microscope.
3. Over three days, students extracted their own DNA from mouth cells, ran PCR tests to determine if a patient sample had diabetes, and used gel electrophoresis to analyze protein biomarkers and diagnose a lysosomal storage disorder.
Phages presentation final final final
This document describes the isolation of mycobacteriophages from soil samples in Puerto Rico. Two students isolated three unique phages and characterized them through plaque assays, enrichment techniques, and SDS-PAGE gel electrophoresis. Two distinct phages, named Shockage and Zombage, were identified based on their protein band patterns and plaque morphology. Further sequencing of the phage genomes was proposed to better characterize the newly discovered viruses.
Microscopy. nostoc. brightfield.
This document contains 4 images of the blue-green algae Nostoc viewed under a microscope using Bright Field techniques at magnifications of 200x and 400x. The images show Nostoc in its natural state as well as using a squash technique to better view its structure.
14.anaeli and nicolle. mycobacteriophages paper.
Two mycobacteriophages were isolated from a soil sample collected in Gurabo, Puerto Rico. Following enrichment and plaque purification, two distinct phages (named Shockage and Zombage) were isolated based on differences in morphology and protein band patterns. Shockage had a distinct protein profile while Zombage and another initially isolated phage had nearly identical protein bands, indicating they were the same phage. Further DNA sequencing would help fully characterize these newly isolated phages and determine if they represent unique viruses. Overall, this experiment demonstrated a method for isolating new mycobacteriophages from environmental samples.
Art orieliz
This document summarizes research on a two-component nanoparticle system that uses communication to amplify targeting of nanoparticles to tumors. The system consists of "signalling" nanoparticles that first target tumors and activate the coagulation cascade locally, and "receiving" nanoparticles that target areas of coagulation activation to deliver diagnostic or therapeutic agents. Two types of signalling nanoparticles are investigated - gold nanorods that activate coagulation when heated with near-infrared light, and engineered tissue factor proteins that activate coagulation upon binding to tumor receptors. Both are shown to induce localized fibrin deposition in tumors. Receiving nanoparticles containing imaging or drug payloads are then able to target the coagulated tumor sites for enhanced delivery.
Ss third
This article discusses a study that surveyed patients with inflammatory bowel diseases like Crohn's disease and ulcerative colitis about their use of cannabis. The researchers hypothesized that cannabis works as an anti-inflammatory through receptors called endocannabinoids. The survey found that in most cases, patients reported cannabis helped relieve abdominal pain, diarrhea and reduced appetite. While cannabis has side effects, it improved quality of life for these patients and could be a viable treatment option.
Ss second
This research paper studied whether cannabidiol inhibits inflammatory hypermotility in mice. Researchers induced intestinal inflammation in mice using croton oil, then tested the effects of different doses of cannabidiol. They found that cannabidiol effectively reduced hypermotility when inflammation was present, and higher doses produced greater inhibition. The study concluded that cannabidiol can help normalize intestinal motility during inflammation.
Ss first
This article describes a study of 30 Crohn's disease patients who used cannabis as a treatment. Cannabis is known to have anti-inflammatory properties, and a symptom of Crohn's is bowel inflammation. The study found that 21 of the 30 patients experienced great improvement in their condition after using cannabis. The results show that cannabis treatment can reduce symptoms of Crohn's disease like bowel inflammation.
Yaddilette
Galsulfase is a recombinant form of human N-acetylgalactosamine 4-sulfatase that was approved by the FDA in May 2005 for the treatment of mucopolysaccharidosis type VI (MPS-VI), a rare lysosomal storage disorder. MPS-VI is caused by a deficiency of N–acetylgalactosamine 4-sulfatase, which leads to the accumulation of dermatan sulfate. Clinical trials found that patients treated with galsulfase showed improvements in walking and stair-climbing ability compared to placebo. Galsulfase represents the first approved treatment for MPS-VI and provides an important new therapy for patients suffering from this rare disease.
Seminar yadilette rivera_amherst,mass_oct.18-1 copy
The Biology Honor Society will provide refreshments from 10:30-10:40 in room 106 MMM on Thursday. Yadilette Rivera, an alumna from Univ. of Massachusetts Amherst, will then give a seminar from 10:40-11:20 in room 106 MMM about the challenges of protein crystallography and graduate school.
Reflection on the third seminar
Yadilette's research focuses on treating lysosomal storage disorders, which cause bone abnormalities, through enzyme replacement therapy rather than bone marrow transplantation. Her treatment involves purifying proteins through crystallization and x-ray analysis to determine the replacement enzyme, then injecting patients weekly and monitoring their progress through tests, with some patients showing increased mobility based on walking and climbing tests. This research helps improve the lives of those suffering from these diseases and creates more awareness.
Jessica torres
This document discusses incorporating unnatural amino acids into proteins to expand their functions. It describes how pyrrolysine is used as an orthogonal system to incorporate unnatural amino acids specified by an amber stop codon. Various unnatural amino acids containing alkene and norbornene functional groups were successfully incorporated into GFP and other proteins in E. coli and mammalian cells. These modified proteins could then be site-specifically labeled using bioorthogonal reactions like the thiol-ene and Diels-Alder reactions. This allows proteins to be labeled and studied in live cells with new functions.
Art. jessica
This document describes a new method for site-specifically labeling proteins using genetically encoded norbornene and rapidly reactive tetrazine probes. Key points:
1) A norbornene-containing amino acid is genetically encoded in E. coli and mammalian cells using the pyrrolysyl tRNA synthetase system.
2) A series of tetrazine probes are developed that react rapidly and specifically with norbornene via an inverse electron demand Diels-Alder reaction.
3) The tetrazine-norbornene reaction is shown to be much faster than established bioorthogonal reactions, allowing specific labeling of proteins both in vitro and on mammalian cell surfaces.
Ad jessica torres seminar
Jessica Torres from North Carolina State University will give a seminar on incorporating unnatural amino acids into proteins. Unnatural amino acids allow the expression of proteins with new functions and enable a wide range of studies by using diverse functional groups beyond the 20 common amino acids. Examples include incorporating fluorescent and spectroscopic probes to study biological processes in live cells. The seminar will discuss how introducing unnatural amino acids into proteins, both in vitro and in vivo, is a promising technique that can provide insights into answering biomedical questions relevant to human health. Refreshments will be provided before the talk at 10:30am on Thursday September 20 in room MMM 106.
Second seminar reflection
An improved method of protein labeling uses unnatural amino acids inserted during protein synthesis, which do not interfere with the organism's natural systems. The unnatural amino acids are chosen for their similarity to natural amino acids but are distinct enough to avoid incorporation elsewhere. By adding a stop codon to the unnatural amino acid and inserting it into tRNA, only the desired protein will be labeled without affecting other proteins.
Olga gonzalez1
Rta is a key transcriptional activator that is required for reactivation of Kaposi's sarcoma-associated herpesvirus (KSHV) from latency. Rta stimulates DNA binding of the transcription factor RBP-Jk to KSHV promoter regions containing RBP-Jk binding sites. The interaction between Rta and RBP-Jk leads to recruitment of additional factors and transactivation of immediate early and delayed early genes, establishing the lytic replication cycle. Precise cis-elements including CANT repeats and [A/T]3 motifs that flank the RBP-Jk site contribute to the strength of Rta/RBP-Jk mediated transactivation. Understanding this molecular mechanism of
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