1. MOTILIDAD Y VIABILIDAD DE ESPERMATOZOIDES,
COMPARACIÓN DE MODELOS DE MAMÍFEROS Y PECES
(Motility and viability of sperm, models of mammalian and
fish comparison)
Jorge Parodi
jparodi@uct.cl
http://nucleo-nipa.uct.cl/
2. Temas
• Viabilidad
• Motilidad y acrosoma
• Estamos explorando
Corrientes
Xenobioticos
3. Cuevas et al 2013
Ren & Xia 2010
Martínez et al 2014, enviado
4. Semen colection
Refrigeration
.AVI
Video conversion Video Capture
Activation of
sperm
Video Editing Analysis with CASA Results
Guerra et al 2014
5. D E
Control Activated
600
400
200
0
*
VCL
% of control
Control Activated
500
400
300
200
100
0
*
VSL
% of control
Martinez et al 2014, enviado
6. A
B
Control 3 days 20 days
2 μm
C
0 2 4 6 8 10 12
120
80
40
0
* * * * * *
Days
Live cells
% of control
* * * *
0 2 4 6 8 10 12
120
80
40
0
Days
Total Motility
normalized (%)
Martínez et al 2014, enviado
10. Day 1
A B
Day 20
5 μm
C D
Control
4 hrs
120
80
40
* * * * *
150 Calcium
Control
2 hrs
4 hrs
6 hrs
100
50
0
without Calcium
*
*
Viability
% of control
4 hrs/not calcium
Martínez et al 2014, enviado
0 2 4 6 8 10 12
0
Days
Calcium Relative Fluorescence Unit
% of control
11. Control
Post activation
Aging
5
4
3
2
1
1.5
1.0
0.5
0.0
*
*
A B
Calcium Relative Fluorescence Unit
% of control
Activated
II Activated
Zero calcium
Activated
C D
Control
Activated
Activated II
0
*
Calcium Relative Fluorescence Unit
% of control
5 *
Control
zero calcium
Calcium add
4
3
2
1
0
*
Calcium Relative Fluorescence Unit
% of control
Martinez et al 2014, enviado
12. A B
150
100
50
Calcium
Calcium recovery
*
*
*
-2 0 2 4 6 8
Total Motility
normalized (%)
150
100
50
Control Calcium add
100
80
60
40
20
0
Progresive Motility
normalized (%)
Control
4 hrs
Without Calcium
4 hrs calcium add
0
*
*
Total Motility
normalized (%)
C
Martinez et al 2014, enviado
13. 150 Calcium
Control
2 hrs
4 hrs
6 hrs
100
50
0
Without Calcium
* *
Intact Acrosomes
normalized (%)
Control Calcium add
150
100
50
0
VCL
% of control
Control Calcium add
100
80
60
40
20
0
VSL
% of control
Calcium W/Calcium
A
B
C D
Martínez et al 2014, enviado
21. Agradecimientos
Dr. Fernando Romero, UFRO
Dr. Ataulfo Martinez-Torres UNAM
Dr. Alfredo Ramirez, UACH
Fondo MECESUP 0804
Asistencias Técnicas 278-2450 AVIMEX
278-2532 Tonalli Ltda
278-2472 EcoHyd
Laboratorio de Fisiología de la Reproducción
Guillermo Guerra
M.V. Pamela Olivares
Colaboradores
Dr. Alexander Ortloff
Dr. Ivan Valdevenito, BIOACUI
http://www.facebook.com/LaboratorioDeFisiologiaDeLaReproduccionLfr