1. ADN Recombinante e Ingieniería Genética Capítulo 16

9. Preparando ADN Recombinante 5’ 3’ G C T T A A A A T T C G G A A T T C C T T A A G 3’ 5’ one DNA fragment another DNA fragment 3’ 5’ In-text figure Page 254

10. nick 5’ 3’ 3’ 5’ G A A T T C C T T A A G nick G A A T T C C T T A A G DNA ligase action In-text figure Page 254

12. DNA fragments + enzymes recombinant plasmids host cells containing recombinant plasmids Figure 16.4 Page 255

14. Preparando ADNc mRNA transcript mRNA–cDNA hybrid single-stranded cDNA double-stranded cDNA Figure 16.5 Page 255

17. Polymerase Chain Reaction Double-stranded DNA to copy Figure 16.6 Page 256 Stepped Art DNA heated to 90°– 94°C Primers added to base-pair with ends Mixture cooled; base-pairing of primers and ends of DNA strands DNA polymerases assemble new DNA strands

18. Polymerase Chain Reaction Figure 16.6 Page 256 Stepped Art Mixture heated again; makes all DNA fragments unwind Mixture cooled; base-pairing between primers and ends of single DNA strands DNA polymerase action again doubles number of identical DNA fragments

26. Huellas genéticas con sondas multilocus

34. Uso de una sonda Colonies on plate Cells adhere to filter Cells are lysed; DNA sticks to filter Probe is added Location where probe binds forms dark spot on film, indicates colony with gene Figure 16.9 Page 259

43. Anormalidades cromosómicas CLONES Enfermedades ADNc ADNg Familias Ligamiento Híbridos celulares FISH Clones contiguos etc. MAPA DEL GENOMA HUMANO Mapa físico Mapa genético Secuenciación Identificación de genes Mapa RATON P o l i m o r f i s m o s

45. 08_01.jpg Proyecto Genoma Humano