Este documento describe un estudio sobre el aumento de la resistencia a múltiples antibióticos de Pseudomonas aeruginosa (P. aeruginosa MDR) como agente infeccioso. Se analizaron 59 pacientes hospitalizados con P. aeruginosa MDR detectada en cultivos de orina y muestras respiratorias entre 2006 y 2009. Se caracterizaron los aislamientos bacterianos y se evaluaron factores de riesgo asociados mediante pruebas genéticas y análisis estadísticos.

1. Laura María Pineda Jiménez Maria Sarah Restrepo Medicina UPB 2011

6. MATERIALES Y MÉTODOS P. Aeruginosa resistente a: ANTIBIÓTICO CMI ACCIÓN Imipenem 16 mg / L Inhibe síntesis de la pared bacteriana. Estable en presencia de betalactamasas Levofloxacino 8 mg / L Inhibe enzimas de la replicación y transcripción del ADN bacteriano Amikacina 64 mg / L Se une la subunidad 30S del ribosoma bacteriano, impide lectura del ARNm Colistina - Cambia permeabilidad de la membrana citoplasmática barcteriana

11. MATERIALES Y MÉTODOS

19. DISCUSSION AUTHOR RESULTS YES NO Tsuji A, et al MDR P. aeruginosa was mostly isolated from urine samples, ranging from 44% to 73% Hota S, et al The respiratory tract became the main source of MDR P. aeruginosa isolation in the ICU, accounting for around 50% Rossolini GM, et al High mortality ranging from 40% to more than 60% has been reported in bacterial nosocomial pneumonia and ventilator associated pneumonia Nishio H, et al In Japan, the predominant type of MBL is IMP-1, whereas VIM-2 is rarely detected, accounting for less than 10%

20. CONCLUSION 1. The P. Aeruginosa is one of the most serious nosocomial pathogens, which survives in wet environments and causes infections like pneumonia. 2. The most affected patients are the ones that have critically illness and immunocompromised, in those patients the P. Aeruginosa mainly colonises the respiratory tract.

21. CONCLUSION 3. The P. Aeruginosa MDR is associated with an increased in the mortality because it produces metallo-b-lactamases (MBL) that inhibits the effect of the antiobiotics, this is problem that in the future is going to affect us all. 4. The reinforcement in disinfection and hygienic precautions reduced the number of patients with MDR P. Aeruginosa infections. However, it continued to colonise high risk inpatients.

22. MAPA CONCEPTUAL #1 Laura María Pineda Jiménez

23. MAPA CONCEPTUAL #2 Maria Sarah Restrepo

25. GRACIAS