2. • RMS is the most common soft-tissue
sarcoma in children.
• Pathogenesis of RMS is associated
with aggressive growth pattern and
increased risk of morbidity and
mortality.
Subtypes:
★ Embryonal: is characterized by
distinct molecular aberrations,
including alterations in the activity
of certain protein kinases.
★ Alveolar
2
Rhabdomyosarcoma (RMS)
3. • FAK is a non-receptor tyrosine
kinase that plays a vital role in
focal adhesion assembly to
promote cytoskeleton dynamics
and regulation of cell motility.
• Aberrations of FAK
phosphorylation have been
linked to the pathogenesis of
different types of cancers
• The activation of this kinase
requires autophosphorylation of
Tyrosine 397
3
Focal adhesion kinase (FAK)
4. OBJETIVE
4
This study aimed to
investigate
the focal adhesion kinase
phosphorylation as a
regulator of embryonal
rhabdomyosarcoma
5. Se utilizó una línea
celular RD de
rabdomiosarcoma de
músculo embrionario
humano, establecida
como modelo para
examinar los perfiles
de fosforilación de
FAK para caracterizar
su papel en la
patogénesis de RMS.
5
Materiales
y métodos
6. 6
● Observar la
inhibición del
crecimiento celular,
registrar el daño o
muerte celular
● Examinar los
efectos de la
inhibición de FAK
sobre la viabilidad
celular RD.
Citotoxicidad
¿Para qué?
Fundamento
Materiales y métodos
7. 7
Western blot Fundamento
Técnica analítica
usada para detectar
una proteína
específica en función
de su peso molecular
mediante el uso de
anticuerpos
¿Para qué?
Detectar el
estado de
fosforilación de
FAK en células
RD y hTERT
Materiales y métodos
9. 9
¿Para qué?
Fundamento
Citometría de flujo
Permite analizar una
población celular
proporcionando una
amplia variedad de
parámetros como el
tamaño celular y la
densidad de epítopes
Analizar el ciclo
celular y el número
de células en cada
fase
Materiales y métodos
14. Discussion
14
Author Contribution
Cohen and Guan Regulation of FAK phosphorylation and
localization are highly dynamic mechanisms that
promote various numbers of cellular activities
under physiological conditions
Schaller FAK is a cytoplasmic non-receptor protein
tyrosine kinase that transmits upstream
extracellular signals to regulate cellular
activities, such as cell proliferation and migration
Gabarra-Niecko FAK plays an important role during different
developmental stages and is highly linked to the
pathogenesis of various tumors
or
15. Conclusions
15
This study in vitro suggest a
key role for FAK in cell
proliferation signals, cell
cycle regulation, and cell
migration activities of RMS
Data presented herein
demonstrate the efficacy of FAK
inhibition using PF-562271 to
suppress RD cell migration
mechanism by attenuating FAK
phosphorylation at Tyr397 site.
