El sistema inmunitario es un conjunto de elementos celulares y moleculares que actuan de manera coordinada para garantizar la homeostasis del organismo en su interacción con el medio ambiente. En este proceso, es crucial el reconocimiento de patrones moleculares relacionados con inducción de patología, incluyendo (pero no restringido excllusivamente a) agentes infecciosos.
1. Fisiología elemental de la sangre
y del sistema inmunitario
Fredy RS Gutierrez MD, MSc, PhD
Universidad Antonio Nariño
Universidade de Sao Paulo
University of Cincinnati
Universidad Industrial de Santander
2. El sistema inmunitario
Treating Infectious Diseases in a Microbial World: Report of Two Workshops on Novel Antimicrobial Therapeutics. National Research Council (US)
Committee on New Directions in the Study of Antimicrobial Therapeutics: New Classes of Antimicrobials; National Research Council (US) Committee on New
Directions in the Study of Antimicrobial Therapeutics: Immunomodulation. Washington (DC): National Academies Press (US); 2006.
15. Tejido linfoide asociado al intestino
Immunobiology: The Immune System in Health and Disease. 5th edition. Janeway CA Jr, Travers P, Walport M, et al. New York: Garland Science; 2001.
16. Nódulo linfático
Immunobiology: The Immune System in Health and Disease. 5th edition. Janeway CA Jr, Travers P, Walport M, et al. New York: Garland Science; 2001.
17. Fisiología del sistema inmunitario
• Sistema Innato
– Barreras
• Físicas
• Químicas
– Fagocitos
• Explosión oxidativa
• Privación de oligoelementos
– Natural Killer
– Linfocitos Tgd
– Factores solubles
• Complemento
• Sistema de Coagulación
• Interferones
• Defensinas
• Citoquinas
• Quimioquinas
– Respuesta inflamatoria
• Sistema Adaptativo
27. Fisiología del sistema inmunitario
• Sistema Innato
• Sistema Adaptativo (adquirido)
– Linfocitos T
• TCD4
– Citoquinas
• TCD8
– Perforinas, granzimas
– Citoquinas
• T Reguladoras
– Citoquinas
– Privación de Triptófano
– Inducción de apoptosis
– Linfocitos B
– NKT
32. Selección clonal
Immunobiology: The Immune System in Health and Disease. 5th edition. Janeway CA Jr, Travers P, Walport M, et al. New York: Garland Science; 2001.
33. Selección clonal
Immunobiology: The Immune System in Health and Disease. 5th edition. Janeway CA Jr, Travers P, Walport M, et al. New York: Garland Science; 2001.
34. Los cuatro principios básicos de la
selección clonal
Immunobiology: The Immune System in Health and Disease. 5th edition. Janeway CA Jr, Travers P, Walport M, et al. New York: Garland Science; 2001.
35. Desarrollo y activación de linfocitos
Molecular Biology of the Cell. 4th edition. Alberts B, Johnson A, Lewis J, et al. New York: Garland Science; 2002.
37. El receptor de linfocitos
Immunobiology: The Immune System in Health and Disease. 5th edition. Janeway CA Jr, Travers P, Walport M, et al. New York: Garland Science; 2001.
42. Respuesta mediada por anticuerpos
Immunobiology: The Immune System in Health and Disease. 5th edition. Janeway CA Jr, Travers P, Walport M, et al. New York: Garland Science; 2001.
48. Regiones hipervariables se encuentran en los
bucles discretos de la estructura plegada
Immunobiology: The Immune System in Health and Disease. 5th edition. Janeway CA Jr, Travers P, Walport M, et al. New York: Garland Science; 2001.
49. Regiones hipervariables se encuentran en los
bucles discretos de la estructura plegada
Immunobiology: The Immune System in Health and Disease. 5th edition. Janeway CA Jr, Travers P, Walport M, et al. New York: Garland Science; 2001.
50. Regiones discretas de
hipervariabilidad en dominios V
Immunobiology: The Immune System in Health and Disease. 5th edition. Janeway CA Jr, Travers P, Walport M, et al. New York: Garland Science; 2001.
51. Sitio de unión de antígenos en la molécula de
anticuerpo
Immunobiology: The Immune System in Health and Disease. 5th edition. Janeway CA Jr, Travers P, Walport M, et al. New York: Garland Science; 2001.
52. Las fuerzas no covalentes que mantienen
unido el complejo antígeno: anticuerpo
Immunobiology: The Immune System in Health and Disease. 5th edition. Janeway CA Jr, Travers P, Walport M, et al. New York: Garland Science; 2001.
57. Agentes patógenos
Immunobiology: The Immune System in Health and Disease. 5th edition. Janeway CA Jr, Travers P, Walport M, et al. New York: Garland Science; 2001.
58. Tipos de microrganismos
que causan enfermedades
Immunobiology: The Immune System in Health and Disease. 5th edition. Janeway CA Jr, Travers P, Walport M, et al. New York: Garland Science; 2001.
59. Tipos de microrganismos
que causan enfermedades
Immunobiology: The Immune System in Health and Disease. 5th edition. Janeway CA Jr, Travers P, Walport M, et al. New York: Garland Science; 2001.
60. Tipos de microrganismos
que causan enfermedades
Immunobiology: The Immune System in Health and Disease. 5th edition. Janeway CA Jr, Travers P, Walport M, et al. New York: Garland Science; 2001.
61. El camino seguido por los linfocitos
entre la linfa y la sangre
Molecular Biology of the Cell. 4th edition. Alberts B, Johnson A, Lewis J, et al. New York: Garland Science; 2002.
63. Infecciones bacterianas desencadenan
una respuesta inflamatoria
Immunobiology: The Immune System in Health and Disease. 5th edition. Janeway CA Jr, Travers P, Walport M, et al. New York: Garland Science; 2001.
69. Respuesta inmune primaria vs secundaria
Molecular Biology of the Cell. 4th edition. Alberts B, Johnson A, Lewis J, et al. New York: Garland Science; 2002.
71. Para la activación de linfocitos
son necesarias dos señales
Immunobiology: The Immune System in Health and Disease. 5th edition. Janeway CA Jr, Travers P, Walport M, et al. New York: Garland Science; 2001.
72. Tolerancia Inmunológica
central & periférica
Molecular Biology of the Cell. 4th edition. Alberts B, Johnson A, Lewis J, et al. New York: Garland Science; 2002.
73. Algunos sitios del cuerpo son
inmunológicamente privilegiados
Immunobiology: The Immune System in Health and Disease. 5th edition. Janeway CA Jr, Travers P, Walport M, et al. New York: Garland Science; 2001.
74. El sistema
inmunitario en la
enfermedad
Inmunodeficiencias primarias
Autoinmunidad
Alergia e hipersensibilidad
Cáncer
75. El sistema inmunitario en la enfermedad
Treating Infectious Diseases in a Microbial World: Report of Two Workshops on Novel Antimicrobial Therapeutics.
National Research Council (US) Committee on New Directions in the Study of Antimicrobial Therapeutics: New Classes of Antimicrobials; National Research
Council (US) Committee on New Directions in the Study of Antimicrobial Therapeutics: Immunomodulation.
Washington (DC): National Academies Press (US); 2006.
82. Vacunas
Immunobiology: The Immune System in Health and Disease. 5th edition. Janeway CA Jr, Travers P, Walport M, et al. New York: Garland Science; 2001.
87. Inmunología aplicada
a la investigación
Biomédica
Ensayos de inmunogenicidad
Ensayos de citotoxicidad
Ensayos de inmunotoxicidad
Modelos animales
Ratones “Knocout”
Citometría e flujo
ELISA
Inmunoprecipitación
Inmunoblot
Modelos matemáticos
88. Ensayo de proliferación antígeno
específica de células T
Immunobiology: The Immune System in Health and Disease. 5th edition. Janeway CA Jr, Travers P, Walport M, et al. New York: Garland Science; 2001.
89. Ensayo de proliferación antígeno
específica de células T
Immunobiology: The Immune System in Health and Disease. 5th edition. Janeway CA Jr, Travers P, Walport M, et al. New York: Garland Science; 2001.
99. Plataformas de
inmunoinformática
• Herramientas computacionales para el estudio
de la respuesta inmunitaria
– http://cytoscape.org/
– http://tools.immuneepitope.org/main/
– http://www-bimas.cit.nih.gov/molbio/hla_bind/
– http://www.epivax.com/
– http://www.imgt.org/
– http://www.ebi.ac.uk/
– http://www.artificial-immune-systems.org/
– …
100. Plataformas de inmunoinformática
Cytoscape
http://cytoscape.org/
Treating Infectious Diseases in a Microbial World: Report of Two Workshops on Novel Antimicrobial Therapeutics.
National Research Council (US) Committee on New Directions in the Study of Antimicrobial Therapeutics: New Classes of Antimicrobials; National Research
Council (US) Committee on New Directions in the Study of Antimicrobial Therapeutics: Immunomodulation.
Washington (DC): National Academies Press (US); 2006.
102. Inmunoingeniería
J. Timmis, P. Andrews, N. Owens and E. Clark Immune Systems and Computation: An Interdisciplinary Adventure.
http://www-users.cs.york.ac.uk/jtimmis/papers/uc08.pdf
103. Inmunoingeniería
Jon Timmis, Emma Hart , Andy Hone , Mark Neal, Adrian Robins, Susan
Stepney and Andy Tyrrell. Immuno-engineering http://www-users.cs.york.ac.uk/jtimmis/immunoengineering.pdf
104. Lecturas recomendadas
• Crivellato E, Vacca A, Ribatti D. Setting the stage: an anatomist's view of the
immune system. Trends Immunol. 2004 Apr;25(4):210-7.
• Abbas AK, Lichtman AH, Pillai S. Cellular and Molecular Immunology 7th Edition.
Capitulo 1 y 2. Elsevier. 2011.
• Janeway. Immunobiology 8th Ed. 2012.
• Delves PJ, Roitt IM. The immune system. First of two parts. Department of
Immunology, Windeyer Institute of Medical Sciences, University College London. N
Engl J Med. 2000 Jul 6;343(1):37-49.
• Delves PJ, Roitt IM. The immune system. Second of two parts. Department of
Immunology, Windeyer Institute of Medical Sciences, University College London. N
Engl J Med. 2000 Jul 6;343(1):37-49.
• Paul WE Fundamental Immunology 6th ed. Capitulo 1 y 2. Wolter Kluwer Health-
Lippincott Williams & Wilkins.
• Flower Darren R. Davies MN. Ranganathan S Inmunomics reviews 3. Bioinformatics
for inmunomics. Springer 2010.
• Korber B, LaBute M, Yusim K. Immunoinformatics comes of age. PLoS Comput Biol.
2006 Jun 30;2(6):e71.
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